Herpetiform pemphigus showing reactivity with pemphigus vulgaris antigen (desmoglein 3)

Akiharu Kubo, Masayuki Amagai, T. Hashimoto, T. Doi, M. Higashiyama, K. Hashimoto, K. Yoshikawa

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Abstract

We report a patient with herpetiform pemphigus (HP) who showed reactivity only with pemphigus vulgaris (PV) antigen but not with pemphigus foliaceus (PF) antigen. Direct and indirect immunofluorescence revealed keratinocyte cell surface staining in the lower layers of the epidermis, where desmoglein 3 (Dsg 3) is expressed. Immunoblot analysis, using ethylenediamine tetra-acetic acid-separated human epidermal extracts, revealed that the patient's serum recognized only a 130-kDa polypeptide which co-migrated with Dsg 3. By antigen-specific immunoadsorption studies, using desmoglein 1 (Dsg 1) and Dsg 3 recombinant protein produced by baculovirus expression system, immunoreactivity of the patient's serum was completely adsorbed by Dsg 3 alone, but not by Dsg 1. These results indicate that this HP patient produced only anti-Dsg3 autoantibodies and no other autoantibodies against components of the keratinocyte cell surface. HP could be a variant of PV, in addition to PE with unique clinical and histological features.

Original languageEnglish
Pages (from-to)109-113
Number of pages5
JournalBritish Journal of Dermatology
Volume137
Issue number1
Publication statusPublished - 1997

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Desmoglein 3
Pemphigus
Desmoglein 1
Antigens
ethylenediamine
Keratinocytes
Autoantibodies
Direct Fluorescent Antibody Technique
Baculoviridae
Cellular Structures
Indirect Fluorescent Antibody Technique
Serum
Recombinant Proteins
Epidermis
Acetic Acid
Staining and Labeling
Peptides

ASJC Scopus subject areas

  • Dermatology

Cite this

Kubo, A., Amagai, M., Hashimoto, T., Doi, T., Higashiyama, M., Hashimoto, K., & Yoshikawa, K. (1997). Herpetiform pemphigus showing reactivity with pemphigus vulgaris antigen (desmoglein 3). British Journal of Dermatology, 137(1), 109-113.

Herpetiform pemphigus showing reactivity with pemphigus vulgaris antigen (desmoglein 3). / Kubo, Akiharu; Amagai, Masayuki; Hashimoto, T.; Doi, T.; Higashiyama, M.; Hashimoto, K.; Yoshikawa, K.

In: British Journal of Dermatology, Vol. 137, No. 1, 1997, p. 109-113.

Research output: Contribution to journalArticle

Kubo, A, Amagai, M, Hashimoto, T, Doi, T, Higashiyama, M, Hashimoto, K & Yoshikawa, K 1997, 'Herpetiform pemphigus showing reactivity with pemphigus vulgaris antigen (desmoglein 3)', British Journal of Dermatology, vol. 137, no. 1, pp. 109-113.
Kubo, Akiharu ; Amagai, Masayuki ; Hashimoto, T. ; Doi, T. ; Higashiyama, M. ; Hashimoto, K. ; Yoshikawa, K. / Herpetiform pemphigus showing reactivity with pemphigus vulgaris antigen (desmoglein 3). In: British Journal of Dermatology. 1997 ; Vol. 137, No. 1. pp. 109-113.
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