Heart regeneration using reprogramming technology

Masaki Ieda

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Loss of terminally differentiated cardiomyocytes due to heart disease is irreversible and current therapeutic regimes are limited. Cell therapy using stem cell-derived cardiomyocytes is an attractive option to repair injured hearts. The discovery of direct reprogramming of broblasts into induced pluripotent stem cells (iPSCs) and successful differentiation of iPSCs into cardiomyocytes provided a revolutionary paradigm in heart regenerative research. During the past decades, signicant advances in stem cell culture, differentiation and purication protocols, as well as in cell transplantation methodologies, have been achieved. On the other hand, recent studies demonstrated that a somatic cell could be converted into an alternative differentiated cell type without rst becoming a stem cell by overexpression of lineage-specic factors. We found that functional cardiomyocytes can be directly induced from broblasts by a combination of three cardiac transcription factors, Gata4, Mef2c and Tbx5, in vitro and in vivo. I will review the perspectives of heart regeneration using reprogramming technology.

Original languageEnglish
Pages (from-to)118-128
Number of pages11
JournalProceedings of the Japan Academy Series B: Physical and Biological Sciences
Volume89
Issue number3
DOIs
Publication statusPublished - 2013

Fingerprint

stem cells
regeneration
Cardiac Myocytes
Regeneration
heart
Technology
Induced Pluripotent Stem Cells
Stem Cells
Cell Differentiation
cells
therapeutics
cell transplantation
Cell Transplantation
heart diseases
Cell Lineage
Cell- and Tissue-Based Therapy
transplantation
somatic cells
Heart Diseases
cell culture

Keywords

  • Heart
  • Heart regeneration
  • Induced cardiomyocyte
  • IPS cell
  • Reprogramming

ASJC Scopus subject areas

  • General

Cite this

Heart regeneration using reprogramming technology. / Ieda, Masaki.

In: Proceedings of the Japan Academy Series B: Physical and Biological Sciences, Vol. 89, No. 3, 2013, p. 118-128.

Research output: Contribution to journalArticle

@article{18b21e69f37243938676695ef20e82a7,
title = "Heart regeneration using reprogramming technology",
abstract = "Loss of terminally differentiated cardiomyocytes due to heart disease is irreversible and current therapeutic regimes are limited. Cell therapy using stem cell-derived cardiomyocytes is an attractive option to repair injured hearts. The discovery of direct reprogramming of broblasts into induced pluripotent stem cells (iPSCs) and successful differentiation of iPSCs into cardiomyocytes provided a revolutionary paradigm in heart regenerative research. During the past decades, signicant advances in stem cell culture, differentiation and purication protocols, as well as in cell transplantation methodologies, have been achieved. On the other hand, recent studies demonstrated that a somatic cell could be converted into an alternative differentiated cell type without rst becoming a stem cell by overexpression of lineage-specic factors. We found that functional cardiomyocytes can be directly induced from broblasts by a combination of three cardiac transcription factors, Gata4, Mef2c and Tbx5, in vitro and in vivo. I will review the perspectives of heart regeneration using reprogramming technology.",
keywords = "Heart, Heart regeneration, Induced cardiomyocyte, IPS cell, Reprogramming",
author = "Masaki Ieda",
year = "2013",
doi = "10.2183/pjab.89.118",
language = "English",
volume = "89",
pages = "118--128",
journal = "Proceedings of the Japan Academy Series B: Physical and Biological Sciences",
issn = "0386-2208",
publisher = "Japan Academy",
number = "3",

}

TY - JOUR

T1 - Heart regeneration using reprogramming technology

AU - Ieda, Masaki

PY - 2013

Y1 - 2013

N2 - Loss of terminally differentiated cardiomyocytes due to heart disease is irreversible and current therapeutic regimes are limited. Cell therapy using stem cell-derived cardiomyocytes is an attractive option to repair injured hearts. The discovery of direct reprogramming of broblasts into induced pluripotent stem cells (iPSCs) and successful differentiation of iPSCs into cardiomyocytes provided a revolutionary paradigm in heart regenerative research. During the past decades, signicant advances in stem cell culture, differentiation and purication protocols, as well as in cell transplantation methodologies, have been achieved. On the other hand, recent studies demonstrated that a somatic cell could be converted into an alternative differentiated cell type without rst becoming a stem cell by overexpression of lineage-specic factors. We found that functional cardiomyocytes can be directly induced from broblasts by a combination of three cardiac transcription factors, Gata4, Mef2c and Tbx5, in vitro and in vivo. I will review the perspectives of heart regeneration using reprogramming technology.

AB - Loss of terminally differentiated cardiomyocytes due to heart disease is irreversible and current therapeutic regimes are limited. Cell therapy using stem cell-derived cardiomyocytes is an attractive option to repair injured hearts. The discovery of direct reprogramming of broblasts into induced pluripotent stem cells (iPSCs) and successful differentiation of iPSCs into cardiomyocytes provided a revolutionary paradigm in heart regenerative research. During the past decades, signicant advances in stem cell culture, differentiation and purication protocols, as well as in cell transplantation methodologies, have been achieved. On the other hand, recent studies demonstrated that a somatic cell could be converted into an alternative differentiated cell type without rst becoming a stem cell by overexpression of lineage-specic factors. We found that functional cardiomyocytes can be directly induced from broblasts by a combination of three cardiac transcription factors, Gata4, Mef2c and Tbx5, in vitro and in vivo. I will review the perspectives of heart regeneration using reprogramming technology.

KW - Heart

KW - Heart regeneration

KW - Induced cardiomyocyte

KW - IPS cell

KW - Reprogramming

UR - http://www.scopus.com/inward/record.url?scp=84875773584&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875773584&partnerID=8YFLogxK

U2 - 10.2183/pjab.89.118

DO - 10.2183/pjab.89.118

M3 - Article

C2 - 23474887

AN - SCOPUS:84875773584

VL - 89

SP - 118

EP - 128

JO - Proceedings of the Japan Academy Series B: Physical and Biological Sciences

JF - Proceedings of the Japan Academy Series B: Physical and Biological Sciences

SN - 0386-2208

IS - 3

ER -