H 2 mediates cardioprotection via involvements of K ATP channels and permeability transition pores of mitochondria in dogs

Akemi Yoshida, Hiroshi Asanuma, Hideyuki Sasaki, Shoji Sanada, Satoru Yamazaki, Yoshihiro Asano, Yoshiro Shinozaki, Hidezo Mori, Akito Shimouchi, Motoaki Sano, Masanori Asakura, Tetsuo Minamino, Seiji Takashima, Masaru Sugimachi, Naoki Mochizuki, Masafumi Kitakaze

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Purpose Inhalation of hydrogen (H 2) gas has been shown to limit infarct size following ischemia-reperfusion injury in rat hearts. However, H 2 gas-induced cardioprotection has not been tested in large animals and the precise cellular mechanism of protection has not been elucidated.We investigated whether opening of mitochondrial ATP-sensitive K+ channels (mKATP) and subsequent inhibition of mitochondrial permeability transition pores (mPTP) mediates the infarct size-limiting effect of H 2 gas in canine hearts. Methods The left anterior descending coronary artery of beagle dogs was occluded for 90 min followed by reperfusion for 6 h. Either 1.3% H 2 or control gas was inhaled from 10 min prior to start of reperfusion until 1 h of reperfusion, in the presence or absence of either 5-hydroxydecanoate (5-HD; a selective mKATP blocker), or atractyloside (Atr; a mPTP opener). Results Systemic hemodynamic parameters did not differ among the groups. Nevertheless, H 2 gas inhalation reduced infarct size normalized by risk area (20.6±2.8% vs. control gas 44.0±2.0%; p>0.001), and administration of either 5- HD or Atr abolished the infarct size-limiting effect of H 2 gas (42.0±2.2% with 5-HD and 45.1±2.7% with Atr; both p>0.001 vs. H 2 group). Neither Atr nor 5-HD affected infarct size per se. Among all groups, NAD content and the number of apoptotic and 8-OHdG positive cells was not significantly different, indicating that the cardioprotection afforded by H 2 was not due to anti-oxidative actions or effects on the NADH dehydrogenase pathway. Conclusions Inhalation of H 2 gas reduces infarct size in canine hearts via opening of mitochondrial KATP channels followed by inhibition of mPTP. H 2 gas may provide an effective adjunct strategy in patients with acute myocardial infarction receiving reperfusion therapy.

Original languageEnglish
Pages (from-to)217-226
Number of pages10
JournalCardiovascular Drugs and Therapy
Volume26
Issue number3
DOIs
Publication statusPublished - 2012 Jun

Fingerprint

Permeability
Mitochondria
Adenosine Triphosphate
Gases
Dogs
Reperfusion
Inhalation
Canidae
Atractyloside
NADH Dehydrogenase
Reperfusion Injury
NAD
Hydrogen
Coronary Vessels
Hemodynamics
Myocardial Infarction
mitochondrial permeability transition pore

Keywords

  • Hydrogen gas
  • Mitochondrial KATP channel
  • Mitochondrial permeability transition pore
  • Myocardial infarction
  • Reperfusion injury

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Cardiology and Cardiovascular Medicine
  • Pharmacology

Cite this

H 2 mediates cardioprotection via involvements of K ATP channels and permeability transition pores of mitochondria in dogs. / Yoshida, Akemi; Asanuma, Hiroshi; Sasaki, Hideyuki; Sanada, Shoji; Yamazaki, Satoru; Asano, Yoshihiro; Shinozaki, Yoshiro; Mori, Hidezo; Shimouchi, Akito; Sano, Motoaki; Asakura, Masanori; Minamino, Tetsuo; Takashima, Seiji; Sugimachi, Masaru; Mochizuki, Naoki; Kitakaze, Masafumi.

In: Cardiovascular Drugs and Therapy, Vol. 26, No. 3, 06.2012, p. 217-226.

