Helicobacter pylori-associated gastric pro- and antioxidant formation in mongolian gerbils

Hidekazu Suzuki, Mikiji Mori, Koichi Seto, Akemi Kai, Chizuko Kawaguchi, Masayuki Suzuki, Makoto Suematsu, Tomoyuki Yoneta, Soichiro Miura, Hiromasa Ishii

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54 Citations (Scopus)

Abstract

Helicobacter pylori colonized gastric mucosa is manifest in a significant neutrophil infiltration with an extensive level of oxyradical formation. Mongolian gerbil is one of the excellent models for H. pylori- infection. The present study was designed to investigate pro- and antioxidant formation in the stomach of H. pylori-positive gerbils. Fourteen male Mongolian gerbils (MGS/Sea) were orally inoculated with H. pylori (ATCC43504) (Hp group) and 15 gerbils were inoculated with the culture media (Control). H. pylori infection was confirmed by the serum anti-H. pylori IgG test. Each gerbil was evaluated 6 or 12 weeks after the inoculation. Neutrophil infiltration was assessed by the tissue MPO activity. Mucosal oxidative stress was evaluated by thiobarbituric acid-reactive substances (TBARS), total glutathione contents, glutathione peroxidase (GSHPx) activity and Cu-, Zn-superoxide dismutase (SOD) activity. In Hp group, the H. pylori was persistently infected until 12 weeks. The level of MPO activity was significantly higher in Hp group at 6 and 12 weeks. Although the levels of TBARS and total glutathione were within the same range as controls at 6 weeks, they were significantly increased at 12 weeks. However, GSHPx activity was significantly increased at 6 weeks, but became the same range with the controls at 12 weeks. SOD activity showed no significant increase in Hp group at 6 and 12 weeks. In conclusion, H. pylori inoculation induced gastric mucosal neutrophil activation and pro-oxidant formation and also increased total glutathione contents, one of the mucosal antioxidants in gerbils.

Original languageEnglish
Pages (from-to)679-684
Number of pages6
JournalFree Radical Biology and Medicine
Volume26
Issue number5-6
DOIs
Publication statusPublished - 1999 Mar 1

Keywords

  • Free radical
  • Glutathione
  • Leukocyte infiltration
  • Lipid peroxidation
  • Superoxide dismutase

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

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    Suzuki, H., Mori, M., Seto, K., Kai, A., Kawaguchi, C., Suzuki, M., Suematsu, M., Yoneta, T., Miura, S., & Ishii, H. (1999). Helicobacter pylori-associated gastric pro- and antioxidant formation in mongolian gerbils. Free Radical Biology and Medicine, 26(5-6), 679-684. https://doi.org/10.1016/S0891-5849(98)00248-2