Helicobacter pylori-associated gastric pro- and antioxidant formation in mongolian gerbils

Hidekazu Suzuki, Mikiji Mori, Koichi Seto, Akemi Kai, Chizuko Kawaguchi, Masayuki Suzuki, Makoto Suematsu, Tomoyuki Yoneta, Soichiro Miura, Hiromasa Ishii

Research output: Contribution to journalArticle

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Abstract

Helicobacter pylori colonized gastric mucosa is manifest in a significant neutrophil infiltration with an extensive level of oxyradical formation. Mongolian gerbil is one of the excellent models for H. pylori- infection. The present study was designed to investigate pro- and antioxidant formation in the stomach of H. pylori-positive gerbils. Fourteen male Mongolian gerbils (MGS/Sea) were orally inoculated with H. pylori (ATCC43504) (Hp group) and 15 gerbils were inoculated with the culture media (Control). H. pylori infection was confirmed by the serum anti-H. pylori IgG test. Each gerbil was evaluated 6 or 12 weeks after the inoculation. Neutrophil infiltration was assessed by the tissue MPO activity. Mucosal oxidative stress was evaluated by thiobarbituric acid-reactive substances (TBARS), total glutathione contents, glutathione peroxidase (GSHPx) activity and Cu-, Zn-superoxide dismutase (SOD) activity. In Hp group, the H. pylori was persistently infected until 12 weeks. The level of MPO activity was significantly higher in Hp group at 6 and 12 weeks. Although the levels of TBARS and total glutathione were within the same range as controls at 6 weeks, they were significantly increased at 12 weeks. However, GSHPx activity was significantly increased at 6 weeks, but became the same range with the controls at 12 weeks. SOD activity showed no significant increase in Hp group at 6 and 12 weeks. In conclusion, H. pylori inoculation induced gastric mucosal neutrophil activation and pro-oxidant formation and also increased total glutathione contents, one of the mucosal antioxidants in gerbils.

Original languageEnglish
Pages (from-to)679-684
Number of pages6
JournalFree Radical Biology and Medicine
Volume26
Issue number5-6
DOIs
Publication statusPublished - 1999 Mar

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Gerbillinae
Helicobacter pylori
Glutathione
Stomach
Thiobarbituric Acid Reactive Substances
Antioxidants
Infiltration
Oxidative stress
Glutathione Peroxidase
Superoxide Dismutase
Culture Media
Neutrophil Infiltration
Reactive Oxygen Species
Helicobacter Infections
Immunoglobulin G
Chemical activation
Tissue
Neutrophil Activation
Gastric Mucosa
Oceans and Seas

Keywords

  • Free radical
  • Glutathione
  • Leukocyte infiltration
  • Lipid peroxidation
  • Superoxide dismutase

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Suzuki, H., Mori, M., Seto, K., Kai, A., Kawaguchi, C., Suzuki, M., ... Ishii, H. (1999). Helicobacter pylori-associated gastric pro- and antioxidant formation in mongolian gerbils. Free Radical Biology and Medicine, 26(5-6), 679-684. https://doi.org/10.1016/S0891-5849(98)00248-2

Helicobacter pylori-associated gastric pro- and antioxidant formation in mongolian gerbils. / Suzuki, Hidekazu; Mori, Mikiji; Seto, Koichi; Kai, Akemi; Kawaguchi, Chizuko; Suzuki, Masayuki; Suematsu, Makoto; Yoneta, Tomoyuki; Miura, Soichiro; Ishii, Hiromasa.

In: Free Radical Biology and Medicine, Vol. 26, No. 5-6, 03.1999, p. 679-684.

Research output: Contribution to journalArticle

Suzuki, H, Mori, M, Seto, K, Kai, A, Kawaguchi, C, Suzuki, M, Suematsu, M, Yoneta, T, Miura, S & Ishii, H 1999, 'Helicobacter pylori-associated gastric pro- and antioxidant formation in mongolian gerbils', Free Radical Biology and Medicine, vol. 26, no. 5-6, pp. 679-684. https://doi.org/10.1016/S0891-5849(98)00248-2
Suzuki, Hidekazu ; Mori, Mikiji ; Seto, Koichi ; Kai, Akemi ; Kawaguchi, Chizuko ; Suzuki, Masayuki ; Suematsu, Makoto ; Yoneta, Tomoyuki ; Miura, Soichiro ; Ishii, Hiromasa. / Helicobacter pylori-associated gastric pro- and antioxidant formation in mongolian gerbils. In: Free Radical Biology and Medicine. 1999 ; Vol. 26, No. 5-6. pp. 679-684.
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