Heat shock induces differentiation of human embryonal carcinoma cells into trophectoderm lineages

Tatsuya Maruyama, Akihiro Umezawa, Satoshi Kusakari, Haruhito Kikuchi, Masami Nozaki, Jun Ichi Hata

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NCR-G3 cells were established from a testicular embryonal carcinoma and are highly multipotential, differentiating into trophectoderm cells upon exposure to retinoic acid. Differentiated NCR-G3 cells begin to produce human chorionic gonadotropin (hCG), a trophectoderm-specific hormone. We have previously isolated the up-regulated genes at the early stage of differentiation. One of them was found to be a heat shock protein gene. The heat shock protein gene (HSP90) is induced at the early stage of differentiation and decreases to the basal level or under the basal level at the later stage. We speculate that heat shock per se induces the differentiation of human EC cells. With exposure to heat, NCR-G3 cells began to express a series of differentiation markers such as cytokeratin and hCG. Heat, which is classically known to induce heat shock proteins, is able to differentiate an embryonal cell line into trophectoderm lineages, implying a new recognized function of a heat-like event in early differentiation.

Original languageEnglish
Pages (from-to)123-127
Number of pages5
JournalExperimental Cell Research
Issue number1
Publication statusPublished - 1996 Apr 10


ASJC Scopus subject areas

  • Cell Biology

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