Hepatoprotective effect of Combretum quadrangulare and its constituents

Arjun Hari Banskota, Yasuhiro Tezuka, Ketut I. Adnyana, Quanbo Xiong, Koji Hase, Kim Qui Tran, Ken Tanaka, Ikuo Saiki, Shigetoshi Kadota

Research output: Contribution to journalArticlepeer-review

72 Citations (Scopus)

Abstract

The MeOH extract of leaves of Combretum quadrangulare showed significant hepatoprotective effect on D-galactosamine (D-GalN)/lipopolysaccharide (LPS)- induced experimental fiver injury in mice and on D-GalN/tumor necrosis factor-α (TNF-α)-induced cell death in primary cultured mouse hepatocytes. Phytochemical investigation led to the isolation of thirty cycloartane-type triterpenes together with betulinic acid, β-sitosterol, β-sitosterol glucoside, 4 flavones (34-37), and 3 flavone C-glucosides (38-40). These compounds showed various potencies of hepatoprotective effect on D-GalN/TNF- α-induced cell death in primary cultured mouse hepatocytes. Quadrangularol B (29), methyl quadrangularate I (33), kamatakenin (34), 5,7,4'-trihydroxy- 3,3'-dimethoxyflavone (35), 5,4'-dihydroxy-3,7,3'-trimethoxyflavone (36) and isokaempferide (37) showed strong inhibitory effect on TNF-α-induced cell death with IC50 values of 34.3, 33.7, 13.3, 22.4, 13.4 and 22.8 μM, respectively, whereas clinically-used silibinin had an IC50 value of 39.6 μM and glycyrrhizin showed very weak inhibitory effect. Methyl quadrangularates A (30) and N (32), norquadrangularic acid B (31) and vitexin (40) also showed potent inhibition on TNF-α-induced cell death with IC50 values of 45.7, 89.3, 67.6 and 40.1 μM, respectively. The flavonoids and some of the cycloartane-type triterpenes appeared to be the hepatoprotective principles of the leaves of C. quadrangulare.

Original languageEnglish
Pages (from-to)456-460
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume23
Issue number4
DOIs
Publication statusPublished - 2000 Apr
Externally publishedYes

Keywords

  • Combretum quadrangulare
  • Cycloartane-type triterpene
  • Flavone
  • Flavone C-glycoside
  • Hepatoprotective effect

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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