Hemostatic effects of polymerized albumin particles bearing rGPIa/IIa in thrombocytopenic mice

Yuji Teramura, Yosuke Okamura, Shinji Takeoka, Hiromi Tsuchiyama, Hideki Narumi, Mie Kainoh, Makoto Handa, Yasuo Ikeda, Eishun Tsuchida

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19 Citations (Scopus)

Abstract

The recombinant fragment of the platelet membrane glycoprotein Ia/IIa (rGPIa/IIa) was conjugated to the polymerized albumin particles (polyAlb) with the average diameter of 180nm. The intravenous administration of rGPIa/IIa-polyAlb to thrombocytopenic mice ([platelet]=2.1±0.3×105particles/μL) with three doses of ca. 2.4×1010, 7.2×1010, and 2.4×1011particles/kg, respectively, significantly reduced their bleeding time to 426±71, 378±101, and 337±46s, respectively, whereas that of the control groups (PBS) was 730±198s. The injection of rGPIa/IIa-polyAlb (2.4×1011particles/kg) was approximately equal to the effect of the injection of the mouse platelets at a dose of 2.0×1010 particles/kg. It was confirmed that rGPIa/IIa-polyAlb had a recognition ability against collagen and could contribute to the hemostasis in the thrombocytopenic mice as a platelet substitute.

Original languageEnglish
Pages (from-to)256-260
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume306
Issue number1
DOIs
Publication statusPublished - 2003 Jun 20

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Keywords

  • Bleeding time
  • Collagen
  • GPIa/IIa
  • Platelet substitutes
  • Platelets
  • Polymerized albumin particles
  • Thrombocytopenic mice

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Teramura, Y., Okamura, Y., Takeoka, S., Tsuchiyama, H., Narumi, H., Kainoh, M., Handa, M., Ikeda, Y., & Tsuchida, E. (2003). Hemostatic effects of polymerized albumin particles bearing rGPIa/IIa in thrombocytopenic mice. Biochemical and Biophysical Research Communications, 306(1), 256-260. https://doi.org/10.1016/S0006-291X(03)00943-4