Growth-promoting effect on iron-sulfur proteins on axenic cultures of Entamoeba dispar

S. A.M. Khalifa, E. Imai, Seiki Kobayashi, A. Haghighi, E. Hayakawa, T. Takeuchi

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3 Citations (Scopus)

Abstract

A growth-promoting factor (GPF) that promotes the growth of Entamoeba dispar under axenic culture conditions was found in fractions of mitochondria (Mt), hydrogenosomes (Hg) and chloroplasts (Cp) obtained from cells of six different protozoan, mammalian and plant species. We were able to extract the GPF from the Cp-rich leaf cells of a plant (spiderwort: Commelina communis L.) in an acetone-soluble fraction as a complex of chlorophyll with low molecular weight proteins (molecular weight [MW] approximately 4,600). We also found that on treatment with 0.6 % complexes of 2-mercapthoethonol (2ME), complexes of chlorophyll-a with iron-sulphur (Fe-S) proteins (e.g., ferredoxins [Fd] from spinach and Clostridium pasteurianum) and noncomplex rubredoxin (Rd) from C. pasteurianum have a growth-promoting effect on E. dispar. These findings suggest that E. dispar may lack a sufficient quantity of some essential components of Fe-S proteins, such as Fe-S center.

Original languageEnglish
Pages (from-to)51-58
Number of pages8
JournalParasite
Volume13
Issue number1
DOIs
Publication statusPublished - 2006 Mar

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Keywords

  • Chloroplasts
  • Entamoeba dispar
  • Growth
  • Hydrogenosomes
  • Iron-sulphur protein
  • Mitochondria

ASJC Scopus subject areas

  • Parasitology
  • Animal Science and Zoology
  • veterinary (miscalleneous)
  • Insect Science
  • Infectious Diseases

Cite this

Khalifa, S. A. M., Imai, E., Kobayashi, S., Haghighi, A., Hayakawa, E., & Takeuchi, T. (2006). Growth-promoting effect on iron-sulfur proteins on axenic cultures of Entamoeba dispar. Parasite, 13(1), 51-58. https://doi.org/10.1051/parasite/2006131051