Growth Stimulation of Iodide-Oxidizing α-Proteobacteria in Iodide-Rich Environments

Yumi Arakawa, Yukako Akiyama, Hideharu Furukawa, Wataru Suda, Seigo Amachi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

α-Proteobacteria that can oxidize iodide (I -) to molecular iodine (I 2) have only been isolated from iodide-rich natural and artificial environments, i. e., natural gas brine waters and seawaters supplemented with iodide, respectively. To understand the growth characteristics of such iodide-oxidizing bacteria (IOB) under iodide-rich environments, microcosms comprising natural seawater and 1 mM iodide were prepared, and the succession of microbial communities was monitored by culture-independent techniques. PCR-denaturing gradient gel electrophoresis and 16S rRNA gene sequence analysis showed that bacteria closely related with known IOB were predominant in the microcosms after several weeks of incubation. Quantitative PCR analysis targeting specific 16S rRNA gene regions of IOB showed that the relative abundance of IOB in the microcosms was 6-76% of the total bacterial population, whereas that in natural seawater was less than 1%. When 10 3 cells mL -1 of IOB were inoculated into natural seawater supplemented with 0. 1-1 mM iodide, significant growth (cell densities, 10 5-10 6 cells mL -1) and I 2 production (6-32 μM) were observed. Interestingly, similar growth stimulation occurred when 12-44 μM of I 2 was added to seawater, instead of iodide. IOB were found to be more I 2 tolerant than the other heterotrophic bacteria in seawater. These results suggest that I 2 plays a key role in the growth stimulation of IOB in seawater. IOB could potentially attack other bacteria with I 2 to occupy their ecological niche in iodide-rich environments.

Original languageEnglish
Pages (from-to)522-531
Number of pages10
JournalMicrobial Ecology
Volume63
Issue number3
DOIs
Publication statusPublished - 2012 Apr
Externally publishedYes

Fingerprint

Proteobacteria
iodide
iodides
bacterium
bacteria
seawater
microcosm
ribosomal RNA
natural gas
gene
denaturing gradient gel electrophoresis
iodine
quantitative analysis
targeting
brine

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Soil Science

Cite this

Growth Stimulation of Iodide-Oxidizing α-Proteobacteria in Iodide-Rich Environments. / Arakawa, Yumi; Akiyama, Yukako; Furukawa, Hideharu; Suda, Wataru; Amachi, Seigo.

In: Microbial Ecology, Vol. 63, No. 3, 04.2012, p. 522-531.

Research output: Contribution to journalArticle

Arakawa, Y, Akiyama, Y, Furukawa, H, Suda, W & Amachi, S 2012, 'Growth Stimulation of Iodide-Oxidizing α-Proteobacteria in Iodide-Rich Environments', Microbial Ecology, vol. 63, no. 3, pp. 522-531. https://doi.org/10.1007/s00248-011-9986-5
Arakawa, Yumi ; Akiyama, Yukako ; Furukawa, Hideharu ; Suda, Wataru ; Amachi, Seigo. / Growth Stimulation of Iodide-Oxidizing α-Proteobacteria in Iodide-Rich Environments. In: Microbial Ecology. 2012 ; Vol. 63, No. 3. pp. 522-531.
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