Hematopoiesis-dependent expression of CD44 in murine hepatic progenitor cells

Shinya Ohata; Makiko Nawa; Takeshi Kasama; Tokiwa Yamasaki; Kenji Sawanobori; Shoji Hata; Takashi Nakamura; Yoichi Asaoka; Toshio Watanabe; Hitoshi Okamoto; Takahiko Hara; Shuji Terai; Isao Sakaida; Toshiaki Katada; Hiroshi Nishina

doi:10.1016/j.bbrc.2008.12.149

Hematopoiesis-dependent expression of CD44 in murine hepatic progenitor cells

Shinya Ohata, Makiko Nawa, Takeshi Kasama, Tokiwa Yamasaki, Kenji Sawanobori, Shoji Hata, Takashi Nakamura, Yoichi Asaoka, Toshio Watanabe, Hitoshi Okamoto, Takahiko Hara, Shuji Terai, Isao Sakaida, Toshiaki Katada, Hiroshi Nishina

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

The fetal liver serves as the predominant hematopoietic organ until birth. However, the mechanisms underlying this link between hematopoiesis and hepatogenesis are unclear. Previously, we reported the isolation of a monoclonal antibody (anti-Liv8) that specifically recognizes an antigen (Liv8) present in murine fetal livers at embryonic day 11.5 (E11.5). Liv8 is a cell surface molecule expressed by hematopoietic cells in both fetal liver and adult mouse bone marrow. Here, we report that Liv8 is also transiently expressed by hepatoblasts at E11.5. Using protein purification and mass spectrometry, we have identified Liv8 as the CD44 protein. Interestingly, the expression of Liv8/CD44 in fetal liver was completely lost in AML1^-/- murine embryos, which lack definitive hematopoiesis. These results show that hepatoblasts change from Liv8/CD44-negative to Liv8/CD44-positive status in a hematopoiesis-dependent manner by E11.5, and indicate that Liv8/CD44 expression is an important link between hematopoiesis and hepatogenesis during fetal liver development.

Original language	English
Pages (from-to)	817-823
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	379
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2008.12.149
Publication status	Published - 2009 Feb 20
Externally published	Yes

Keywords

AGM
AML1
CD44
Erythrocyte
Hematopoiesis
Hepatoblast
Hepatogenesis
Liv8
Liver
Liver development
Monoclonal antibody

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2008.12.149

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Ohata, S., Nawa, M., Kasama, T., Yamasaki, T., Sawanobori, K., Hata, S., Nakamura, T., Asaoka, Y., Watanabe, T., Okamoto, H., Hara, T., Terai, S., Sakaida, I., Katada, T., & Nishina, H. (2009). Hematopoiesis-dependent expression of CD44 in murine hepatic progenitor cells. Biochemical and Biophysical Research Communications, 379(4), 817-823. https://doi.org/10.1016/j.bbrc.2008.12.149

Ohata, S, Nawa, M, Kasama, T, Yamasaki, T, Sawanobori, K, Hata, S, Nakamura, T, Asaoka, Y, Watanabe, T, Okamoto, H, Hara, T, Terai, S, Sakaida, I, Katada, T & Nishina, H 2009, 'Hematopoiesis-dependent expression of CD44 in murine hepatic progenitor cells', Biochemical and Biophysical Research Communications, vol. 379, no. 4, pp. 817-823. https://doi.org/10.1016/j.bbrc.2008.12.149

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title = "Hematopoiesis-dependent expression of CD44 in murine hepatic progenitor cells",

abstract = "The fetal liver serves as the predominant hematopoietic organ until birth. However, the mechanisms underlying this link between hematopoiesis and hepatogenesis are unclear. Previously, we reported the isolation of a monoclonal antibody (anti-Liv8) that specifically recognizes an antigen (Liv8) present in murine fetal livers at embryonic day 11.5 (E11.5). Liv8 is a cell surface molecule expressed by hematopoietic cells in both fetal liver and adult mouse bone marrow. Here, we report that Liv8 is also transiently expressed by hepatoblasts at E11.5. Using protein purification and mass spectrometry, we have identified Liv8 as the CD44 protein. Interestingly, the expression of Liv8/CD44 in fetal liver was completely lost in AML1-/- murine embryos, which lack definitive hematopoiesis. These results show that hepatoblasts change from Liv8/CD44-negative to Liv8/CD44-positive status in a hematopoiesis-dependent manner by E11.5, and indicate that Liv8/CD44 expression is an important link between hematopoiesis and hepatogenesis during fetal liver development.",

