GST-P1 as a histological biomarker of synovial sarcoma revealed by proteomics

Yoshiyuki Suehara, Kazutaka Kikuta, Turrent Robert Nakayama, Naobumi Tochigi, Kunihiko Seki, Hitoshi Ichikawa, Kiyonaga Fujii, Tadashi Hasegawa, Tadakazu Shimoda, Hisashi Kurosawa, Hirokazu Chuman, Yasuo Beppu, Akira Kawai, Setsuo Hirohashi, Tadashi Kondo

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Synovial sarcoma have two histological subtypes, biphasic and monophasic, defined respectively by the presence or absence of glandular epithelial differentiation. To develop histological biomarkers for synovial sarcoma subtypes, we examined the proteomic profile using two-dimensional difference gel electrophoresis. We identified 29 protein spots whose intensity was statistically different between the monophasic (15 cases) and biphasic (9 cases) subtypes (p <0.01). Mass spectrometric protein identification demonstrated that these 29 spots corresponded to 24 distinct gene products involved in cytoskeletal organization, trsnscription/translation, protein/collagen binding, and ion transport, as well as structural constituents of the epidermis. Two of the 29 spots derived from glutathione S-transferase P (GST-P1) had higher intensity in biphasic type. Immunohistochemistry on additional 42 synovial sarcoma cases revealed that positive expression of GST-P1 was observed in 10 of 12 biphasic (83.3%), in 4 of 27 monophasic (14.8%) and in 1 of 3 poorly differentiated synovial sarcomas (p = 0.0002). Among the clinico-pathological parameters examined, GST-P1 expression significantly correlated only with the histological subtype. GST-P1 had more discriminative power than the status of fusion genes SYT-SSX1 and SYT-SSX2, previously reported to be correlated with the histological subtype. These results establish GST-P1 as a histological biomarker candidate for synovial sarcoma differentiation into subtypes.

Original languageEnglish
Pages (from-to)623-634
Number of pages12
JournalProteomics - Clinical Applications
Volume3
Issue number5
DOIs
Publication statusPublished - 2009

Fingerprint

Synovial Sarcoma
Biomarkers
Proteomics
Genes
Proteins
Glutathione Transferase
Electrophoresis
Two-Dimensional Difference Gel Electrophoresis
Collagen
Fusion reactions
Gels
Ions
Gene Fusion
Ion Transport
Protein Binding
Epidermis
Immunohistochemistry
SYT-SSX fusion protein

Keywords

  • 2-D DIGE
  • GST-P1
  • Proteomics
  • Synovial sarcoma

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

GST-P1 as a histological biomarker of synovial sarcoma revealed by proteomics. / Suehara, Yoshiyuki; Kikuta, Kazutaka; Nakayama, Turrent Robert; Tochigi, Naobumi; Seki, Kunihiko; Ichikawa, Hitoshi; Fujii, Kiyonaga; Hasegawa, Tadashi; Shimoda, Tadakazu; Kurosawa, Hisashi; Chuman, Hirokazu; Beppu, Yasuo; Kawai, Akira; Hirohashi, Setsuo; Kondo, Tadashi.

In: Proteomics - Clinical Applications, Vol. 3, No. 5, 2009, p. 623-634.

Research output: Contribution to journalArticle

Suehara, Y, Kikuta, K, Nakayama, TR, Tochigi, N, Seki, K, Ichikawa, H, Fujii, K, Hasegawa, T, Shimoda, T, Kurosawa, H, Chuman, H, Beppu, Y, Kawai, A, Hirohashi, S & Kondo, T 2009, 'GST-P1 as a histological biomarker of synovial sarcoma revealed by proteomics', Proteomics - Clinical Applications, vol. 3, no. 5, pp. 623-634. https://doi.org/10.1002/prca.200800211
Suehara, Yoshiyuki ; Kikuta, Kazutaka ; Nakayama, Turrent Robert ; Tochigi, Naobumi ; Seki, Kunihiko ; Ichikawa, Hitoshi ; Fujii, Kiyonaga ; Hasegawa, Tadashi ; Shimoda, Tadakazu ; Kurosawa, Hisashi ; Chuman, Hirokazu ; Beppu, Yasuo ; Kawai, Akira ; Hirohashi, Setsuo ; Kondo, Tadashi. / GST-P1 as a histological biomarker of synovial sarcoma revealed by proteomics. In: Proteomics - Clinical Applications. 2009 ; Vol. 3, No. 5. pp. 623-634.
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AU - Seki, Kunihiko

AU - Ichikawa, Hitoshi

AU - Fujii, Kiyonaga

AU - Hasegawa, Tadashi

AU - Shimoda, Tadakazu

AU - Kurosawa, Hisashi

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AU - Beppu, Yasuo

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AU - Hirohashi, Setsuo

AU - Kondo, Tadashi

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