Hepatic tumors induced by carbon tetrachloride in transgenic mice carrying a human c-H-ras proto-oncogene without mutations

S. Tsunematsu, Hidetsugu Saito, T. Kagawa, T. Morizane, J. Hata, T. Nakamura, H. Ishi, M. Tsuchiya, T. Nomura, M. Katsuki

Research output: Contribution to journalArticle

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Abstract

Hepatic tumors were generated in mice by repeated administration of carbon tetrachloride (CCl4). Eight transgenic (Tg) mice carrying a human c-H-ras proto-oncogene (rasH2 line) and 9 non-Tg mice were killed at 20 weeks. Tg mice developed more tumors than did non-Tg littermates. Most tumors were neoplastic nodules, but I hepatocellular carcinoma (HCC) was found in aTg mouse at 20 weeks. Three Tg and 2 non-Tg mice were kept without further administration of CCl4. Two Tg mice died at 30 weeks of HCC with intra-abdominal bleeding, and 1 Tg mouse developed HCC with a mesenteric metastasis at 32 weeks. No HCC was found in 2 non-Tg mice at 32 weeks. Although mutations at codon 12, 13, and 61 of the H-ras gene are often found in murine hepatocarcinogenesis, neither the tumors, including one HCC, nor the normal cells revealed any such mutations. These results showed that the unmutated human c-H-ras gene facilitates malignant transformation of hepatocytes when continuous liver-cell death and regeneration is caused by repeated administration of CCl4.

Original languageEnglish
Pages (from-to)554-559
Number of pages6
JournalInternational Journal of Cancer
Volume59
Issue number4
DOIs
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

ras Genes
Carbon Tetrachloride
Transgenic Mice
Hepatocellular Carcinoma
Mutation
Liver
Neoplasms
Codon
Regeneration
Hepatocytes
Cell Death
Hemorrhage
Neoplasm Metastasis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Hepatic tumors induced by carbon tetrachloride in transgenic mice carrying a human c-H-ras proto-oncogene without mutations. / Tsunematsu, S.; Saito, Hidetsugu; Kagawa, T.; Morizane, T.; Hata, J.; Nakamura, T.; Ishi, H.; Tsuchiya, M.; Nomura, T.; Katsuki, M.

In: International Journal of Cancer, Vol. 59, No. 4, 1994, p. 554-559.

Research output: Contribution to journalArticle

Tsunematsu, S, Saito, H, Kagawa, T, Morizane, T, Hata, J, Nakamura, T, Ishi, H, Tsuchiya, M, Nomura, T & Katsuki, M 1994, 'Hepatic tumors induced by carbon tetrachloride in transgenic mice carrying a human c-H-ras proto-oncogene without mutations', International Journal of Cancer, vol. 59, no. 4, pp. 554-559. https://doi.org/10.1002/ijc.2910590420
Tsunematsu, S. ; Saito, Hidetsugu ; Kagawa, T. ; Morizane, T. ; Hata, J. ; Nakamura, T. ; Ishi, H. ; Tsuchiya, M. ; Nomura, T. ; Katsuki, M. / Hepatic tumors induced by carbon tetrachloride in transgenic mice carrying a human c-H-ras proto-oncogene without mutations. In: International Journal of Cancer. 1994 ; Vol. 59, No. 4. pp. 554-559.
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AU - Hata, J.

AU - Nakamura, T.

AU - Ishi, H.

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AU - Nomura, T.

AU - Katsuki, M.

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