Heme oxygenase-1 inhibits cytokine production by activated mast cells

Yumiko Yasui, Mao Nakamura, Toshihiro Onda, Tomohiro Uehara, Saori Murata, Nobuaki Matsui, Nobuyuki Fukuishi, Reiko Akagi, Makoto Suematsu, Masaaki Akagi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Heme oxygenase-1 (HO-1) is thought to contribute to host defense reactions against various stresses. In addition, recent reports have suggested that HO-1 modulates immunocyte activation and functions. HO-1 suppresses mast cell degranulation, but whether HO-1 suppresses cytokine synthesis as well is not yet known. We examined whether rat HO-1 cDNA transfected rat basophilic leukemia (RBL)-2H3 cells have altered cytokine production in response to stimulation with anti-ovalbumin (OA) serum/OA compared to Mock transfected RBL-2H3 cells. HO-1 inhibited anti-OA serum/OA-induced IL-3 and TNF-α production. Inhibition of HO-1 activity by Zn (II) protoporphyrin IX, a specific HO-1 inhibitor, prevented the suppression of TNF-α production. The cytokine inhibition by HO-1 was associated with selective suppression of the DNA-binding activity of AP-1 transcription factors. The suppression of mast cell cytokine production by HO-1 may be an important aspect of the processes that lead to resolution of allergic inflammation.

Original languageEnglish
Pages (from-to)485-490
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume354
Issue number2
DOIs
Publication statusPublished - 2007 Mar 9

Fingerprint

Heme Oxygenase-1
Mast Cells
Cytokines
Ovalbumin
Rats
Leukemia
Cell Degranulation
Interleukin-3
Transcription Factor AP-1
Serum
Complementary DNA
Chemical activation
Inflammation

Keywords

  • AP-1
  • Heme oxygenase-1
  • IL-3
  • Mast cell
  • TNF-α

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Yasui, Y., Nakamura, M., Onda, T., Uehara, T., Murata, S., Matsui, N., ... Akagi, M. (2007). Heme oxygenase-1 inhibits cytokine production by activated mast cells. Biochemical and Biophysical Research Communications, 354(2), 485-490. https://doi.org/10.1016/j.bbrc.2006.12.228

Heme oxygenase-1 inhibits cytokine production by activated mast cells. / Yasui, Yumiko; Nakamura, Mao; Onda, Toshihiro; Uehara, Tomohiro; Murata, Saori; Matsui, Nobuaki; Fukuishi, Nobuyuki; Akagi, Reiko; Suematsu, Makoto; Akagi, Masaaki.

In: Biochemical and Biophysical Research Communications, Vol. 354, No. 2, 09.03.2007, p. 485-490.

Research output: Contribution to journalArticle

Yasui, Y, Nakamura, M, Onda, T, Uehara, T, Murata, S, Matsui, N, Fukuishi, N, Akagi, R, Suematsu, M & Akagi, M 2007, 'Heme oxygenase-1 inhibits cytokine production by activated mast cells', Biochemical and Biophysical Research Communications, vol. 354, no. 2, pp. 485-490. https://doi.org/10.1016/j.bbrc.2006.12.228
Yasui, Yumiko ; Nakamura, Mao ; Onda, Toshihiro ; Uehara, Tomohiro ; Murata, Saori ; Matsui, Nobuaki ; Fukuishi, Nobuyuki ; Akagi, Reiko ; Suematsu, Makoto ; Akagi, Masaaki. / Heme oxygenase-1 inhibits cytokine production by activated mast cells. In: Biochemical and Biophysical Research Communications. 2007 ; Vol. 354, No. 2. pp. 485-490.
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