Handheld nanopore-based biosensing device

Yusuke Izawa, Toshihisa Osaki, Koki Kamiya, Satoshi Fujii, Nobuo Misawa, Norihisa Miki, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper describes the development of a handheld device for long-term nanopore-based biosensing. Recently, membrane protein reconstituted in bilayer lipid membrane (BLM) has been applied for bio/chemical sensors because membrane protein intrinsically has a superior amplification mechanism. For mobile use of such devices, however, the bilayer requires mechanical robustness. Here, we propose a handheld BLM device aiming for a nanopore-based mobile sensor. BLM is formed at the tip of a glass capillary, which is contained in the solution trapped in a cup-like reservoir. This format achieved (i) formation of a sufficiently robust BLM for mobile use, (ii) solution exchange without rupturing BLM, and (iii) sustaining the small number of nanopores in the bilayer for accurate and long-term sensing. The handheld device successfully detected DNA translocations through the nanopores continuously for 75 minutes using 5 nanopores.

Original languageEnglish
Title of host publication2018 IEEE Micro Electro Mechanical Systems, MEMS 2018
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages2-5
Number of pages4
Volume2018-January
ISBN (Electronic)9781538647820
DOIs
Publication statusPublished - 2018 Apr 24
Event31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018 - Belfast, United Kingdom
Duration: 2018 Jan 212018 Jan 25

Other

Other31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018
CountryUnited Kingdom
CityBelfast
Period18/1/2118/1/25

Fingerprint

Lipid bilayers
Nanopores
Membrane Lipids
membranes
lipids
Membrane Proteins
Proteins
Membranes
Chemical sensors
rupturing
proteins
Amplification
Ion exchange
sustaining
sensors
DNA
format
Glass
deoxyribonucleic acid
Sensors

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Izawa, Y., Osaki, T., Kamiya, K., Fujii, S., Misawa, N., Miki, N., & Takeuchi, S. (2018). Handheld nanopore-based biosensing device. In 2018 IEEE Micro Electro Mechanical Systems, MEMS 2018 (Vol. 2018-January, pp. 2-5). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2018.8346466

Handheld nanopore-based biosensing device. / Izawa, Yusuke; Osaki, Toshihisa; Kamiya, Koki; Fujii, Satoshi; Misawa, Nobuo; Miki, Norihisa; Takeuchi, Shoji.

2018 IEEE Micro Electro Mechanical Systems, MEMS 2018. Vol. 2018-January Institute of Electrical and Electronics Engineers Inc., 2018. p. 2-5.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Izawa, Y, Osaki, T, Kamiya, K, Fujii, S, Misawa, N, Miki, N & Takeuchi, S 2018, Handheld nanopore-based biosensing device. in 2018 IEEE Micro Electro Mechanical Systems, MEMS 2018. vol. 2018-January, Institute of Electrical and Electronics Engineers Inc., pp. 2-5, 31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018, Belfast, United Kingdom, 18/1/21. https://doi.org/10.1109/MEMSYS.2018.8346466
Izawa Y, Osaki T, Kamiya K, Fujii S, Misawa N, Miki N et al. Handheld nanopore-based biosensing device. In 2018 IEEE Micro Electro Mechanical Systems, MEMS 2018. Vol. 2018-January. Institute of Electrical and Electronics Engineers Inc. 2018. p. 2-5 https://doi.org/10.1109/MEMSYS.2018.8346466
Izawa, Yusuke ; Osaki, Toshihisa ; Kamiya, Koki ; Fujii, Satoshi ; Misawa, Nobuo ; Miki, Norihisa ; Takeuchi, Shoji. / Handheld nanopore-based biosensing device. 2018 IEEE Micro Electro Mechanical Systems, MEMS 2018. Vol. 2018-January Institute of Electrical and Electronics Engineers Inc., 2018. pp. 2-5
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