Heating drug delivery to vascular wall with Rhodamine B and fluorescence labeled Paclitaxel ranging 50 to 70°C: Ex vivo study

R. Homma, M. Shinozuka, N. Shimazaki, Tsunenori Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

We studied heating drug delivery to vascular wall with Rhodamine B ranging 50 to 70°C ex vivo study. Porcine carotid artery was dipped in the heated Rhodamine B solution in 15 s and then cooled by 37°C saline. Rhodamine B concentration distribution in the vascular wall cross-section was measured by a fluorescence microscope using 550 nm for excitation and 620 nm emission for fluorescence detection. The total amount of measured fluorescence in the vascular wall was calculated as a indication of delivered Rhodamine B quantity. The delivered Rhodamine B quantity was increased with increasing heating temperature with 50 to 70°C. In the cases of 60 to 70°C heating, the delivered Rhodamine B quantity was 3.1 to 23.3 fold by that of 37°C. Defined penetration depth of the delivered Rhodamine B in the vascular wall was also significantly increased with 65°C and 70°C heating. We also studied heating drug delivery to the vascular wall with fluorescence labeled Paclitaxel with 70°C in 15 s and 60 s heating ex vivo. In both contact duration, the delivered Paclitaxel quantity was increased. To understand these drug delivery enhancement effects, we investigated the vascular cross-sectional structure change by the heating. Some holes over 50 nm in diameter appeared on the internal elastic lamina with 70°C heating. We prospected that vascular surface structure change by the heating might enhance drug delivery to the vascular wall.

Original languageEnglish
Title of host publicationOptical Interactions with Tissue and Cells XXVII
PublisherSPIE
Volume9706
ISBN (Electronic)9781628419405
DOIs
Publication statusPublished - 2016
EventOptical Interactions with Tissue and Cells XXVII - San Francisco, United States
Duration: 2016 Feb 142016 Feb 17

Other

OtherOptical Interactions with Tissue and Cells XXVII
CountryUnited States
CitySan Francisco
Period16/2/1416/2/17

Keywords

  • drug delivery
  • heating
  • Paclitaxel
  • vascular wall

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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    Homma, R., Shinozuka, M., Shimazaki, N., & Arai, T. (2016). Heating drug delivery to vascular wall with Rhodamine B and fluorescence labeled Paclitaxel ranging 50 to 70°C: Ex vivo study. In Optical Interactions with Tissue and Cells XXVII (Vol. 9706). [97060G] SPIE. https://doi.org/10.1117/12.2210780