Abstract
This paper describes the development of a handheld device for long-term nanopore-based biosensing. Recently, membrane protein reconstituted in bilayer lipid membrane (BLM) has been applied for bio/chemical sensors because membrane protein intrinsically has a superior amplification mechanism. For mobile use of such devices, however, the bilayer requires mechanical robustness. Here, we propose a handheld BLM device aiming for a nanopore-based mobile sensor. BLM is formed at the tip of a glass capillary, which is contained in the solution trapped in a cup-like reservoir. This format achieved (i) formation of a sufficiently robust BLM for mobile use, (ii) solution exchange without rupturing BLM, and (iii) sustaining the small number of nanopores in the bilayer for accurate and long-term sensing. The handheld device successfully detected DNA translocations through the nanopores continuously for 75 minutes using 5 nanopores.
Original language | English |
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Title of host publication | 2018 IEEE Micro Electro Mechanical Systems, MEMS 2018 |
Publisher | Institute of Electrical and Electronics Engineers Inc. |
Pages | 2-5 |
Number of pages | 4 |
Volume | 2018-January |
ISBN (Electronic) | 9781538647820 |
DOIs | |
Publication status | Published - 2018 Apr 24 |
Event | 31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018 - Belfast, United Kingdom Duration: 2018 Jan 21 → 2018 Jan 25 |
Other
Other | 31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018 |
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Country | United Kingdom |
City | Belfast |
Period | 18/1/21 → 18/1/25 |
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Mechanical Engineering
- Electrical and Electronic Engineering