GUILLOTINE RAPID FREEZE-CLAMPING OF EXPERIMENTAL ANIMALS.

Yozo Ohnishi; Shingo Hori; Hiroe Nakazawa; Hideaki Yoshino; Shoji Kojima; Ryoko Miyamori; Yoshiro Nakamura; Tadao Hoshino; Muneyuki Horikawa; Kazuo Yamaguchi

GUILLOTINE RAPID FREEZE-CLAMPING OF EXPERIMENTAL ANIMALS.

Yozo Ohnishi, Shingo Hori, Hiroe Nakazawa, Hideaki Yoshino, Shoji Kojima, Ryoko Miyamori, Yoshiro Nakamura, Tadao Hoshino, Muneyuki Horikawa, Kazuo Yamaguchi

Research output: Contribution to journal › Conference article › peer-review

Abstract

A mechanical device is developed which enables the cross-sectioning and freeze-clamping of a target organ in 120 msec in an experimental animal. Because metabolism and flow are rapidly fixed in situ, two dimensional display of metabolism will be possible with biochemical and histochemical techniques. The device consists of: 1) cross-sectioning 2) transfering the sample to the freezing system 3) freezing. In order to prove the usefulness of the device, beating hearts of 7 open chest dogs were cut along the short-axis plane in 4mm width and freeze-clamped to 2. 5 mm width. Anatomical structure and metabolic state (energy charge and NADH fluorescence) of the samples were well preserved. In conclusion, this method gives an adequate simulation model in analyzing the results of three dimensional display of metabolism by PET or NMR methods.

Original language	English
Pages (from-to)	804-805
Number of pages	2
Journal	japanese journal of medical electronics and biological engineering
Volume	22
Publication status	Published - 1984 Apr
Externally published	Yes

ASJC Scopus subject areas

Biomedical Engineering

Cite this

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title = "GUILLOTINE RAPID FREEZE-CLAMPING OF EXPERIMENTAL ANIMALS.",

abstract = "A mechanical device is developed which enables the cross-sectioning and freeze-clamping of a target organ in 120 msec in an experimental animal. Because metabolism and flow are rapidly fixed in situ, two dimensional display of metabolism will be possible with biochemical and histochemical techniques. The device consists of: 1) cross-sectioning 2) transfering the sample to the freezing system 3) freezing. In order to prove the usefulness of the device, beating hearts of 7 open chest dogs were cut along the short-axis plane in 4mm width and freeze-clamped to 2. 5 mm width. Anatomical structure and metabolic state (energy charge and NADH fluorescence) of the samples were well preserved. In conclusion, this method gives an adequate simulation model in analyzing the results of three dimensional display of metabolism by PET or NMR methods.",

author = "Yozo Ohnishi and Shingo Hori and Hiroe Nakazawa and Hideaki Yoshino and Shoji Kojima and Ryoko Miyamori and Yoshiro Nakamura and Tadao Hoshino and Muneyuki Horikawa and Kazuo Yamaguchi",

year = "1984",

month = apr,

language = "English",

volume = "22",

pages = "804--805",

journal = "Japanese Journal of Medical Electronics and Biological Engineering",

issn = "0021-3292",

publisher = "Nihon M E Gakkai",

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TY - JOUR

T1 - GUILLOTINE RAPID FREEZE-CLAMPING OF EXPERIMENTAL ANIMALS.

AU - Ohnishi, Yozo

AU - Hori, Shingo

AU - Nakazawa, Hiroe

AU - Yoshino, Hideaki

AU - Kojima, Shoji

AU - Miyamori, Ryoko

AU - Nakamura, Yoshiro

AU - Hoshino, Tadao

AU - Horikawa, Muneyuki

AU - Yamaguchi, Kazuo

PY - 1984/4

Y1 - 1984/4

N2 - A mechanical device is developed which enables the cross-sectioning and freeze-clamping of a target organ in 120 msec in an experimental animal. Because metabolism and flow are rapidly fixed in situ, two dimensional display of metabolism will be possible with biochemical and histochemical techniques. The device consists of: 1) cross-sectioning 2) transfering the sample to the freezing system 3) freezing. In order to prove the usefulness of the device, beating hearts of 7 open chest dogs were cut along the short-axis plane in 4mm width and freeze-clamped to 2. 5 mm width. Anatomical structure and metabolic state (energy charge and NADH fluorescence) of the samples were well preserved. In conclusion, this method gives an adequate simulation model in analyzing the results of three dimensional display of metabolism by PET or NMR methods.

AB - A mechanical device is developed which enables the cross-sectioning and freeze-clamping of a target organ in 120 msec in an experimental animal. Because metabolism and flow are rapidly fixed in situ, two dimensional display of metabolism will be possible with biochemical and histochemical techniques. The device consists of: 1) cross-sectioning 2) transfering the sample to the freezing system 3) freezing. In order to prove the usefulness of the device, beating hearts of 7 open chest dogs were cut along the short-axis plane in 4mm width and freeze-clamped to 2. 5 mm width. Anatomical structure and metabolic state (energy charge and NADH fluorescence) of the samples were well preserved. In conclusion, this method gives an adequate simulation model in analyzing the results of three dimensional display of metabolism by PET or NMR methods.

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JF - Japanese Journal of Medical Electronics and Biological Engineering

ER -

GUILLOTINE RAPID FREEZE-CLAMPING OF EXPERIMENTAL ANIMALS.

Abstract

ASJC Scopus subject areas

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