Research output: Contribution to journalArticle

Yoshida, A, Asanuma, H, Sasaki, H, Sanada, S, Yamazaki, S, Asano, Y, Shinozaki, Y, Mori, H, Shimouchi, A, Sano, M, Asakura, M, Minamino, T, Takashima, S, Sugimachi, M, Mochizuki, N & Kitakaze, M 2012, 'H 2 mediates cardioprotection via involvements of K ATP channels and permeability transition pores of mitochondria in dogs', Cardiovascular Drugs and Therapy, vol. 26, no. 3, pp. 217-226. https://doi.org/10.1007/s10557-012-6381-5
Yoshida, Akemi ; Asanuma, Hiroshi ; Sasaki, Hideyuki ; Sanada, Shoji ; Yamazaki, Satoru ; Asano, Yoshihiro ; Shinozaki, Yoshiro ; Mori, Hidezo ; Shimouchi, Akito ; Sano, Motoaki ; Asakura, Masanori ; Minamino, Tetsuo ; Takashima, Seiji ; Sugimachi, Masaru ; Mochizuki, Naoki ; Kitakaze, Masafumi. / H 2 mediates cardioprotection via involvements of K ATP channels and permeability transition pores of mitochondria in dogs. In: Cardiovascular Drugs and Therapy. 2012 ; Vol. 26, No. 3. pp. 217-226.
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abstract = "Purpose Inhalation of hydrogen (H 2) gas has been shown to limit infarct size following ischemia-reperfusion injury in rat hearts. However, H 2 gas-induced cardioprotection has not been tested in large animals and the precise cellular mechanism of protection has not been elucidated.We investigated whether opening of mitochondrial ATP-sensitive K+ channels (mKATP) and subsequent inhibition of mitochondrial permeability transition pores (mPTP) mediates the infarct size-limiting effect of H 2 gas in canine hearts. Methods The left anterior descending coronary artery of beagle dogs was occluded for 90 min followed by reperfusion for 6 h. Either 1.3{\%} H 2 or control gas was inhaled from 10 min prior to start of reperfusion until 1 h of reperfusion, in the presence or absence of either 5-hydroxydecanoate (5-HD; a selective mKATP blocker), or atractyloside (Atr; a mPTP opener). Results Systemic hemodynamic parameters did not differ among the groups. Nevertheless, H 2 gas inhalation reduced infarct size normalized by risk area (20.6±2.8{\%} vs. control gas 44.0±2.0{\%}; p>0.001), and administration of either 5- HD or Atr abolished the infarct size-limiting effect of H 2 gas (42.0±2.2{\%} with 5-HD and 45.1±2.7{\%} with Atr; both p>0.001 vs. H 2 group). Neither Atr nor 5-HD affected infarct size per se. Among all groups, NAD content and the number of apoptotic and 8-OHdG positive cells was not significantly different, indicating that the cardioprotection afforded by H 2 was not due to anti-oxidative actions or effects on the NADH dehydrogenase pathway. Conclusions Inhalation of H 2 gas reduces infarct size in canine hearts via opening of mitochondrial KATP channels followed by inhibition of mPTP. H 2 gas may provide an effective adjunct strategy in patients with acute myocardial infarction receiving reperfusion therapy.",
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T1 - H 2 mediates cardioprotection via involvements of K ATP channels and permeability transition pores of mitochondria in dogs

