Heterogeneity of hepatic acetaldehyde adducts in guinea-pigs after chronic ethanol administration

An immunohistochemical analysis with monoclonal and polyclonal antibodies against acetaldehyde-modified protein epitopes

Hirokazu Yokoyama, H. Ishii, S. Nagata, S. Moriya, T. Ito, Shinzo Kato, M. Tsuchiya

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Abstract

The formation of acetaldehyde (AcH) adducts was immunohistochemically demonstrated in the livers of experimental animals after chronic ethanol consumption. Recently, we established a hybridoma producing monoclonal antibody against the adduct. Although the polyclonal antibody obtained from a rabbit immunized with adducts had affinities for AcH adducts produced with 20 μM, 1 mM and 10 mM of AcH, the monoclonal antibody could recognize only those produced with 1 and 10 mM of AcH, suggesting that there is a difference of antigenecity between the adducts formed with a high concentration of AcH and those modified with a low concentration of AcH. AcH adducts in the liver of guinea-pigs fed ethanol for 90 days were detected by immunohistochemical staining with the polyclonal and monoclonal antibodies. The staining of liver specimens with polyclonal antibody was observed around both the portal and perivenular areas, whereas the reactions to monoclonal antibody were localized only in the perivenular area. These data suggest that AcH adducts are able to be formed around both portal and perivenular areas in the liver and that the perivenular area might be exposed to a higher concentration of AcH than the portal area after ethanol intake.

Original languageEnglish
Pages (from-to)91-97
Number of pages7
JournalAlcohol and Alcoholism
Volume28
Issue numberSUPPL. 1 A
Publication statusPublished - 1993

Fingerprint

Acetaldehyde
Epitopes
Guinea Pigs
Ethanol
Monoclonal Antibodies
Antibodies
Liver
Proteins
Staining and Labeling
Hybridomas
Animals
Rabbits

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology
  • Medicine(all)

Cite this

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title = "Heterogeneity of hepatic acetaldehyde adducts in guinea-pigs after chronic ethanol administration: An immunohistochemical analysis with monoclonal and polyclonal antibodies against acetaldehyde-modified protein epitopes",
abstract = "The formation of acetaldehyde (AcH) adducts was immunohistochemically demonstrated in the livers of experimental animals after chronic ethanol consumption. Recently, we established a hybridoma producing monoclonal antibody against the adduct. Although the polyclonal antibody obtained from a rabbit immunized with adducts had affinities for AcH adducts produced with 20 μM, 1 mM and 10 mM of AcH, the monoclonal antibody could recognize only those produced with 1 and 10 mM of AcH, suggesting that there is a difference of antigenecity between the adducts formed with a high concentration of AcH and those modified with a low concentration of AcH. AcH adducts in the liver of guinea-pigs fed ethanol for 90 days were detected by immunohistochemical staining with the polyclonal and monoclonal antibodies. The staining of liver specimens with polyclonal antibody was observed around both the portal and perivenular areas, whereas the reactions to monoclonal antibody were localized only in the perivenular area. These data suggest that AcH adducts are able to be formed around both portal and perivenular areas in the liver and that the perivenular area might be exposed to a higher concentration of AcH than the portal area after ethanol intake.",
author = "Hirokazu Yokoyama and H. Ishii and S. Nagata and S. Moriya and T. Ito and Shinzo Kato and M. Tsuchiya",
year = "1993",
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TY - JOUR

T1 - Heterogeneity of hepatic acetaldehyde adducts in guinea-pigs after chronic ethanol administration

T2 - An immunohistochemical analysis with monoclonal and polyclonal antibodies against acetaldehyde-modified protein epitopes

AU - Yokoyama, Hirokazu

AU - Ishii, H.

AU - Nagata, S.

AU - Moriya, S.

AU - Ito, T.

AU - Kato, Shinzo

AU - Tsuchiya, M.

PY - 1993

Y1 - 1993

N2 - The formation of acetaldehyde (AcH) adducts was immunohistochemically demonstrated in the livers of experimental animals after chronic ethanol consumption. Recently, we established a hybridoma producing monoclonal antibody against the adduct. Although the polyclonal antibody obtained from a rabbit immunized with adducts had affinities for AcH adducts produced with 20 μM, 1 mM and 10 mM of AcH, the monoclonal antibody could recognize only those produced with 1 and 10 mM of AcH, suggesting that there is a difference of antigenecity between the adducts formed with a high concentration of AcH and those modified with a low concentration of AcH. AcH adducts in the liver of guinea-pigs fed ethanol for 90 days were detected by immunohistochemical staining with the polyclonal and monoclonal antibodies. The staining of liver specimens with polyclonal antibody was observed around both the portal and perivenular areas, whereas the reactions to monoclonal antibody were localized only in the perivenular area. These data suggest that AcH adducts are able to be formed around both portal and perivenular areas in the liver and that the perivenular area might be exposed to a higher concentration of AcH than the portal area after ethanol intake.

AB - The formation of acetaldehyde (AcH) adducts was immunohistochemically demonstrated in the livers of experimental animals after chronic ethanol consumption. Recently, we established a hybridoma producing monoclonal antibody against the adduct. Although the polyclonal antibody obtained from a rabbit immunized with adducts had affinities for AcH adducts produced with 20 μM, 1 mM and 10 mM of AcH, the monoclonal antibody could recognize only those produced with 1 and 10 mM of AcH, suggesting that there is a difference of antigenecity between the adducts formed with a high concentration of AcH and those modified with a low concentration of AcH. AcH adducts in the liver of guinea-pigs fed ethanol for 90 days were detected by immunohistochemical staining with the polyclonal and monoclonal antibodies. The staining of liver specimens with polyclonal antibody was observed around both the portal and perivenular areas, whereas the reactions to monoclonal antibody were localized only in the perivenular area. These data suggest that AcH adducts are able to be formed around both portal and perivenular areas in the liver and that the perivenular area might be exposed to a higher concentration of AcH than the portal area after ethanol intake.

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