keywords = "AGM, AML1, CD44, Erythrocyte, Hematopoiesis, Hepatoblast, Hepatogenesis, Liv8, Liver, Liver development, Monoclonal antibody",

author = "Shinya Ohata and Makiko Nawa and Takeshi Kasama and Tokiwa Yamasaki and Kenji Sawanobori and Shoji Hata and Takashi Nakamura and Yoichi Asaoka and Toshio Watanabe and Hitoshi Okamoto and Takahiko Hara and Shuji Terai and Isao Sakaida and Toshiaki Katada and Hiroshi Nishina",

note = "Funding Information: This work was supported in part by research grants (to H.N.) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan, and the Japan Society for the Promotion of Science (JSPS). We are grateful to Drs. Minoru Tanaka, Atsushi Miyajima, Yuki Nakayama, Takahiro Ito, and numerous members of the Nishina and Katada laboratories for critical reading and helpful discussions. ",

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T1 - Hematopoiesis-dependent expression of CD44 in murine hepatic progenitor cells

AU - Ohata, Shinya

AU - Nawa, Makiko

AU - Kasama, Takeshi

AU - Yamasaki, Tokiwa

AU - Sawanobori, Kenji

AU - Hata, Shoji

AU - Nakamura, Takashi

AU - Asaoka, Yoichi

AU - Watanabe, Toshio

AU - Okamoto, Hitoshi

AU - Hara, Takahiko

AU - Terai, Shuji

AU - Sakaida, Isao

AU - Katada, Toshiaki

AU - Nishina, Hiroshi

N1 - Funding Information: This work was supported in part by research grants (to H.N.) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan, and the Japan Society for the Promotion of Science (JSPS). We are grateful to Drs. Minoru Tanaka, Atsushi Miyajima, Yuki Nakayama, Takahiro Ito, and numerous members of the Nishina and Katada laboratories for critical reading and helpful discussions.

PY - 2009/2/20

Y1 - 2009/2/20

N2 - The fetal liver serves as the predominant hematopoietic organ until birth. However, the mechanisms underlying this link between hematopoiesis and hepatogenesis are unclear. Previously, we reported the isolation of a monoclonal antibody (anti-Liv8) that specifically recognizes an antigen (Liv8) present in murine fetal livers at embryonic day 11.5 (E11.5). Liv8 is a cell surface molecule expressed by hematopoietic cells in both fetal liver and adult mouse bone marrow. Here, we report that Liv8 is also transiently expressed by hepatoblasts at E11.5. Using protein purification and mass spectrometry, we have identified Liv8 as the CD44 protein. Interestingly, the expression of Liv8/CD44 in fetal liver was completely lost in AML1-/- murine embryos, which lack definitive hematopoiesis. These results show that hepatoblasts change from Liv8/CD44-negative to Liv8/CD44-positive status in a hematopoiesis-dependent manner by E11.5, and indicate that Liv8/CD44 expression is an important link between hematopoiesis and hepatogenesis during fetal liver development.

AB - The fetal liver serves as the predominant hematopoietic organ until birth. However, the mechanisms underlying this link between hematopoiesis and hepatogenesis are unclear. Previously, we reported the isolation of a monoclonal antibody (anti-Liv8) that specifically recognizes an antigen (Liv8) present in murine fetal livers at embryonic day 11.5 (E11.5). Liv8 is a cell surface molecule expressed by hematopoietic cells in both fetal liver and adult mouse bone marrow. Here, we report that Liv8 is also transiently expressed by hepatoblasts at E11.5. Using protein purification and mass spectrometry, we have identified Liv8 as the CD44 protein. Interestingly, the expression of Liv8/CD44 in fetal liver was completely lost in AML1-/- murine embryos, which lack definitive hematopoiesis. These results show that hepatoblasts change from Liv8/CD44-negative to Liv8/CD44-positive status in a hematopoiesis-dependent manner by E11.5, and indicate that Liv8/CD44 expression is an important link between hematopoiesis and hepatogenesis during fetal liver development.

KW - AGM

KW - AML1

KW - CD44

KW - Erythrocyte

KW - Hematopoiesis

KW - Hepatoblast

KW - Hepatogenesis

KW - Liv8

KW - Liver

KW - Liver development

KW - Monoclonal antibody

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M3 - Article

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SN - 0006-291X

VL - 379

SP - 817

EP - 823

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Hematopoiesis-dependent expression of CD44 in murine hepatic progenitor cells

Abstract

Keywords

ASJC Scopus subject areas

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