Guanylyl cyclase and cGMP-specific phosphodiesterase participate in the acrosome reaction of starfish sperm

Osamu Kawase, Seiichi Ueno, Hiroyuki Minakata, Motonori Hoshi, Midori Matsumoto

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In the starfish, Asterias amurensis, the cooperation of three components of the egg jelly, i.e. ARIS (acrosome reaction-inducing substance), Co-ARIS and asterosap, is responsible for inducing the acrosome reaction. Experimentally, ARIS and asterosap are sufficient for the induction. However, when sperm are treated only with asterosap, they become unresponsive to the egg jelly to undergo the reaction. In this study, we analysed the mechanism of the acrosome reaction, using sperm inactivation by asterosap as a clue. Asterosap causes a rapid and transient increase in intracellular cGMP through the activation of the asterosap receptor, a guanylyl cyclase, and causes an increase in intracellular Ca2+. When sperm were pretreated with asterosap, the guanylyl cyclase seemed to be inactivated irreversibly by dephosphorylation. They were still responsive to ARIS but no longer to asterosap. However, in the presence of IBMX or zaprinast, inhibitors against phosphodiesterases (PDEs), they retained their capacity to undergo the acrosome reaction in response to the egg jelly or ARIS alone. IBMX and zaprinast suppressed the intracellular catabolism of cGMP, but not of cAMP. These results suggest that guanylyl cyclase and cGMP-specific, IBMX- and zaprinast-susceptible PDEs are involved in the regulation of the acrosome reaction.

Original languageEnglish
Pages (from-to)345-355
Number of pages11
JournalZygote
Volume12
Issue number4
DOIs
Publication statusPublished - 2004 Nov

Fingerprint

Starfish
Acrosome Reaction
Guanylate Cyclase
Phosphoric Diester Hydrolases
Spermatozoa
1-Methyl-3-isobutylxanthine
Ovum
Guanylate Cyclase-Coupled Receptors
Asterias
Phosphodiesterase Inhibitors

Keywords

  • Acrosome reaction
  • cGMP
  • Guanylyl cyclase
  • Phosphodiesterase
  • Starfish

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Guanylyl cyclase and cGMP-specific phosphodiesterase participate in the acrosome reaction of starfish sperm. / Kawase, Osamu; Ueno, Seiichi; Minakata, Hiroyuki; Hoshi, Motonori; Matsumoto, Midori.

In: Zygote, Vol. 12, No. 4, 11.2004, p. 345-355.

Research output: Contribution to journalArticle

Kawase, Osamu ; Ueno, Seiichi ; Minakata, Hiroyuki ; Hoshi, Motonori ; Matsumoto, Midori. / Guanylyl cyclase and cGMP-specific phosphodiesterase participate in the acrosome reaction of starfish sperm. In: Zygote. 2004 ; Vol. 12, No. 4. pp. 345-355.
@article{b67f555fc4164315a9ad1e87b2f3f3e6,
title = "Guanylyl cyclase and cGMP-specific phosphodiesterase participate in the acrosome reaction of starfish sperm",
abstract = "In the starfish, Asterias amurensis, the cooperation of three components of the egg jelly, i.e. ARIS (acrosome reaction-inducing substance), Co-ARIS and asterosap, is responsible for inducing the acrosome reaction. Experimentally, ARIS and asterosap are sufficient for the induction. However, when sperm are treated only with asterosap, they become unresponsive to the egg jelly to undergo the reaction. In this study, we analysed the mechanism of the acrosome reaction, using sperm inactivation by asterosap as a clue. Asterosap causes a rapid and transient increase in intracellular cGMP through the activation of the asterosap receptor, a guanylyl cyclase, and causes an increase in intracellular Ca2+. When sperm were pretreated with asterosap, the guanylyl cyclase seemed to be inactivated irreversibly by dephosphorylation. They were still responsive to ARIS but no longer to asterosap. However, in the presence of IBMX or zaprinast, inhibitors against phosphodiesterases (PDEs), they retained their capacity to undergo the acrosome reaction in response to the egg jelly or ARIS alone. IBMX and zaprinast suppressed the intracellular catabolism of cGMP, but not of cAMP. These results suggest that guanylyl cyclase and cGMP-specific, IBMX- and zaprinast-susceptible PDEs are involved in the regulation of the acrosome reaction.",
keywords = "Acrosome reaction, cGMP, Guanylyl cyclase, Phosphodiesterase, Starfish",
author = "Osamu Kawase and Seiichi Ueno and Hiroyuki Minakata and Motonori Hoshi and Midori Matsumoto",
year = "2004",
month = "11",
doi = "10.1017/S0967199404002977",
language = "English",
volume = "12",
pages = "345--355",
journal = "Zygote",
issn = "0967-1994",
publisher = "Cambridge University Press",
number = "4",

