Hierarchal order in the formation of chloroplast division machinery in the red alga Cyanidioschyzon merolae

Nobuko Sumiya, Shin ya Miyagishim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Chloroplasts have evolved from a cyanobacterial endosymbiont and multiply by dividing. Chloroplast division is performed by constriction of the ring-like protein complex (the PD machinery), which forms at the division site. The PD machinery is composed of cyanobacteriadescended components such as FtsZ and eukaryote-derived proteins such as the dynamin-related protein, DRP5B. In the red alga Cyanidioschyzon merolae, FtsZ ring formation on the stromal side precedes PDR1 and DRP5B ring formation on the cytosolic side. In this study, we impaired FtsZ ring formation in C. merolae by overexpressing FtsZ just before FtsZ ring formation. As a result, PDR1 and DRP5B failed to localize at the chloroplast division site, suggesting that FtsZ ring formation is required for the PDR1 and DRP5B rings. We further found, by expressing a dominant negative form of DRP5B, that DRP5B ring formation begins on the nuclear side of the chloroplast division site. These findings provide insight into how the PD machinery forms in red algae.

Original languageEnglish
Article numbere1294298
JournalCommunicative and Integrative Biology
Volume10
Issue number2
DOIs
Publication statusPublished - 2017 Mar 4
Externally publishedYes

Fingerprint

Rhodophyta
Chloroplasts
chloroplasts
Dynamins
Proteins
proteins
endosymbionts
Eukaryota
Constriction
eukaryotic cells

Keywords

  • Chloroplast division
  • Cyanidioschyzon merolae
  • DRP5B
  • FtsZ
  • PDR1
  • Plastid division machinery

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Hierarchal order in the formation of chloroplast division machinery in the red alga Cyanidioschyzon merolae. / Sumiya, Nobuko; Miyagishim, Shin ya.

In: Communicative and Integrative Biology, Vol. 10, No. 2, e1294298, 04.03.2017.

Research output: Contribution to journalArticle

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