Hemopoietic stem cells in nude mice transplanted with colony-stimulating factor-producing tumors

H. Mizoguchi, T. Suda, Y. Miura, K. Kubota, F. Takaku

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

When pieces of a human pulmonary carcinoma which was producing colony-stimulating factor (CSF) were transplanted into nude mice a marked granulocytosis of 10 6/mm 3 was observed in the nude mice 4 weeks after the transplantation. In order to elucidate the control mechanism of hemopoiesis, we studied the kinetics of the hemopoietic stem cells of these mice. The number of GM-CFC, CFU(E), Meg-CFC and CFU(S) in the whole body increased to 6 times, 8 times, 6 times and 36 times as much respectively as those of the control mice. The increase in the number of these progenitors occurred mainly in the spleen. The sera of these mice contained only CSF. The cycling fraction of GM-CFC and CFU(S) determined by thymidine suicide technique was increased. From these findings, it could be considered that CSF produced in these mice acted directly or indirectly on CFU(S) including their differentiation into committed stem cells.

Original languageEnglish
Pages (from-to)874-880
Number of pages7
JournalExperimental Hematology
Volume10
Issue number10
Publication statusPublished - 1982

Fingerprint

Colony-Stimulating Factors
Nude Mice
Stem Cells
Granulocyte-Macrophage Progenitor Cells
Neoplasms
Erythroid Precursor Cells
Thymidine
Suicide
Spleen
Transplantation
Carcinoma
Lung
Serum

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Mizoguchi, H., Suda, T., Miura, Y., Kubota, K., & Takaku, F. (1982). Hemopoietic stem cells in nude mice transplanted with colony-stimulating factor-producing tumors. Experimental Hematology, 10(10), 874-880.

Hemopoietic stem cells in nude mice transplanted with colony-stimulating factor-producing tumors. / Mizoguchi, H.; Suda, T.; Miura, Y.; Kubota, K.; Takaku, F.

In: Experimental Hematology, Vol. 10, No. 10, 1982, p. 874-880.

Research output: Contribution to journalArticle

Mizoguchi, H, Suda, T, Miura, Y, Kubota, K & Takaku, F 1982, 'Hemopoietic stem cells in nude mice transplanted with colony-stimulating factor-producing tumors', Experimental Hematology, vol. 10, no. 10, pp. 874-880.
Mizoguchi, H. ; Suda, T. ; Miura, Y. ; Kubota, K. ; Takaku, F. / Hemopoietic stem cells in nude mice transplanted with colony-stimulating factor-producing tumors. In: Experimental Hematology. 1982 ; Vol. 10, No. 10. pp. 874-880.
@article{05861f7a60864d52979c83e5429d3ca0,
title = "Hemopoietic stem cells in nude mice transplanted with colony-stimulating factor-producing tumors",
abstract = "When pieces of a human pulmonary carcinoma which was producing colony-stimulating factor (CSF) were transplanted into nude mice a marked granulocytosis of 10 6/mm 3 was observed in the nude mice 4 weeks after the transplantation. In order to elucidate the control mechanism of hemopoiesis, we studied the kinetics of the hemopoietic stem cells of these mice. The number of GM-CFC, CFU(E), Meg-CFC and CFU(S) in the whole body increased to 6 times, 8 times, 6 times and 36 times as much respectively as those of the control mice. The increase in the number of these progenitors occurred mainly in the spleen. The sera of these mice contained only CSF. The cycling fraction of GM-CFC and CFU(S) determined by thymidine suicide technique was increased. From these findings, it could be considered that CSF produced in these mice acted directly or indirectly on CFU(S) including their differentiation into committed stem cells.",
author = "H. Mizoguchi and T. Suda and Y. Miura and K. Kubota and F. Takaku",
year = "1982",
language = "English",
volume = "10",
pages = "874--880",
journal = "Experimental Hematology",
issn = "0301-472X",
publisher = "Elsevier Inc.",
number = "10",

}

TY - JOUR

T1 - Hemopoietic stem cells in nude mice transplanted with colony-stimulating factor-producing tumors

AU - Mizoguchi, H.

AU - Suda, T.

AU - Miura, Y.

AU - Kubota, K.

AU - Takaku, F.

PY - 1982

Y1 - 1982

N2 - When pieces of a human pulmonary carcinoma which was producing colony-stimulating factor (CSF) were transplanted into nude mice a marked granulocytosis of 10 6/mm 3 was observed in the nude mice 4 weeks after the transplantation. In order to elucidate the control mechanism of hemopoiesis, we studied the kinetics of the hemopoietic stem cells of these mice. The number of GM-CFC, CFU(E), Meg-CFC and CFU(S) in the whole body increased to 6 times, 8 times, 6 times and 36 times as much respectively as those of the control mice. The increase in the number of these progenitors occurred mainly in the spleen. The sera of these mice contained only CSF. The cycling fraction of GM-CFC and CFU(S) determined by thymidine suicide technique was increased. From these findings, it could be considered that CSF produced in these mice acted directly or indirectly on CFU(S) including their differentiation into committed stem cells.

AB - When pieces of a human pulmonary carcinoma which was producing colony-stimulating factor (CSF) were transplanted into nude mice a marked granulocytosis of 10 6/mm 3 was observed in the nude mice 4 weeks after the transplantation. In order to elucidate the control mechanism of hemopoiesis, we studied the kinetics of the hemopoietic stem cells of these mice. The number of GM-CFC, CFU(E), Meg-CFC and CFU(S) in the whole body increased to 6 times, 8 times, 6 times and 36 times as much respectively as those of the control mice. The increase in the number of these progenitors occurred mainly in the spleen. The sera of these mice contained only CSF. The cycling fraction of GM-CFC and CFU(S) determined by thymidine suicide technique was increased. From these findings, it could be considered that CSF produced in these mice acted directly or indirectly on CFU(S) including their differentiation into committed stem cells.

UR - http://www.scopus.com/inward/record.url?scp=0020447961&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020447961&partnerID=8YFLogxK

M3 - Article

C2 - 6983976

AN - SCOPUS:0020447961

VL - 10

SP - 874

EP - 880

JO - Experimental Hematology

JF - Experimental Hematology

SN - 0301-472X

IS - 10

ER -