Hemostatic effects of polymerized albumin particles bearing rGPIa/IIa in thrombocytopenic mice

Yuji Teramura, Yosuke Okamura, Shinji Takeoka, Hiromi Tsuchiyama, Hideki Narumi, Mie Kainoh, Makoto Handa, Yasuo Ikeda, Eishun Tsuchida

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The recombinant fragment of the platelet membrane glycoprotein Ia/IIa (rGPIa/IIa) was conjugated to the polymerized albumin particles (polyAlb) with the average diameter of 180nm. The intravenous administration of rGPIa/IIa-polyAlb to thrombocytopenic mice ([platelet]=2.1±0.3×105particles/μL) with three doses of ca. 2.4×1010, 7.2×1010, and 2.4×1011particles/kg, respectively, significantly reduced their bleeding time to 426±71, 378±101, and 337±46s, respectively, whereas that of the control groups (PBS) was 730±198s. The injection of rGPIa/IIa-polyAlb (2.4×1011particles/kg) was approximately equal to the effect of the injection of the mouse platelets at a dose of 2.0×1010 particles/kg. It was confirmed that rGPIa/IIa-polyAlb had a recognition ability against collagen and could contribute to the hemostasis in the thrombocytopenic mice as a platelet substitute.

Original languageEnglish
Pages (from-to)256-260
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume306
Issue number1
DOIs
Publication statusPublished - 2003 Jun 20

Fingerprint

Bearings (structural)
Hemostatics
Albumins
Platelets
Blood Platelets
Integrin alpha2
Integrin alpha2beta1
Platelet Membrane Glycoproteins
Injections
Bleeding Time
Hemostasis
Intravenous Administration
Collagen
Control Groups

Keywords

  • Bleeding time
  • Collagen
  • GPIa/IIa
  • Platelet substitutes
  • Platelets
  • Polymerized albumin particles
  • Thrombocytopenic mice

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Hemostatic effects of polymerized albumin particles bearing rGPIa/IIa in thrombocytopenic mice. / Teramura, Yuji; Okamura, Yosuke; Takeoka, Shinji; Tsuchiyama, Hiromi; Narumi, Hideki; Kainoh, Mie; Handa, Makoto; Ikeda, Yasuo; Tsuchida, Eishun.

In: Biochemical and Biophysical Research Communications, Vol. 306, No. 1, 20.06.2003, p. 256-260.

Research output: Contribution to journalArticle

Teramura, Y, Okamura, Y, Takeoka, S, Tsuchiyama, H, Narumi, H, Kainoh, M, Handa, M, Ikeda, Y & Tsuchida, E 2003, 'Hemostatic effects of polymerized albumin particles bearing rGPIa/IIa in thrombocytopenic mice', Biochemical and Biophysical Research Communications, vol. 306, no. 1, pp. 256-260. https://doi.org/10.1016/S0006-291X(03)00943-4
Teramura, Yuji ; Okamura, Yosuke ; Takeoka, Shinji ; Tsuchiyama, Hiromi ; Narumi, Hideki ; Kainoh, Mie ; Handa, Makoto ; Ikeda, Yasuo ; Tsuchida, Eishun. / Hemostatic effects of polymerized albumin particles bearing rGPIa/IIa in thrombocytopenic mice. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 306, No. 1. pp. 256-260.
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