Handheld nanopore-based biosensing device

Yusuke Izawa; Toshihisa Osaki; Koki Kamiya; Satoshi Fujii; Nobuo Misawa; Norihisa Miki; Shoji Takeuchi

doi:10.1109/MEMSYS.2018.8346466

Handheld nanopore-based biosensing device

Yusuke Izawa, Toshihisa Osaki, Koki Kamiya, Satoshi Fujii, Nobuo Misawa, Norihisa Miki, Shoji Takeuchi

Department of Mechanical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

This paper describes the development of a handheld device for long-term nanopore-based biosensing. Recently, membrane protein reconstituted in bilayer lipid membrane (BLM) has been applied for bio/chemical sensors because membrane protein intrinsically has a superior amplification mechanism. For mobile use of such devices, however, the bilayer requires mechanical robustness. Here, we propose a handheld BLM device aiming for a nanopore-based mobile sensor. BLM is formed at the tip of a glass capillary, which is contained in the solution trapped in a cup-like reservoir. This format achieved (i) formation of a sufficiently robust BLM for mobile use, (ii) solution exchange without rupturing BLM, and (iii) sustaining the small number of nanopores in the bilayer for accurate and long-term sensing. The handheld device successfully detected DNA translocations through the nanopores continuously for 75 minutes using 5 nanopores.

Original language	English
Title of host publication	2018 IEEE Micro Electro Mechanical Systems, MEMS 2018
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	2-5
Number of pages	4
Volume	2018-January
ISBN (Electronic)	9781538647820
DOIs	https://doi.org/10.1109/MEMSYS.2018.8346466
Publication status	Published - 2018 Apr 24
Event	31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018 - Belfast, United Kingdom Duration: 2018 Jan 21 → 2018 Jan 25

Other

Other	31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018
Country	United Kingdom
City	Belfast
Period	18/1/21 → 18/1/25

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/MEMSYS.2018.8346466

Fingerprint Dive into the research topics of 'Handheld nanopore-based biosensing device'. Together they form a unique fingerprint.

Cite this

Handheld nanopore-based biosensing device. / Izawa, Yusuke; Osaki, Toshihisa; Kamiya, Koki; Fujii, Satoshi; Misawa, Nobuo; Miki, Norihisa; Takeuchi, Shoji.

2018 IEEE Micro Electro Mechanical Systems, MEMS 2018. Vol. 2018-January Institute of Electrical and Electronics Engineers Inc., 2018. p. 2-5.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Izawa, Y, Osaki, T, Kamiya, K, Fujii, S, Misawa, N, Miki, N & Takeuchi, S 2018, Handheld nanopore-based biosensing device. in 2018 IEEE Micro Electro Mechanical Systems, MEMS 2018. vol. 2018-January, Institute of Electrical and Electronics Engineers Inc., pp. 2-5, 31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018, Belfast, United Kingdom, 18/1/21. https://doi.org/10.1109/MEMSYS.2018.8346466

@inproceedings{dc49711764af4231abdf296c08938b56,

title = "Handheld nanopore-based biosensing device",

abstract = "This paper describes the development of a handheld device for long-term nanopore-based biosensing. Recently, membrane protein reconstituted in bilayer lipid membrane (BLM) has been applied for bio/chemical sensors because membrane protein intrinsically has a superior amplification mechanism. For mobile use of such devices, however, the bilayer requires mechanical robustness. Here, we propose a handheld BLM device aiming for a nanopore-based mobile sensor. BLM is formed at the tip of a glass capillary, which is contained in the solution trapped in a cup-like reservoir. This format achieved (i) formation of a sufficiently robust BLM for mobile use, (ii) solution exchange without rupturing BLM, and (iii) sustaining the small number of nanopores in the bilayer for accurate and long-term sensing. The handheld device successfully detected DNA translocations through the nanopores continuously for 75 minutes using 5 nanopores.",

author = "Yusuke Izawa and Toshihisa Osaki and Koki Kamiya and Satoshi Fujii and Nobuo Misawa and Norihisa Miki and Shoji Takeuchi",

year = "2018",

month = apr,

day = "24",

doi = "10.1109/MEMSYS.2018.8346466",

language = "English",

volume = "2018-January",

pages = "2--5",

booktitle = "2018 IEEE Micro Electro Mechanical Systems, MEMS 2018",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

note = "31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018 ; Conference date: 21-01-2018 Through 25-01-2018",

}

TY - GEN

T1 - Handheld nanopore-based biosensing device

AU - Izawa, Yusuke

AU - Osaki, Toshihisa

AU - Kamiya, Koki

AU - Fujii, Satoshi

AU - Misawa, Nobuo

AU - Miki, Norihisa

AU - Takeuchi, Shoji

PY - 2018/4/24

Y1 - 2018/4/24

N2 - This paper describes the development of a handheld device for long-term nanopore-based biosensing. Recently, membrane protein reconstituted in bilayer lipid membrane (BLM) has been applied for bio/chemical sensors because membrane protein intrinsically has a superior amplification mechanism. For mobile use of such devices, however, the bilayer requires mechanical robustness. Here, we propose a handheld BLM device aiming for a nanopore-based mobile sensor. BLM is formed at the tip of a glass capillary, which is contained in the solution trapped in a cup-like reservoir. This format achieved (i) formation of a sufficiently robust BLM for mobile use, (ii) solution exchange without rupturing BLM, and (iii) sustaining the small number of nanopores in the bilayer for accurate and long-term sensing. The handheld device successfully detected DNA translocations through the nanopores continuously for 75 minutes using 5 nanopores.

AB - This paper describes the development of a handheld device for long-term nanopore-based biosensing. Recently, membrane protein reconstituted in bilayer lipid membrane (BLM) has been applied for bio/chemical sensors because membrane protein intrinsically has a superior amplification mechanism. For mobile use of such devices, however, the bilayer requires mechanical robustness. Here, we propose a handheld BLM device aiming for a nanopore-based mobile sensor. BLM is formed at the tip of a glass capillary, which is contained in the solution trapped in a cup-like reservoir. This format achieved (i) formation of a sufficiently robust BLM for mobile use, (ii) solution exchange without rupturing BLM, and (iii) sustaining the small number of nanopores in the bilayer for accurate and long-term sensing. The handheld device successfully detected DNA translocations through the nanopores continuously for 75 minutes using 5 nanopores.

UR - http://www.scopus.com/inward/record.url?scp=85046997151&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85046997151&partnerID=8YFLogxK

U2 - 10.1109/MEMSYS.2018.8346466

DO - 10.1109/MEMSYS.2018.8346466

M3 - Conference contribution

AN - SCOPUS:85046997151

VL - 2018-January

SP - 2

EP - 5

BT - 2018 IEEE Micro Electro Mechanical Systems, MEMS 2018

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018

Y2 - 21 January 2018 through 25 January 2018

ER -