TY - JOUR
T1 - High- and low-LET induced chromosome damage in human lymphocytes
T2 - A time-course of aberrations in metaphase and interphase
AU - George, K.
AU - Wu, H.
AU - Willingham, V.
AU - Furusawa, Y.
AU - Kawata, T.
AU - Cucinotta, F. A.
N1 - Funding Information:
TheauthorsaregratefultothestaÚ ofthe Brookhaven National Laboratory for supporting the iron run and to Dr J. Miller and colleagues at Lawrence Berkeley Laboratory for performing dosimetry measurements. The work was supported by the NASA Space Radiation Health Program. T. K. is supported by a NRC grant (fellowship no. 9818170) and is on leave of the absence from Department of Radiology, School of medicine, Keio University, Tokyo, Japan. The authors are also grateful to Dr Marco Durante for his assistance in preparing this manuscript.
PY - 2001
Y1 - 2001
N2 - Purpose: To investigate how cell-cycle delays in human peripheral lymphocytes affect the expression of complex chromosome damage in metaphase following high- and low-LET radiation exposure. Materials and methods: Whole blood was irradiated in vitro with a low and a high dose of I GeV u-1 iron particles, 400 MeV u-1 neon particles or γ-rays. Lymphocytes were cultured and metaphase cells were collected at different time points after 48-84h in culture. Interphase chromosomes were prematurely condensed using calyculin-A, either 48 or 72h after exposure to iron particles or γ-rays. Cells in first division were analysed using a combination of FISH whole-chromosome painting and DAPI/Hoechst 33258 harlequin staining. Results: There was a delay in expression of chromosome damage in metaphase that was LET- and dose-dependant. This delay was mostly related to the late emergence of complex-type damage into metaphase. Yields of damage in PCC collected 48 h after irradiation with iron particles were similar to values obtained from cells undergoing mitosis after prolonged incubation. Conclusion: The yield of high-LET radiation-induced complex chromosome damage could be underestimated when analysing metaphase cells collected at one time point after irradiation. Chemically induced PCC is a more accurate technique since problems with complicated cell-cycle delays are avoided.
AB - Purpose: To investigate how cell-cycle delays in human peripheral lymphocytes affect the expression of complex chromosome damage in metaphase following high- and low-LET radiation exposure. Materials and methods: Whole blood was irradiated in vitro with a low and a high dose of I GeV u-1 iron particles, 400 MeV u-1 neon particles or γ-rays. Lymphocytes were cultured and metaphase cells were collected at different time points after 48-84h in culture. Interphase chromosomes were prematurely condensed using calyculin-A, either 48 or 72h after exposure to iron particles or γ-rays. Cells in first division were analysed using a combination of FISH whole-chromosome painting and DAPI/Hoechst 33258 harlequin staining. Results: There was a delay in expression of chromosome damage in metaphase that was LET- and dose-dependant. This delay was mostly related to the late emergence of complex-type damage into metaphase. Yields of damage in PCC collected 48 h after irradiation with iron particles were similar to values obtained from cells undergoing mitosis after prolonged incubation. Conclusion: The yield of high-LET radiation-induced complex chromosome damage could be underestimated when analysing metaphase cells collected at one time point after irradiation. Chemically induced PCC is a more accurate technique since problems with complicated cell-cycle delays are avoided.
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U2 - 10.1080/0955300001003760
DO - 10.1080/0955300001003760
M3 - Article
C2 - 11236924
AN - SCOPUS:0035136362
SN - 0955-3002
VL - 77
SP - 175
EP - 183
JO - International Journal of Radiation Biology
JF - International Journal of Radiation Biology
IS - 2
ER -