TY - JOUR
T1 - Heat shock-induced DNA relaxation in vitro by DNA gyrase of Escherichia coli in the presence of ATP
AU - Kataoka, Kazuhiro
AU - Mizushima, Tohru
AU - Ogata, Yasuyuki
AU - Miki, Takeyoshi
AU - Sekimizu, Kazuhisa
PY - 1996
Y1 - 1996
N2 - Genetic studies revealed that DNA gyrase seems to catalyze immediate and transient DNA relaxation after Escherichia coli cells are exposed to heat shock (Ogata, Y., Mizushima, T., Kataoka, K., Miki, T., and Sekimizu, K. (1994) Mol. Gen. Genet. 244, 451-455). We have now obtained biochemical evidence to support this hypothesis. DNA gyrase catalyzed an increase in the linking number of DNA and relaxation of negatively supercoiled DNA, under physiological concentrations of ATP. Analyses by gel filtration chromatography of each subunit revealed that DNA relaxation activity co- migrated with each subunit. The linking number of DNA increased as the temperature increased. Further, the reaction was inhibited by nalidixic acid or by oxolinic acid. Based on these results, we propose that DNA gyrase participates in a concerted reaction with DNA topoisomerases in the immediate relaxation of DNA in cells exposed to heat shock.
AB - Genetic studies revealed that DNA gyrase seems to catalyze immediate and transient DNA relaxation after Escherichia coli cells are exposed to heat shock (Ogata, Y., Mizushima, T., Kataoka, K., Miki, T., and Sekimizu, K. (1994) Mol. Gen. Genet. 244, 451-455). We have now obtained biochemical evidence to support this hypothesis. DNA gyrase catalyzed an increase in the linking number of DNA and relaxation of negatively supercoiled DNA, under physiological concentrations of ATP. Analyses by gel filtration chromatography of each subunit revealed that DNA relaxation activity co- migrated with each subunit. The linking number of DNA increased as the temperature increased. Further, the reaction was inhibited by nalidixic acid or by oxolinic acid. Based on these results, we propose that DNA gyrase participates in a concerted reaction with DNA topoisomerases in the immediate relaxation of DNA in cells exposed to heat shock.
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U2 - 10.1074/jbc.271.40.24806
DO - 10.1074/jbc.271.40.24806
M3 - Article
C2 - 8798753
AN - SCOPUS:0029780372
SN - 0021-9258
VL - 271
SP - 24806
EP - 24810
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 40
ER -