Helicobacter pylori elicits gastric mucosal cell damage associated with neutrophil-derived toxic oxidants

M. Suzuki, S. Miura, M. Suematsu, D. Fukumura, H. Suzuki, A. Kai, M. Tsuchiya

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Purpose: To investigate Helicobacter pylori-dependent neutrophil activation and the in vitro effects of neutrophil-derived toxic oxidants on gastric mucosal cell injury. Methods and results: The luminol-dependent chemiluminescence activity of human neutrophils increased significantly after H. pylori was added, indicating that the oxidative burst of neutrophils was actually conducted by H pylori. The cytotoxicity of cultured gastric mucosal cells was assessed by the release of 2,7-bis-carboxyethyl-5(6)-carboxyfluorescein fluorescence from cellular cytoplasm. Neither H. pylori nor neutrophils alone produced significant epithelial cell injury but considerable damage was inflicted by H. pylori-activated neutrophils. Moreover, the epithelial cell injury was aggravated by ammonia (1 mmol/l) and inhibited by scavengers of reactive oxygen metabolites (catalase, taurine). Conclusions: These observations suggest that gastric mucosal damage may be elicited by H. pylori-activated neutrophils, which is, in part, regulated by reactive oxygen metabolites. It also appears that the ammonia-dependent oxidant, monochloramine, plays a unique part in this mucosal injury.

Original languageEnglish
Pages (from-to)S35-S39
JournalEuropean Journal of Gastroenterology and Hepatology
Volume5
Issue numberSUPPL. 1
Publication statusPublished - 1993 Jan 1

Keywords

  • Gastric mucosal cell
  • Helicobacter pylori
  • Neutrophils
  • Reactive oxygen

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

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