Hematopoiesis-dependent expression of CD44 in murine hepatic progenitor cells

Shinya Ohata, Makiko Nawa, Takeshi Kasama, Tokiwa Yamasaki, Kenji Sawanobori, Shoji Hata, Takashi Nakamura, Yoichi Asaoka, Toshio Watanabe, Hitoshi Okamoto, Takahiko Hara, Shuji Terai, Isao Sakaida, Toshiaki Katada, Hiroshi Nishina

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The fetal liver serves as the predominant hematopoietic organ until birth. However, the mechanisms underlying this link between hematopoiesis and hepatogenesis are unclear. Previously, we reported the isolation of a monoclonal antibody (anti-Liv8) that specifically recognizes an antigen (Liv8) present in murine fetal livers at embryonic day 11.5 (E11.5). Liv8 is a cell surface molecule expressed by hematopoietic cells in both fetal liver and adult mouse bone marrow. Here, we report that Liv8 is also transiently expressed by hepatoblasts at E11.5. Using protein purification and mass spectrometry, we have identified Liv8 as the CD44 protein. Interestingly, the expression of Liv8/CD44 in fetal liver was completely lost in AML1-/- murine embryos, which lack definitive hematopoiesis. These results show that hepatoblasts change from Liv8/CD44-negative to Liv8/CD44-positive status in a hematopoiesis-dependent manner by E11.5, and indicate that Liv8/CD44 expression is an important link between hematopoiesis and hepatogenesis during fetal liver development.

Original languageEnglish
Pages (from-to)817-823
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume379
Issue number4
DOIs
Publication statusPublished - 2009 Feb 20
Externally publishedYes

Fingerprint

Hematopoiesis
Liver
Hepatocytes
Stem Cells
Fetal Development
Purification
Mass spectrometry
Mass Spectrometry
Bone
Proteins
Embryonic Structures
Bone Marrow
Monoclonal Antibodies
Parturition
Antigens
Molecules

Keywords

  • AGM
  • AML1
  • CD44
  • Erythrocyte
  • Hematopoiesis
  • Hepatoblast
  • Hepatogenesis
  • Liv8
  • Liver
  • Liver development
  • Monoclonal antibody

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Hematopoiesis-dependent expression of CD44 in murine hepatic progenitor cells. / Ohata, Shinya; Nawa, Makiko; Kasama, Takeshi; Yamasaki, Tokiwa; Sawanobori, Kenji; Hata, Shoji; Nakamura, Takashi; Asaoka, Yoichi; Watanabe, Toshio; Okamoto, Hitoshi; Hara, Takahiko; Terai, Shuji; Sakaida, Isao; Katada, Toshiaki; Nishina, Hiroshi.

In: Biochemical and Biophysical Research Communications, Vol. 379, No. 4, 20.02.2009, p. 817-823.

Research output: Contribution to journalArticle

Ohata, S, Nawa, M, Kasama, T, Yamasaki, T, Sawanobori, K, Hata, S, Nakamura, T, Asaoka, Y, Watanabe, T, Okamoto, H, Hara, T, Terai, S, Sakaida, I, Katada, T & Nishina, H 2009, 'Hematopoiesis-dependent expression of CD44 in murine hepatic progenitor cells', Biochemical and Biophysical Research Communications, vol. 379, no. 4, pp. 817-823. https://doi.org/10.1016/j.bbrc.2008.12.149
Ohata, Shinya ; Nawa, Makiko ; Kasama, Takeshi ; Yamasaki, Tokiwa ; Sawanobori, Kenji ; Hata, Shoji ; Nakamura, Takashi ; Asaoka, Yoichi ; Watanabe, Toshio ; Okamoto, Hitoshi ; Hara, Takahiko ; Terai, Shuji ; Sakaida, Isao ; Katada, Toshiaki ; Nishina, Hiroshi. / Hematopoiesis-dependent expression of CD44 in murine hepatic progenitor cells. In: Biochemical and Biophysical Research Communications. 2009 ; Vol. 379, No. 4. pp. 817-823.
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