Heterophilic binding of the adhesion molecules poliovirus receptor and immunoglobulin superfamily 4A in the interaction between mouse spermatogenic and sertoli cells

Tomohiko Wakayama, Yoshimichi Sai, Akihiko Ito, Yukio Kato, Miho Kurobo, Yoshinori Murakami, Emi Nakashima, Akira Tsuji, Yukihiko Kitamura, Shoichi Iseki

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The cell adhesion protein immunoglobulin superfamily 4A (IGSF4A) is expressed on the surfaces of spermatogenic cells in the mouse testis. During spermatogenesis, IGSF4A is considered to bind to the surface of Sertoli cells in a heterophilic manner. To identify this unknown partner of IGSF4A, we generated rat monoclonal antibodies against the membrane proteins of mouse Sertoli cells grown in primary culture. Using these monoclonal antibodies, we isolated a clone that immunostained Sertoli cells and reacted with the product of immunoprecipitation of the homogenate of mouse testis with anti-IGSF4A antibody. Subsequently, to identify the Sertoli cell membrane protein that is recognized by this monoclonal antibody, we performed expression cloning of a cDNA library from the mouse testis. As a result, we identified poliovirus receptor (PVR), which is another IGSF-type cell adhesion molecule, as the binding partner of IGSF4A. The antibodies raised against PVR and IGSF4A immunoprecipitated both antigens in the homogenate of mouse testis. Immunoreactivity for PVR was present in Sertoli cells but not in spermatogenic cells at all stages of spermatogenesis. Overexpression of PVR in TM4, a mouse Sertoli cell line, increased more than three-fold its capacity to adhere to Tera-2, which is a human cell line that expresses IGSF4A. These findings suggest that the heterophilic binding of PVR to IGSF4A is responsible, at least in part, for the interaction between Sertoli and spermatogenic cells during mouse spermatogenesis.

Original languageEnglish
Pages (from-to)1081-1090
Number of pages10
JournalBiology of Reproduction
Volume76
Issue number6
DOIs
Publication statusPublished - 2007 Jun

Fingerprint

Sertoli Cells
Immunoglobulins
Testis
Spermatogenesis
Monoclonal Antibodies
Membrane Proteins
Cell Line
poliovirus receptor
Antibodies
Cell Adhesion Molecules
Gene Library
Immunoprecipitation
Cell Adhesion
Organism Cloning
Clone Cells
Antigens

Keywords

  • CD155
  • Necl-2
  • Necl-5
  • SglGSF
  • Spermatogenesis
  • Testis
  • TSLC1

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Heterophilic binding of the adhesion molecules poliovirus receptor and immunoglobulin superfamily 4A in the interaction between mouse spermatogenic and sertoli cells. / Wakayama, Tomohiko; Sai, Yoshimichi; Ito, Akihiko; Kato, Yukio; Kurobo, Miho; Murakami, Yoshinori; Nakashima, Emi; Tsuji, Akira; Kitamura, Yukihiko; Iseki, Shoichi.

In: Biology of Reproduction, Vol. 76, No. 6, 06.2007, p. 1081-1090.

Research output: Contribution to journalArticle

Wakayama, Tomohiko ; Sai, Yoshimichi ; Ito, Akihiko ; Kato, Yukio ; Kurobo, Miho ; Murakami, Yoshinori ; Nakashima, Emi ; Tsuji, Akira ; Kitamura, Yukihiko ; Iseki, Shoichi. / Heterophilic binding of the adhesion molecules poliovirus receptor and immunoglobulin superfamily 4A in the interaction between mouse spermatogenic and sertoli cells. In: Biology of Reproduction. 2007 ; Vol. 76, No. 6. pp. 1081-1090.
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