Hierarchical Hydrogel Drug Delivery System Enables Controlled Release of Adeno-Associated Viruses for Gene Therapy

Hideo Miyahara, Yuta Kurashina, Yuki Ogawa, Ayumu Kurihara, Tomohiko Yoshida, Hirotaka James Okano, Masato Fujioka, Hiroaki Onoe

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper describes a drug delivery system (DDS) to release Adeno-associated viruses (AAVs) for gene therapy. Our system has two-level hierarchical structures made of different hydrogel materials: (i) Calcium alginate hydrogel microparticles (\sim 100\ \mu \mathrm{m} in diameter) that can properly immobilize AAVs inside and release them for gene transfection triggered by enzymatic degradation, and (ii) a millimeter-scale collagen sheet that encapsulates those alginate microparticles with AAVs for stable fixation to even a tiny and complicated diseased site in our body, such as an inner ear for the treatment of hereditary hearing loss (Figure 1). We fabricated the AAVs-immobilized hydrogel microparticles and evaluated their AAV releasing functions by measured the gene transfection ratio to cultured HeLa cells. In addition, we also demonstrated that the controlled release of AAVs immobilized in the alginate microparticles embedded in the millimeter-scale collagen sheet can be externally triggered from the outside. We envision that our developed composite hydrogel DDS could be an essential and general tool for gene therapy in practical medical scenes, especially in the treatment at complicated internal sites.

Original languageEnglish
Title of host publication2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages601-602
Number of pages2
ISBN (Electronic)9781728116105
DOIs
Publication statusPublished - 2019 Jan
Event32nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2019 - Seoul, Korea, Republic of
Duration: 2019 Jan 272019 Jan 31

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume2019-January
ISSN (Print)1084-6999

Conference

Conference32nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2019
CountryKorea, Republic of
CitySeoul
Period19/1/2719/1/31

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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  • Cite this

    Miyahara, H., Kurashina, Y., Ogawa, Y., Kurihara, A., Yoshida, T., Okano, H. J., Fujioka, M., & Onoe, H. (2019). Hierarchical Hydrogel Drug Delivery System Enables Controlled Release of Adeno-Associated Viruses for Gene Therapy. In 2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019 (pp. 601-602). [8870781] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2019-January). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2019.8870781