Haploid specific activations of protamine 1 and hsc70t genes in mouse spermatogenesis

Midori Matsumoto, Shun ichi Kurata, Hirokazu Fujimoto, Motonori Hoshi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Protamine 1 and heat shock cognate 70 kDa protein (hsc70t) are known to be synthesized in haploid cells during spermatogenesis, and the mRNAs of these proteins have also been shown to accumulate in the haploid cells. However, it is unknown at which stage of spermatogenesis the genes for these proteins are actually activated. To examine this problem, we fractionated mouse adult testes cells at four different developmental stages, extracted their nuclei and carried out run-off assays with hsc70t and protamine 1 DNA probes. Results showed that both genes are mainly activated at the round spermatid stage. As the protein products of these genes accumulate at the later stage, it is interesting that these genes are regulated at the transcriptional and translational levels during spermatogenesis.

Original languageEnglish
Pages (from-to)274-278
Number of pages5
JournalBBA - Gene Structure and Expression
Volume1174
Issue number3
DOIs
Publication statusPublished - 1993 Sep 23
Externally publishedYes

Fingerprint

HSC70 Heat-Shock Proteins
Protamines
Haploidy
Spermatogenesis
Genes
Chemical activation
Proteins
Spermatids
DNA Probes
Testis
Assays
Messenger RNA

Keywords

  • (Mouse)
  • Gene activation
  • Heat shock cognate protein
  • mRNA
  • Spermatogenesis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology

Cite this

Haploid specific activations of protamine 1 and hsc70t genes in mouse spermatogenesis. / Matsumoto, Midori; Kurata, Shun ichi; Fujimoto, Hirokazu; Hoshi, Motonori.

In: BBA - Gene Structure and Expression, Vol. 1174, No. 3, 23.09.1993, p. 274-278.

Research output: Contribution to journalArticle

Matsumoto, Midori ; Kurata, Shun ichi ; Fujimoto, Hirokazu ; Hoshi, Motonori. / Haploid specific activations of protamine 1 and hsc70t genes in mouse spermatogenesis. In: BBA - Gene Structure and Expression. 1993 ; Vol. 1174, No. 3. pp. 274-278.
@article{351faa795d5e4aa8ba62fd0e131aa6cd,
title = "Haploid specific activations of protamine 1 and hsc70t genes in mouse spermatogenesis",
abstract = "Protamine 1 and heat shock cognate 70 kDa protein (hsc70t) are known to be synthesized in haploid cells during spermatogenesis, and the mRNAs of these proteins have also been shown to accumulate in the haploid cells. However, it is unknown at which stage of spermatogenesis the genes for these proteins are actually activated. To examine this problem, we fractionated mouse adult testes cells at four different developmental stages, extracted their nuclei and carried out run-off assays with hsc70t and protamine 1 DNA probes. Results showed that both genes are mainly activated at the round spermatid stage. As the protein products of these genes accumulate at the later stage, it is interesting that these genes are regulated at the transcriptional and translational levels during spermatogenesis.",
keywords = "(Mouse), Gene activation, Heat shock cognate protein, mRNA, Spermatogenesis",
author = "Midori Matsumoto and Kurata, {Shun ichi} and Hirokazu Fujimoto and Motonori Hoshi",
year = "1993",
month = "9",
day = "23",
doi = "10.1016/0167-4781(93)90196-K",
language = "English",
volume = "1174",
pages = "274--278",
journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",
issn = "0167-4781",
publisher = "Elsevier BV",
number = "3",

}

TY - JOUR

T1 - Haploid specific activations of protamine 1 and hsc70t genes in mouse spermatogenesis

AU - Matsumoto, Midori

AU - Kurata, Shun ichi

AU - Fujimoto, Hirokazu

AU - Hoshi, Motonori

PY - 1993/9/23

Y1 - 1993/9/23

N2 - Protamine 1 and heat shock cognate 70 kDa protein (hsc70t) are known to be synthesized in haploid cells during spermatogenesis, and the mRNAs of these proteins have also been shown to accumulate in the haploid cells. However, it is unknown at which stage of spermatogenesis the genes for these proteins are actually activated. To examine this problem, we fractionated mouse adult testes cells at four different developmental stages, extracted their nuclei and carried out run-off assays with hsc70t and protamine 1 DNA probes. Results showed that both genes are mainly activated at the round spermatid stage. As the protein products of these genes accumulate at the later stage, it is interesting that these genes are regulated at the transcriptional and translational levels during spermatogenesis.

AB - Protamine 1 and heat shock cognate 70 kDa protein (hsc70t) are known to be synthesized in haploid cells during spermatogenesis, and the mRNAs of these proteins have also been shown to accumulate in the haploid cells. However, it is unknown at which stage of spermatogenesis the genes for these proteins are actually activated. To examine this problem, we fractionated mouse adult testes cells at four different developmental stages, extracted their nuclei and carried out run-off assays with hsc70t and protamine 1 DNA probes. Results showed that both genes are mainly activated at the round spermatid stage. As the protein products of these genes accumulate at the later stage, it is interesting that these genes are regulated at the transcriptional and translational levels during spermatogenesis.

KW - (Mouse)

KW - Gene activation

KW - Heat shock cognate protein

KW - mRNA

KW - Spermatogenesis

UR - http://www.scopus.com/inward/record.url?scp=0027328770&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027328770&partnerID=8YFLogxK

U2 - 10.1016/0167-4781(93)90196-K

DO - 10.1016/0167-4781(93)90196-K

M3 - Article

C2 - 8373804

AN - SCOPUS:0027328770

VL - 1174

SP - 274

EP - 278

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

SN - 0167-4781

IS - 3

ER -