Growth-promoting effect on iron-sulfur proteins on axenic cultures of Entamoeba dispar

S. A M Khalifa, E. Imai, Seiki Kobayashi, A. Haghighi, E. Hayakawa, T. Takeuchi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A growth-promoting factor (GPF) that promotes the growth of Entamoeba dispar under axenic culture conditions was found in fractions of mitochondria (Mt), hydrogenosomes (Hg) and chloroplasts (Cp) obtained from cells of six different protozoan, mammalian and plant species. We were able to extract the GPF from the Cp-rich leaf cells of a plant (spiderwort: Commelina communis L.) in an acetone-soluble fraction as a complex of chlorophyll with low molecular weight proteins (molecular weight [MW] approximately 4,600). We also found that on treatment with 0.6 % complexes of 2-mercapthoethonol (2ME), complexes of chlorophyll-a with iron-sulphur (Fe-S) proteins (e.g., ferredoxins [Fd] from spinach and Clostridium pasteurianum) and noncomplex rubredoxin (Rd) from C. pasteurianum have a growth-promoting effect on E. dispar. These findings suggest that E. dispar may lack a sufficient quantity of some essential components of Fe-S proteins, such as Fe-S center.

Original languageEnglish
Pages (from-to)51-58
Number of pages8
JournalParasite
Volume13
Issue number1
Publication statusPublished - 2006 Mar

Fingerprint

Axenic Culture
sulfur proteins
Iron-Sulfur Proteins
Entamoeba
axenic culture
Protein S
Clostridium pasteurianum
Chloroplasts
Intercellular Signaling Peptides and Proteins
Commelina
Tradescantia
Molecular Weight
iron
Ferredoxins
Spinacia oleracea
Plant Cells
Chlorophyll
chloroplasts
Growth
Acetone

Keywords

  • Chloroplasts
  • Entamoeba dispar
  • Growth
  • Hydrogenosomes
  • Iron-sulphur protein
  • Mitochondria

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology

Cite this

Khalifa, S. A. M., Imai, E., Kobayashi, S., Haghighi, A., Hayakawa, E., & Takeuchi, T. (2006). Growth-promoting effect on iron-sulfur proteins on axenic cultures of Entamoeba dispar. Parasite, 13(1), 51-58.

Growth-promoting effect on iron-sulfur proteins on axenic cultures of Entamoeba dispar. / Khalifa, S. A M; Imai, E.; Kobayashi, Seiki; Haghighi, A.; Hayakawa, E.; Takeuchi, T.

In: Parasite, Vol. 13, No. 1, 03.2006, p. 51-58.

Research output: Contribution to journalArticle

Khalifa, SAM, Imai, E, Kobayashi, S, Haghighi, A, Hayakawa, E & Takeuchi, T 2006, 'Growth-promoting effect on iron-sulfur proteins on axenic cultures of Entamoeba dispar', Parasite, vol. 13, no. 1, pp. 51-58.
Khalifa SAM, Imai E, Kobayashi S, Haghighi A, Hayakawa E, Takeuchi T. Growth-promoting effect on iron-sulfur proteins on axenic cultures of Entamoeba dispar. Parasite. 2006 Mar;13(1):51-58.
Khalifa, S. A M ; Imai, E. ; Kobayashi, Seiki ; Haghighi, A. ; Hayakawa, E. ; Takeuchi, T. / Growth-promoting effect on iron-sulfur proteins on axenic cultures of Entamoeba dispar. In: Parasite. 2006 ; Vol. 13, No. 1. pp. 51-58.
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