Helicobacter Pylori infection induces candidate stem cell marker musashi-1 in the human gastric epithelium

Hiroaki Murata, Shingo Tsuji, Masahiko Tsujii, Toru Nakamura, Hai Ying Fu, Hiroshi Eguchi, Kayoko Asahi, Hideyuki Okano, Sunao Kawano, Norio Hayashi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Musashi-1 (Msi-1), a mammalian neural RNA-binding protein, has been found to play important roles in the maintenance of stem cell states and differentiation in neural stem cells and mouse intestinal cells. We explored Msi-1 expression and its potential implications in the human stomach. Reverse transcription-PCR revealed that Msi-1 levels were significantly higher in the corpus than in antrum in Helicobacter pylori (Hp)-infected patients (n = 49) (P < 0.00001) in paired biopsy samples, whereas they were low and comparable at these two sites in Hp-negative patients (n = 31). Msi-1 levels were significantly higher in the Hp-infected corpus (n = 107) than in the Hp-negative corpus (n = 69) (P < 0.00000001). Immunohistochemistry and in situ hybridization demonstrated that Msi-1 was expressed at the base and neck/isthmus region of the fundic glands and partly co-expressed in Ki-67-positive cells in the corpus and antrum. Msi-1 levels correlated with Hp density (P < 0.05). Based on these results, we conclude that Hp infection strongly induces Msi-1 in the corpus. Given its expression in dividing cells, Msi-1 may modulate the state of gastric progenitor cells.

Original languageEnglish
Pages (from-to)363-369
Number of pages7
JournalDigestive Diseases and Sciences
Volume53
Issue number2
DOIs
Publication statusPublished - 2008 Feb

Fingerprint

Helicobacter Infections
Helicobacter pylori
Stomach
Stem Cells
Epithelium
RNA-Binding Proteins
Neural Stem Cells
Reverse Transcription
In Situ Hybridization
Cell Differentiation
Neck
Immunohistochemistry
Maintenance
Biopsy
Polymerase Chain Reaction

Keywords

  • Helicobacter pylori
  • Human
  • Musashi-1
  • Stem cell
  • Stomach

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Helicobacter Pylori infection induces candidate stem cell marker musashi-1 in the human gastric epithelium. / Murata, Hiroaki; Tsuji, Shingo; Tsujii, Masahiko; Nakamura, Toru; Fu, Hai Ying; Eguchi, Hiroshi; Asahi, Kayoko; Okano, Hideyuki; Kawano, Sunao; Hayashi, Norio.

In: Digestive Diseases and Sciences, Vol. 53, No. 2, 02.2008, p. 363-369.

Research output: Contribution to journalArticle

Murata, H, Tsuji, S, Tsujii, M, Nakamura, T, Fu, HY, Eguchi, H, Asahi, K, Okano, H, Kawano, S & Hayashi, N 2008, 'Helicobacter Pylori infection induces candidate stem cell marker musashi-1 in the human gastric epithelium', Digestive Diseases and Sciences, vol. 53, no. 2, pp. 363-369. https://doi.org/10.1007/s10620-007-9858-5
Murata, Hiroaki ; Tsuji, Shingo ; Tsujii, Masahiko ; Nakamura, Toru ; Fu, Hai Ying ; Eguchi, Hiroshi ; Asahi, Kayoko ; Okano, Hideyuki ; Kawano, Sunao ; Hayashi, Norio. / Helicobacter Pylori infection induces candidate stem cell marker musashi-1 in the human gastric epithelium. In: Digestive Diseases and Sciences. 2008 ; Vol. 53, No. 2. pp. 363-369.
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