Hepatitis B Virus DNA Integration in Hepatocellular Carcinomas and Their Adjacent Non-Neoplastic Liver Tissues

Jing juan Ding; Hidetsugu Saito; Toshio Morizane; Tatehiro Kagawa; Shingo Matsumoto; Naoto Iwabuchi; Masaharu Tsuchiya; Naoki Kumagai; Tetsu Watanabe; Kanji Tsuchimoto

doi:10.2302/kjm.38.443

Hepatitis B Virus DNA Integration in Hepatocellular Carcinomas and Their Adjacent Non-Neoplastic Liver Tissues

Jing juan Ding, Hidetsugu Saito, Toshio Morizane, Tatehiro Kagawa, Shingo Matsumoto, Naoto Iwabuchi, Masaharu Tsuchiya, Naoki Kumagai, Tetsu Watanabe, Kanji Tsuchimoto

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Hepatitis B virus (HBV) DNA integration was studied in 24 hepatocellular carcinoma (HCC) tissues obtained at operations or autopsies. In 11 cases whose sera were positive for hepatitis B virus surface antigen (HBsAg), HBV DNA integration was demonstrated by Southern blot analysis. Only one of 6 cases whose sera were positive for hepatitis B surface antibody (HBsAb) but negative for HBsAg revealed the integration and the other 5 cases revealed no HBV DNA integration. HBV DNA amplification was noted in 4 of these 6 cases in which HBV DNA integration was found when compared with the adjacent liver tissues. The integration pattern of HBV DNA was different in one case between primary HCC tissue and a metastasized lymph node. It is suggested that HBV DNA amplification is not directly related to the development of HCC and that there are polyclonal tumor cells which have different patterns of virus genome integrations.

Original language	English
Pages (from-to)	443-453
Number of pages	11
Journal	the keio journal of medicine
Volume	38
Issue number	4
DOIs	https://doi.org/10.2302/kjm.38.443
Publication status	Published - 1989

Keywords

DNA
hepatitis B virus
hepatocellular carcinomas
integration

ASJC Scopus subject areas

Medicine(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.2302/kjm.38.443

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title = "Hepatitis B Virus DNA Integration in Hepatocellular Carcinomas and Their Adjacent Non-Neoplastic Liver Tissues",

abstract = "Hepatitis B virus (HBV) DNA integration was studied in 24 hepatocellular carcinoma (HCC) tissues obtained at operations or autopsies. In 11 cases whose sera were positive for hepatitis B virus surface antigen (HBsAg), HBV DNA integration was demonstrated by Southern blot analysis. Only one of 6 cases whose sera were positive for hepatitis B surface antibody (HBsAb) but negative for HBsAg revealed the integration and the other 5 cases revealed no HBV DNA integration. HBV DNA amplification was noted in 4 of these 6 cases in which HBV DNA integration was found when compared with the adjacent liver tissues. The integration pattern of HBV DNA was different in one case between primary HCC tissue and a metastasized lymph node. It is suggested that HBV DNA amplification is not directly related to the development of HCC and that there are polyclonal tumor cells which have different patterns of virus genome integrations.",

keywords = "DNA, hepatitis B virus, hepatocellular carcinomas, integration",

author = "Ding, {Jing juan} and Hidetsugu Saito and Toshio Morizane and Tatehiro Kagawa and Shingo Matsumoto and Naoto Iwabuchi and Masaharu Tsuchiya and Naoki Kumagai and Tetsu Watanabe and Kanji Tsuchimoto",

year = "1989",

doi = "10.2302/kjm.38.443",

language = "English",

volume = "38",

pages = "443--453",

journal = "Keio Journal of Medicine",

issn = "0022-9717",

publisher = "Keio University School of Medicine",

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TY - JOUR

T1 - Hepatitis B Virus DNA Integration in Hepatocellular Carcinomas and Their Adjacent Non-Neoplastic Liver Tissues

AU - Ding, Jing juan

AU - Saito, Hidetsugu

AU - Morizane, Toshio

AU - Kagawa, Tatehiro

AU - Matsumoto, Shingo

AU - Iwabuchi, Naoto

AU - Tsuchiya, Masaharu

AU - Kumagai, Naoki

AU - Watanabe, Tetsu

AU - Tsuchimoto, Kanji

PY - 1989

Y1 - 1989

N2 - Hepatitis B virus (HBV) DNA integration was studied in 24 hepatocellular carcinoma (HCC) tissues obtained at operations or autopsies. In 11 cases whose sera were positive for hepatitis B virus surface antigen (HBsAg), HBV DNA integration was demonstrated by Southern blot analysis. Only one of 6 cases whose sera were positive for hepatitis B surface antibody (HBsAb) but negative for HBsAg revealed the integration and the other 5 cases revealed no HBV DNA integration. HBV DNA amplification was noted in 4 of these 6 cases in which HBV DNA integration was found when compared with the adjacent liver tissues. The integration pattern of HBV DNA was different in one case between primary HCC tissue and a metastasized lymph node. It is suggested that HBV DNA amplification is not directly related to the development of HCC and that there are polyclonal tumor cells which have different patterns of virus genome integrations.

AB - Hepatitis B virus (HBV) DNA integration was studied in 24 hepatocellular carcinoma (HCC) tissues obtained at operations or autopsies. In 11 cases whose sera were positive for hepatitis B virus surface antigen (HBsAg), HBV DNA integration was demonstrated by Southern blot analysis. Only one of 6 cases whose sera were positive for hepatitis B surface antibody (HBsAb) but negative for HBsAg revealed the integration and the other 5 cases revealed no HBV DNA integration. HBV DNA amplification was noted in 4 of these 6 cases in which HBV DNA integration was found when compared with the adjacent liver tissues. The integration pattern of HBV DNA was different in one case between primary HCC tissue and a metastasized lymph node. It is suggested that HBV DNA amplification is not directly related to the development of HCC and that there are polyclonal tumor cells which have different patterns of virus genome integrations.

KW - DNA

KW - hepatitis B virus

KW - hepatocellular carcinomas

KW - integration

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U2 - 10.2302/kjm.38.443

DO - 10.2302/kjm.38.443

M3 - Article

C2 - 2560999

AN - SCOPUS:0024781539

SN - 0022-9717

VL - 38

SP - 443

EP - 453

JO - Keio Journal of Medicine

JF - Keio Journal of Medicine

IS - 4

ER -

Hepatitis B Virus DNA Integration in Hepatocellular Carcinomas and Their Adjacent Non-Neoplastic Liver Tissues

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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