Hemicholinium-3-resistant choline uptake system linked to acetylcholine synthesis in the rat hippocampus

Takeshi Suzuki, Yuko Kashima, Koichiro Kawashima

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Extracellular choline-dependency of acetylcholine (ACh) synthesis was examined in rat hippocampal slices. In the presence of hemicholinium-3 (HC-3, 5 μM), extracellular choline-dependent ACh synthesis appeared to consist of two different components. The first component (saturated at up to 50 μM choline) was dependent on the HC-3-resistant choline uptake system having a low capacity for choline in comparison with the high-affinity choline uptake system (HACU). The second component, observed in the presence of more than 50 μM choline, was considered due to competitive inhibition of HACU by HC-3. HACU-dependent ACh synthesis seemed to be saturated in the presence of up to 2 μM choline. These results indicate that both the HACU and HC-3-resistant choline uptake systems are linked to ACh synthesis in the hippocampus.

Original languageEnglish
Pages (from-to)211-214
Number of pages4
JournalNeuroscience Letters
Volume105
Issue number1-2
DOIs
Publication statusPublished - 1989 Oct 23

Keywords

  • Acetylcholine synthesis
  • Choline
  • Hemicholinium-3
  • High-affinity choline uptake system
  • Hippocampus
  • Radioimmunoassay

ASJC Scopus subject areas

  • Neuroscience(all)

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