Hes1-deficient mice show precocious differentiation of Paneth cells in the small intestine

Katsumasa Suzuki, Hirokazu Fukui, Takahisa Kayahara, Mitsutaka Sawada, Hiroshi Seno, Hiroshi Hiai, Ryoichiro Kageyama, Hideyuki Okano, Tsutomu Chiba

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

We have previously shown that Hes1 is expressed both in putative epithelial stem cells just above Paneth cells and in the crypt base columnar cells between Paneth cells, while Hes1 is completely absent in Paneth cells. This study was undertaken to clarify the role of Hes1 in Paneth cell differentiation, using Hes1-knockout (KO) newborn (P0) mice. Electron microscopy revealed premature appearance of distinct cells containing cytoplasmic granules in the intervillous region in Hes1-KO P0 mice, whereas those cells were absent in wild-type (WT) P0 mice. In Hes1-KO P0 mice, the gene expressions of cryptdins, exclusively present in Paneth cells, were all enhanced compared with WT P0 mice. Immunohistochemistry demonstrated increased number of both lysozyme-positive and cryptdin-4-positive cells in the small intestinal epithelium of Hes1-KO P0 mice as compared to WT P0 mice. Thus, Hes1 appears to have an inhibitory role in Paneth cell differentiation in the small intestine.

Original languageEnglish
Pages (from-to)348-352
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume328
Issue number1
DOIs
Publication statusPublished - 2005 Mar 4

Keywords

  • Cryptdin
  • Hes1
  • Musashi-1
  • Paneth cell
  • Small intestine
  • Stem cell

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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