}

TY - JOUR

T1 - Guanylyl cyclase and cGMP-specific phosphodiesterase participate in the acrosome reaction of starfish sperm

AU - Kawase, Osamu

AU - Ueno, Seiichi

AU - Minakata, Hiroyuki

AU - Hoshi, Motonori

AU - Matsumoto, Midori

PY - 2004/11

Y1 - 2004/11

N2 - In the starfish, Asterias amurensis, the cooperation of three components of the egg jelly, i.e. ARIS (acrosome reaction-inducing substance), Co-ARIS and asterosap, is responsible for inducing the acrosome reaction. Experimentally, ARIS and asterosap are sufficient for the induction. However, when sperm are treated only with asterosap, they become unresponsive to the egg jelly to undergo the reaction. In this study, we analysed the mechanism of the acrosome reaction, using sperm inactivation by asterosap as a clue. Asterosap causes a rapid and transient increase in intracellular cGMP through the activation of the asterosap receptor, a guanylyl cyclase, and causes an increase in intracellular Ca2+. When sperm were pretreated with asterosap, the guanylyl cyclase seemed to be inactivated irreversibly by dephosphorylation. They were still responsive to ARIS but no longer to asterosap. However, in the presence of IBMX or zaprinast, inhibitors against phosphodiesterases (PDEs), they retained their capacity to undergo the acrosome reaction in response to the egg jelly or ARIS alone. IBMX and zaprinast suppressed the intracellular catabolism of cGMP, but not of cAMP. These results suggest that guanylyl cyclase and cGMP-specific, IBMX- and zaprinast-susceptible PDEs are involved in the regulation of the acrosome reaction.

AB - In the starfish, Asterias amurensis, the cooperation of three components of the egg jelly, i.e. ARIS (acrosome reaction-inducing substance), Co-ARIS and asterosap, is responsible for inducing the acrosome reaction. Experimentally, ARIS and asterosap are sufficient for the induction. However, when sperm are treated only with asterosap, they become unresponsive to the egg jelly to undergo the reaction. In this study, we analysed the mechanism of the acrosome reaction, using sperm inactivation by asterosap as a clue. Asterosap causes a rapid and transient increase in intracellular cGMP through the activation of the asterosap receptor, a guanylyl cyclase, and causes an increase in intracellular Ca2+. When sperm were pretreated with asterosap, the guanylyl cyclase seemed to be inactivated irreversibly by dephosphorylation. They were still responsive to ARIS but no longer to asterosap. However, in the presence of IBMX or zaprinast, inhibitors against phosphodiesterases (PDEs), they retained their capacity to undergo the acrosome reaction in response to the egg jelly or ARIS alone. IBMX and zaprinast suppressed the intracellular catabolism of cGMP, but not of cAMP. These results suggest that guanylyl cyclase and cGMP-specific, IBMX- and zaprinast-susceptible PDEs are involved in the regulation of the acrosome reaction.

KW - Acrosome reaction

KW - cGMP

KW - Guanylyl cyclase

KW - Phosphodiesterase

KW - Starfish

UR - http://www.scopus.com/inward/record.url?scp=14844310273&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=14844310273&partnerID=8YFLogxK

U2 - 10.1017/S0967199404002977

DO - 10.1017/S0967199404002977

M3 - Article

C2 - 15751545

AN - SCOPUS:14844310273

VL - 12

SP - 345

EP - 355

JO - Zygote

JF - Zygote

SN - 0967-1994

IS - 4

ER -