Handheld nanopore-based biosensing device

Yusuke Izawa; Toshihisa Osaki; Koki Kamiya; Satoshi Fujii; Nobuo Misawa; Norihisa Miki; Shoji Takeuchi

doi:10.1109/MEMSYS.2018.8346466

Handheld nanopore-based biosensing device

Yusuke Izawa, Toshihisa Osaki, Koki Kamiya, Satoshi Fujii, Nobuo Misawa, Norihisa Miki, Shoji Takeuchi

Department of Mechanical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

This paper describes the development of a handheld device for long-term nanopore-based biosensing. Recently, membrane protein reconstituted in bilayer lipid membrane (BLM) has been applied for bio/chemical sensors because membrane protein intrinsically has a superior amplification mechanism. For mobile use of such devices, however, the bilayer requires mechanical robustness. Here, we propose a handheld BLM device aiming for a nanopore-based mobile sensor. BLM is formed at the tip of a glass capillary, which is contained in the solution trapped in a cup-like reservoir. This format achieved (i) formation of a sufficiently robust BLM for mobile use, (ii) solution exchange without rupturing BLM, and (iii) sustaining the small number of nanopores in the bilayer for accurate and long-term sensing. The handheld device successfully detected DNA translocations through the nanopores continuously for 75 minutes using 5 nanopores.

Original language	English
Title of host publication	2018 IEEE Micro Electro Mechanical Systems, MEMS 2018
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	2-5
Number of pages	4
Volume	2018-January
ISBN (Electronic)	9781538647820
DOIs	https://doi.org/10.1109/MEMSYS.2018.8346466
Publication status	Published - 2018 Apr 24
Event	31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018 - Belfast, United Kingdom Duration: 2018 Jan 21 → 2018 Jan 25

Other

Other	31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018
Country	United Kingdom
City	Belfast
Period	18/1/21 → 18/1/25

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

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Cite this

Izawa, Y., Osaki, T., Kamiya, K., Fujii, S., Misawa, N., Miki, N., & Takeuchi, S. (2018). Handheld nanopore-based biosensing device. In 2018 IEEE Micro Electro Mechanical Systems, MEMS 2018 (Vol. 2018-January, pp. 2-5). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2018.8346466

Handheld nanopore-based biosensing device. / Izawa, Yusuke; Osaki, Toshihisa; Kamiya, Koki; Fujii, Satoshi; Misawa, Nobuo; Miki, Norihisa; Takeuchi, Shoji.

2018 IEEE Micro Electro Mechanical Systems, MEMS 2018. Vol. 2018-January Institute of Electrical and Electronics Engineers Inc., 2018. p. 2-5.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Izawa, Y, Osaki, T, Kamiya, K, Fujii, S, Misawa, N, Miki, N & Takeuchi, S 2018, Handheld nanopore-based biosensing device. in 2018 IEEE Micro Electro Mechanical Systems, MEMS 2018. vol. 2018-January, Institute of Electrical and Electronics Engineers Inc., pp. 2-5, 31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018, Belfast, United Kingdom, 18/1/21. https://doi.org/10.1109/MEMSYS.2018.8346466

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