TY - JOUR
T1 - H-1. Mg, K-containing microparticle
T2 - A possible active principle of EM fermentation product
AU - Higashinakagawa, Toru
AU - Kiku-Chi, Haruhisa
AU - Kuwayama, Hidekazu
N1 - Funding Information:
This work was supported by the Russian Foundation for Basic Research (# 18-34-00359\18)� References 1� Evdokimova V� et al� The EMBO journal� 2001; 20; 5491-5502� 2� Mordovkina D� A� et al� BBRC� 2016; 480; 629-34� 3� Sutherland B� W� et al� Oncogene� 2005; 24; 4281� 4� Basaki Y� et al� Oncogene� 2007; 26; 2736� 5� Kosnopfel C� et al� Molecular Cancer Research� 2018; 16; 1149-60�
Publisher Copyright:
© 2019, National Academy of Sciences of Ukraine. All rights reserved.
PY - 2019
Y1 - 2019
N2 - EM represents Effective Microorganisms, a microbial consortium consisting mainly of photosynthetic bacteria, lactic acid bacteria and yeast. Various effects on cells of EM fermentation product were reported in the previous WBW25 Meeting in 2017. Here, we report our attempt to identify its active principle and the ongoing preliminary results. The activity was assayed by promoted formation of fruiting body of Dictyostelium discoideum. The EM fermentation product was first subjected to liquid-liquid separation, with the activity being recovered in aqueous phase. Concentrated aqueous fraction was further subjected to column chromatography. No activity was detected in any eluant, while almost all activity was recovered in residual insoluble material. The application of conventional organic chemistry procedures did not lead to any informative results. Acid treatment of the insoluble material produced air bubbles, suggesting it to be composed of some inorganic carbonate. Viewed under scanning electronmicroscope, these residues revealed spherical particles of μm range. Energy Dispersive X-ray (EDX) Spectroscopy pointed to the existence of magnesium and, to a certain extent, potassium. In a separate experiment, acid treatment and alkali neutralization of EM fermentation product completely wiped out the stimulatory activity of fruiting body formation. These lines of evidence indicate these Mg, K-containing microparticle to be active principle of EM fermentation product, at least, in regard to fruiting body formation in Dictyostelium discoideum. How these particles exert their effect is currently under extensive investigation.
AB - EM represents Effective Microorganisms, a microbial consortium consisting mainly of photosynthetic bacteria, lactic acid bacteria and yeast. Various effects on cells of EM fermentation product were reported in the previous WBW25 Meeting in 2017. Here, we report our attempt to identify its active principle and the ongoing preliminary results. The activity was assayed by promoted formation of fruiting body of Dictyostelium discoideum. The EM fermentation product was first subjected to liquid-liquid separation, with the activity being recovered in aqueous phase. Concentrated aqueous fraction was further subjected to column chromatography. No activity was detected in any eluant, while almost all activity was recovered in residual insoluble material. The application of conventional organic chemistry procedures did not lead to any informative results. Acid treatment of the insoluble material produced air bubbles, suggesting it to be composed of some inorganic carbonate. Viewed under scanning electronmicroscope, these residues revealed spherical particles of μm range. Energy Dispersive X-ray (EDX) Spectroscopy pointed to the existence of magnesium and, to a certain extent, potassium. In a separate experiment, acid treatment and alkali neutralization of EM fermentation product completely wiped out the stimulatory activity of fruiting body formation. These lines of evidence indicate these Mg, K-containing microparticle to be active principle of EM fermentation product, at least, in regard to fruiting body formation in Dictyostelium discoideum. How these particles exert their effect is currently under extensive investigation.
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U2 - 10.7124/bc.0009E3
DO - 10.7124/bc.0009E3
M3 - Article
AN - SCOPUS:85073346041
VL - 35
SP - 214
JO - Biopolymers and Cell
JF - Biopolymers and Cell
SN - 0233-7657
IS - 3
ER -