Abstract
This paper describes the development of a handheld device for long-term nanopore-based biosensing. Recently, membrane protein reconstituted in bilayer lipid membrane (BLM) has been applied for bio/chemical sensors because membrane protein intrinsically has a superior amplification mechanism. For mobile use of such devices, however, the bilayer requires mechanical robustness. Here, we propose a handheld BLM device aiming for a nanopore-based mobile sensor. BLM is formed at the tip of a glass capillary, which is contained in the solution trapped in a cup-like reservoir. This format achieved (i) formation of a sufficiently robust BLM for mobile use, (ii) solution exchange without rupturing BLM, and (iii) sustaining the small number of nanopores in the bilayer for accurate and long-term sensing. The handheld device successfully detected DNA translocations through the nanopores continuously for 75 minutes using 5 nanopores.
| Original language | English |
|---|---|
| Title of host publication | 2018 IEEE Micro Electro Mechanical Systems, MEMS 2018 |
| Publisher | Institute of Electrical and Electronics Engineers Inc. |
| Pages | 2-5 |
| Number of pages | 4 |
| Volume | 2018-January |
| ISBN (Electronic) | 9781538647820 |
| DOIs | |
| Publication status | Published - 2018 Apr 24 |
| Event | 31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018 - Belfast, United Kingdom Duration: 2018 Jan 21 → 2018 Jan 25 |
Other
| Other | 31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018 |
|---|---|
| Country | United Kingdom |
| City | Belfast |
| Period | 18/1/21 → 18/1/25 |
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ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Mechanical Engineering
- Electrical and Electronic Engineering
Cite this
Handheld nanopore-based biosensing device. / Izawa, Yusuke; Osaki, Toshihisa; Kamiya, Koki; Fujii, Satoshi; Misawa, Nobuo; Miki, Norihisa; Takeuchi, Shoji.
2018 IEEE Micro Electro Mechanical Systems, MEMS 2018. Vol. 2018-January Institute of Electrical and Electronics Engineers Inc., 2018. p. 2-5.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Handheld nanopore-based biosensing device
AU - Izawa, Yusuke
AU - Osaki, Toshihisa
AU - Kamiya, Koki
AU - Fujii, Satoshi
AU - Misawa, Nobuo
AU - Miki, Norihisa
AU - Takeuchi, Shoji
PY - 2018/4/24
Y1 - 2018/4/24
N2 - This paper describes the development of a handheld device for long-term nanopore-based biosensing. Recently, membrane protein reconstituted in bilayer lipid membrane (BLM) has been applied for bio/chemical sensors because membrane protein intrinsically has a superior amplification mechanism. For mobile use of such devices, however, the bilayer requires mechanical robustness. Here, we propose a handheld BLM device aiming for a nanopore-based mobile sensor. BLM is formed at the tip of a glass capillary, which is contained in the solution trapped in a cup-like reservoir. This format achieved (i) formation of a sufficiently robust BLM for mobile use, (ii) solution exchange without rupturing BLM, and (iii) sustaining the small number of nanopores in the bilayer for accurate and long-term sensing. The handheld device successfully detected DNA translocations through the nanopores continuously for 75 minutes using 5 nanopores.
AB - This paper describes the development of a handheld device for long-term nanopore-based biosensing. Recently, membrane protein reconstituted in bilayer lipid membrane (BLM) has been applied for bio/chemical sensors because membrane protein intrinsically has a superior amplification mechanism. For mobile use of such devices, however, the bilayer requires mechanical robustness. Here, we propose a handheld BLM device aiming for a nanopore-based mobile sensor. BLM is formed at the tip of a glass capillary, which is contained in the solution trapped in a cup-like reservoir. This format achieved (i) formation of a sufficiently robust BLM for mobile use, (ii) solution exchange without rupturing BLM, and (iii) sustaining the small number of nanopores in the bilayer for accurate and long-term sensing. The handheld device successfully detected DNA translocations through the nanopores continuously for 75 minutes using 5 nanopores.
UR - http://www.scopus.com/inward/record.url?scp=85046997151&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85046997151&partnerID=8YFLogxK
U2 - 10.1109/MEMSYS.2018.8346466
DO - 10.1109/MEMSYS.2018.8346466
M3 - Conference contribution
AN - SCOPUS:85046997151
VL - 2018-January
SP - 2
EP - 5
BT - 2018 IEEE Micro Electro Mechanical Systems, MEMS 2018
PB - Institute of Electrical and Electronics Engineers Inc.
ER -