Hematopoietic cell-specific adapter proteins, SLP-76 and BLNK, effectively activate NF-AT as well as NF-κB by Syk and Tec PTKs in non-lymphoid cell lines

Tetsuya Yamamoto, Tadashi Matsuda, Akira Junicho, Hiroyuki Kishi, Akihiko Yoshimura, Atsushi Muraguchi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

To investigate the roles of various hematopoietic cell-specific adapter proteins in T cell receptor (TCR)-signaling leading to nuclear factor of activated T cell (NF-AT) and nuclear factor of κB (NF-κB) activation, we reconstituted TCR-signaling with CD8/ζ, various protein tyrosine kinases (PTKs), and adapter proteins in a non-lymphoid cell line, 293T. We show that SLP-76 and BLNK, but not LAT, effectively co-operated with Syk and Tec family PTKs to activate NF-AT and NF-κB. We also show that Tec family PTKs enhanced endogenous phospholipase C (PLC)-γ1 phosphorylation induced by CD8/ζ and Syk in 293T cells. These results imply that PLC-γ1 may play a critical role in a hematopoietic cell-specific adapter protein-mediated NF-AT and NF-κB activation in a non-lymphoid cell.

Original languageEnglish
Pages (from-to)272-278
Number of pages7
JournalFEBS Letters
Volume491
Issue number3
DOIs
Publication statusPublished - 2001 Mar 2
Externally publishedYes

Fingerprint

NFATC Transcription Factors
Protein-Tyrosine Kinases
TCF Transcription Factors
Cells
Type C Phospholipases
T-Cell Antigen Receptor
Cell Line
Chemical activation
Phosphorylation
Proteins
HEK293 Cells
Tec protein-tyrosine kinase

Keywords

  • Adapter
  • Nuclear factor of κB
  • Nuclear factor of activated T cell
  • Signal transduction
  • Syk
  • T cell receptor
  • Tec

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Hematopoietic cell-specific adapter proteins, SLP-76 and BLNK, effectively activate NF-AT as well as NF-κB by Syk and Tec PTKs in non-lymphoid cell lines. / Yamamoto, Tetsuya; Matsuda, Tadashi; Junicho, Akira; Kishi, Hiroyuki; Yoshimura, Akihiko; Muraguchi, Atsushi.

In: FEBS Letters, Vol. 491, No. 3, 02.03.2001, p. 272-278.

Research output: Contribution to journalArticle

Yamamoto, Tetsuya ; Matsuda, Tadashi ; Junicho, Akira ; Kishi, Hiroyuki ; Yoshimura, Akihiko ; Muraguchi, Atsushi. / Hematopoietic cell-specific adapter proteins, SLP-76 and BLNK, effectively activate NF-AT as well as NF-κB by Syk and Tec PTKs in non-lymphoid cell lines. In: FEBS Letters. 2001 ; Vol. 491, No. 3. pp. 272-278.
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