H1foo Has a Pivotal Role in Qualifying Induced Pluripotent Stem Cells

Akira Kunitomi, Shinsuke Yuasa, Fumihiro Sugiyama, Yuki Saito, Tomohisa Seki, Dai Kusumoto, Shin Kashimura, Makoto Takei, Shugo Tohyama, Hisayuki Hashimoto, Toru Egashira, Yoko Tanimoto, Saori Mizuno, Shoma Tanaka, Hironobu Okuno, Kazuki Yamazawa, Hideo Watanabe, Mayumi Oda, Ruri Kaneda, Yumi MatsuzakiToshihiro Nagai, Hideyuki Okano, Ken ichi Yagami, Mamoru Tanaka, Keiichi Fukuda

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Embryonic stem cells (ESCs) are a hallmark of ideal pluripotent stem cells. Epigenetic reprogramming of induced pluripotent stem cells (iPSCs) has not been fully accomplished. iPSC generation is similar to somatic cell nuclear transfer (SCNT) in oocytes, and this procedure can be used to generate ESCs (SCNT-ESCs), which suggests the contribution of oocyte-specific constituents. Here, we show that the mammalian oocyte-specific linker histone H1foo has beneficial effects on iPSC generation. Induction of H1foo with Oct4, Sox2, and Klf4 significantly enhanced the efficiency of iPSC generation. H1foo promoted in vitro differentiation characteristics with low heterogeneity in iPSCs. H1foo enhanced the generation of germline-competent chimeric mice from iPSCs in a manner similar to that for ESCs. These findings indicate that H1foo contributes to the generation of higher-quality iPSCs. In this article, Yuasa and colleagues show that the mammalian oocyte-specific linker histone H1foo has beneficial effects on iPSC generation. Induction of H1foo with Oct4, Sox2, and Klf4 enhanced the efficiency of iPSC generation and improved the quality of iPSCs with low heterogeneity in vitro and in vivo. These findings indicate that H1foo contributes to the generation of higher quality iPSCs.

Original languageEnglish
JournalStem Cell Reports
DOIs
Publication statusAccepted/In press - 2015 Apr 28

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Induced Pluripotent Stem Cells
Stem cells
Embryonic Stem Cells
Oocytes
Histones
Pluripotent Stem Cells
Epigenomics

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Developmental Biology
  • Genetics

Cite this

H1foo Has a Pivotal Role in Qualifying Induced Pluripotent Stem Cells. / Kunitomi, Akira; Yuasa, Shinsuke; Sugiyama, Fumihiro; Saito, Yuki; Seki, Tomohisa; Kusumoto, Dai; Kashimura, Shin; Takei, Makoto; Tohyama, Shugo; Hashimoto, Hisayuki; Egashira, Toru; Tanimoto, Yoko; Mizuno, Saori; Tanaka, Shoma; Okuno, Hironobu; Yamazawa, Kazuki; Watanabe, Hideo; Oda, Mayumi; Kaneda, Ruri; Matsuzaki, Yumi; Nagai, Toshihiro; Okano, Hideyuki; Yagami, Ken ichi; Tanaka, Mamoru; Fukuda, Keiichi.

In: Stem Cell Reports, 28.04.2015.

Research output: Contribution to journalArticle

Kunitomi, A, Yuasa, S, Sugiyama, F, Saito, Y, Seki, T, Kusumoto, D, Kashimura, S, Takei, M, Tohyama, S, Hashimoto, H, Egashira, T, Tanimoto, Y, Mizuno, S, Tanaka, S, Okuno, H, Yamazawa, K, Watanabe, H, Oda, M, Kaneda, R, Matsuzaki, Y, Nagai, T, Okano, H, Yagami, KI, Tanaka, M & Fukuda, K 2015, 'H1foo Has a Pivotal Role in Qualifying Induced Pluripotent Stem Cells', Stem Cell Reports. https://doi.org/10.1016/j.stemcr.2016.04.015
Kunitomi, Akira ; Yuasa, Shinsuke ; Sugiyama, Fumihiro ; Saito, Yuki ; Seki, Tomohisa ; Kusumoto, Dai ; Kashimura, Shin ; Takei, Makoto ; Tohyama, Shugo ; Hashimoto, Hisayuki ; Egashira, Toru ; Tanimoto, Yoko ; Mizuno, Saori ; Tanaka, Shoma ; Okuno, Hironobu ; Yamazawa, Kazuki ; Watanabe, Hideo ; Oda, Mayumi ; Kaneda, Ruri ; Matsuzaki, Yumi ; Nagai, Toshihiro ; Okano, Hideyuki ; Yagami, Ken ichi ; Tanaka, Mamoru ; Fukuda, Keiichi. / H1foo Has a Pivotal Role in Qualifying Induced Pluripotent Stem Cells. In: Stem Cell Reports. 2015.
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