AU - Yoshida, Akemi

AU - Asanuma, Hiroshi

AU - Sasaki, Hideyuki

AU - Sanada, Shoji

AU - Yamazaki, Satoru

AU - Asano, Yoshihiro

AU - Shinozaki, Yoshiro

AU - Mori, Hidezo

AU - Shimouchi, Akito

AU - Sano, Motoaki

AU - Asakura, Masanori

AU - Minamino, Tetsuo

AU - Takashima, Seiji

AU - Sugimachi, Masaru

AU - Mochizuki, Naoki

AU - Kitakaze, Masafumi

PY - 2012/6

Y1 - 2012/6

N2 - Purpose Inhalation of hydrogen (H 2) gas has been shown to limit infarct size following ischemia-reperfusion injury in rat hearts. However, H 2 gas-induced cardioprotection has not been tested in large animals and the precise cellular mechanism of protection has not been elucidated.We investigated whether opening of mitochondrial ATP-sensitive K+ channels (mKATP) and subsequent inhibition of mitochondrial permeability transition pores (mPTP) mediates the infarct size-limiting effect of H 2 gas in canine hearts. Methods The left anterior descending coronary artery of beagle dogs was occluded for 90 min followed by reperfusion for 6 h. Either 1.3% H 2 or control gas was inhaled from 10 min prior to start of reperfusion until 1 h of reperfusion, in the presence or absence of either 5-hydroxydecanoate (5-HD; a selective mKATP blocker), or atractyloside (Atr; a mPTP opener). Results Systemic hemodynamic parameters did not differ among the groups. Nevertheless, H 2 gas inhalation reduced infarct size normalized by risk area (20.6±2.8% vs. control gas 44.0±2.0%; p>0.001), and administration of either 5- HD or Atr abolished the infarct size-limiting effect of H 2 gas (42.0±2.2% with 5-HD and 45.1±2.7% with Atr; both p>0.001 vs. H 2 group). Neither Atr nor 5-HD affected infarct size per se. Among all groups, NAD content and the number of apoptotic and 8-OHdG positive cells was not significantly different, indicating that the cardioprotection afforded by H 2 was not due to anti-oxidative actions or effects on the NADH dehydrogenase pathway. Conclusions Inhalation of H 2 gas reduces infarct size in canine hearts via opening of mitochondrial KATP channels followed by inhibition of mPTP. H 2 gas may provide an effective adjunct strategy in patients with acute myocardial infarction receiving reperfusion therapy.

AB - Purpose Inhalation of hydrogen (H 2) gas has been shown to limit infarct size following ischemia-reperfusion injury in rat hearts. However, H 2 gas-induced cardioprotection has not been tested in large animals and the precise cellular mechanism of protection has not been elucidated.We investigated whether opening of mitochondrial ATP-sensitive K+ channels (mKATP) and subsequent inhibition of mitochondrial permeability transition pores (mPTP) mediates the infarct size-limiting effect of H 2 gas in canine hearts. Methods The left anterior descending coronary artery of beagle dogs was occluded for 90 min followed by reperfusion for 6 h. Either 1.3% H 2 or control gas was inhaled from 10 min prior to start of reperfusion until 1 h of reperfusion, in the presence or absence of either 5-hydroxydecanoate (5-HD; a selective mKATP blocker), or atractyloside (Atr; a mPTP opener). Results Systemic hemodynamic parameters did not differ among the groups. Nevertheless, H 2 gas inhalation reduced infarct size normalized by risk area (20.6±2.8% vs. control gas 44.0±2.0%; p>0.001), and administration of either 5- HD or Atr abolished the infarct size-limiting effect of H 2 gas (42.0±2.2% with 5-HD and 45.1±2.7% with Atr; both p>0.001 vs. H 2 group). Neither Atr nor 5-HD affected infarct size per se. Among all groups, NAD content and the number of apoptotic and 8-OHdG positive cells was not significantly different, indicating that the cardioprotection afforded by H 2 was not due to anti-oxidative actions or effects on the NADH dehydrogenase pathway. Conclusions Inhalation of H 2 gas reduces infarct size in canine hearts via opening of mitochondrial KATP channels followed by inhibition of mPTP. H 2 gas may provide an effective adjunct strategy in patients with acute myocardial infarction receiving reperfusion therapy.

KW - Hydrogen gas

KW - Mitochondrial KATP channel

KW - Mitochondrial permeability transition pore

KW - Myocardial infarction

KW - Reperfusion injury

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