Hard contact lens-induced metabolic changes in rabbit corneas

Kazuo Tsubota, Kenneth R. Kenyon, Hong Ming Cheng

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The biochemistry of contact lens-cornea interaction is not well understood, although previous studies have suggested that corneal metabolic changes may be the underlying factor in morphological alterations. Using a rabbit model, this interaction has been examined with 31P nuclear magnetic resonance (NMR) spectroscopy, which detects signals principally from the epithelium. The examination was supplemented with electron microscopy and histochemistry. Polymethylmethacrylate lenses caused reversible changes, including activation of anaerobic glycolysis and disturbance of membrane metabolite levels. These changes were far more severe than those occurring during prolonged eye closure. There appears to be an association between cellular deterioration and loss of membrane metabolites. On the other hand, oxygen-permeable silicone lenses allowed maintenance of nearly normal metabolic patterns. These results show multifaceted corneal response to hard contact lens wear.

Original languageEnglish
Pages (from-to)769-775
Number of pages7
JournalExperimental Eye Research
Volume49
Issue number5
DOIs
Publication statusPublished - 1989
Externally publishedYes

Fingerprint

Contact Lenses
Cornea
Lenses
Rabbits
Membranes
Polymethyl Methacrylate
Glycolysis
Silicones
Biochemistry
Electron Microscopy
Magnetic Resonance Spectroscopy
Epithelium
Maintenance
Oxygen

Keywords

  • contact lens
  • cornea
  • Dk
  • glycolysis
  • membrane metabolism
  • NMR

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Hard contact lens-induced metabolic changes in rabbit corneas. / Tsubota, Kazuo; Kenyon, Kenneth R.; Cheng, Hong Ming.

In: Experimental Eye Research, Vol. 49, No. 5, 1989, p. 769-775.

Research output: Contribution to journalArticle

Tsubota, Kazuo ; Kenyon, Kenneth R. ; Cheng, Hong Ming. / Hard contact lens-induced metabolic changes in rabbit corneas. In: Experimental Eye Research. 1989 ; Vol. 49, No. 5. pp. 769-775.
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