Heat enhances Ex vivo paclitaxel delivery to porcine carotid artery wall

Rie Homma, Machiko Shinozuka, Natsumi Shimazaki, Emiyu Ogawa, Tsunenori Arai

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We investigated the delivery of rhodamine B and Oregon Green®-labeled paclitaxel (OGLP) in ex vivo porcine carotid artery wall (CAW) samples after heating the reagents to 50–70°C for 15 s. When the isolated CAW samples were placed in the heated fluorophore solutions, the penetration depth of the hydrophobic rhodamine B increased significantly compared with reference solution at 37°C. The penetration depth of OGLP also tended to increase upon heating to 70°C for 15 s. We also studied the mechanism of this agent delivery enhancement by observing the inner surface structure and hydrophobicity of the CAW samples after heating. An expanded mesh structure at the inner surface of the heated CAW samples was observed upon heating above 70°C, and the mean hydrophobicity of the media layer also increased significantly. We hypothesize that heating at 60–70°C for 15 s enhances the delivery of fluorophores to CAW samples as a result of an expanded mesh structure at the inner surface of the CAW, along with a simultaneous increase in hydrophobicity.

Original languageEnglish
Pages (from-to)30-37
Number of pages8
JournalAdvanced Biomedical Engineering
Volume8
DOIs
Publication statusPublished - 2019 Jan 1

Keywords

  • Drug delivery
  • Heating
  • Paclitaxel
  • Porcine carotid artery wall

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Computer Science Applications

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