Growth-promoting effects of the hydrogen-sulfide compounds produced by Desulfovibrio desulfuricans subsp. desulfuricans cocultured with Escherichia coli (DH5α) on the growth of Entamoeba and Endolimax species isolates from swine

Naoko Yoshida, Seiki Kobayashi, Jun Suzuki, Yoshiyuki Azuma, Namiko Kobayashi-Ogata, Dwi Peni Kartikasari, Yasuaki Yanagawa, Satoshi Iwata

Research output: Contribution to journalArticle

Abstract

Certain Desulfovibrio sp. (anaerobic sulfate-reducing bacteria) are indigenous to swine cecum and colon, which are also common habitats for parasitic amoebae such as Entamoeba polecki and Entamoeba suis. In this study, we evaluated the growth-promoting effects of D. desulfuricans co-cultured with Escherichia coli (DH5α) and its products [e.g., hydrogen sulfide (H2S) and certain iron-sulfide (FeS) compounds] using Robinson's medium, on the 4 amoeba isolates from swine-Entamoeba polecki subtype (ST)-1, E. polecki ST-3, Entamoeba suis, and Endolimax sp., and, consequently, a continuous culture system for these amoebae was established. However, this novel culture system was required to regulate the excess H2S dissolved in the medium by increasing air space as amoeba isolates thrive only in large air spaces (30-40%). The effects of air space and H2S and FeS compounds on the growth of E. polecki ST-1 (TDP-5) were determined. E. polecki ST-1 (TDP-5) thrived well in culture bottles with an air space of 30-40% (aerobic) (H2S: ~250-400 μmoles/L), but did not grow at all in an air space < 5% (microaerobic) (H2S:~800 μmoles/L) and in anaerobic vessels (H2S: 20-30 μmoles/L). In both H2S-depleted and FeS compound-depleted conditions, the amoebae sp. could not thrive either. It was hypothesized that an appropriate concentration of H2S and FeS compounds might function as important physiologically active components of electron carriers such as FeS and ferredoxin.

Original languageEnglish
Pages (from-to)402-410
Number of pages9
JournalBioScience Trends
Volume13
Issue number5
DOIs
Publication statusPublished - 2019 Jan 1

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Endolimax
Desulfovibrio desulfuricans
Entamoeba
Hydrogen Sulfide
Amoeba
Escherichia coli
Swine
Air
air
Growth
Desulfovibrio
Ferredoxins
Cecum
Bottles
Sulfates
habitat
Ecosystem
Bacteria
Colon
Electrons

Keywords

  • Desulfovibrio desulfuricans
  • Hydrogensulfide
  • Iron-sulfide compound
  • Robinson's medium
  • Swine amoeba isolate

ASJC Scopus subject areas

  • Health(social science)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Growth-promoting effects of the hydrogen-sulfide compounds produced by Desulfovibrio desulfuricans subsp. desulfuricans cocultured with Escherichia coli (DH5α) on the growth of Entamoeba and Endolimax species isolates from swine. / Yoshida, Naoko; Kobayashi, Seiki; Suzuki, Jun; Azuma, Yoshiyuki; Kobayashi-Ogata, Namiko; Kartikasari, Dwi Peni; Yanagawa, Yasuaki; Iwata, Satoshi.

In: BioScience Trends, Vol. 13, No. 5, 01.01.2019, p. 402-410.

Research output: Contribution to journalArticle

Yoshida, Naoko ; Kobayashi, Seiki ; Suzuki, Jun ; Azuma, Yoshiyuki ; Kobayashi-Ogata, Namiko ; Kartikasari, Dwi Peni ; Yanagawa, Yasuaki ; Iwata, Satoshi. / Growth-promoting effects of the hydrogen-sulfide compounds produced by Desulfovibrio desulfuricans subsp. desulfuricans cocultured with Escherichia coli (DH5α) on the growth of Entamoeba and Endolimax species isolates from swine. In: BioScience Trends. 2019 ; Vol. 13, No. 5. pp. 402-410.
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abstract = "Certain Desulfovibrio sp. (anaerobic sulfate-reducing bacteria) are indigenous to swine cecum and colon, which are also common habitats for parasitic amoebae such as Entamoeba polecki and Entamoeba suis. In this study, we evaluated the growth-promoting effects of D. desulfuricans co-cultured with Escherichia coli (DH5α) and its products [e.g., hydrogen sulfide (H2S) and certain iron-sulfide (FeS) compounds] using Robinson's medium, on the 4 amoeba isolates from swine-Entamoeba polecki subtype (ST)-1, E. polecki ST-3, Entamoeba suis, and Endolimax sp., and, consequently, a continuous culture system for these amoebae was established. However, this novel culture system was required to regulate the excess H2S dissolved in the medium by increasing air space as amoeba isolates thrive only in large air spaces (30-40{\%}). The effects of air space and H2S and FeS compounds on the growth of E. polecki ST-1 (TDP-5) were determined. E. polecki ST-1 (TDP-5) thrived well in culture bottles with an air space of 30-40{\%} (aerobic) (H2S: ~250-400 μmoles/L), but did not grow at all in an air space < 5{\%} (microaerobic) (H2S:~800 μmoles/L) and in anaerobic vessels (H2S: 20-30 μmoles/L). In both H2S-depleted and FeS compound-depleted conditions, the amoebae sp. could not thrive either. It was hypothesized that an appropriate concentration of H2S and FeS compounds might function as important physiologically active components of electron carriers such as FeS and ferredoxin.",
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