High CCL18/PARC expression in articular cartilage and synovial tissue of patients with rheumatoid arthritis

Shigeki Momohara, Hiroshi Okamoto, Takuji Iwamoto, Tamao Mizumura, Katsunori Ikari, Yasushi Kawaguchi, Masahiro Takeuchi, Naoyuki Kamatani, Taisuke Tomatsu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective. We studied the role of CCL18/pulmonary and activation-regulated chemokine (PARC) in rheumatoid arthritis (RA). Methods. Human cartilage tissues and synovial membranes were obtained from patients with RA and with osteoarthritis (OA). Sera samples were obtained from RA patients, OA patients, healthy controls, and patients with flu, and synovial fluid (SF) from patients with RA and OA. Real-time PCR was performed with RNA from cartilage samples. Immunohistochemical analysis of CCL18/PARC was done with RA and OA cartilage and synovial tissue. Levels of CCL18/PARC in serum and SF were evaluated by ELISA. Results. CCL18/PARC mRNA was expressed at significantly higher levels in RA cartilage than in OA (p = 0.0001) and control (p < 0.0001) samples. CCL18/PARC mRNA expression was much higher in RA synovial membrane than OA samples (p = 0.0001). All RA cartilage and synovial tissue samples exhibited medium to strong staining for CCL18/PARC. Serum levels of CCL18/PARC were higher in RA patients (156.21 ± 125.73 ng/ml, n = 71) than in OA patients (64.54 ± 40.90 ng/ml, n = 12) and controls (28.04 ± 10.96 ng/ml, n = 20). Levels of CCL18/PARC in RA SF (275.20 ± 228.16 ng/ml, n = 15) were higher than in OA (33.13 ± 14.84 ng/ml, n = 6; p = 0.0198). CCL18/PARC levels correlated significantly with rheumatoid factor levels (r = 0.431, p = 0.0040), but not with matrix metalloproteinase-3, erythrocyte sedimentation rate, and C-reactive protein. Conclusion. CCL18/PARC was highly expressed in RA articular cartilage and synovial tissue compared with OA samples. Our data indicated that CCL18/PARC levels are not related to the conditions of generalized inflammation, but are related to the pathogenesis of RA.

Original languageEnglish
Pages (from-to)266-271
Number of pages6
JournalJournal of Rheumatology
Volume34
Issue number2
Publication statusPublished - 2007 Feb
Externally publishedYes

Fingerprint

Articular Cartilage
Chemokines
Rheumatoid Arthritis
Osteoarthritis
Lung
Cartilage
Synovial Fluid
Synovial Membrane
Serum
Matrix Metalloproteinase 3
Messenger RNA
Rheumatoid Factor
Blood Sedimentation
C-Reactive Protein
Real-Time Polymerase Chain Reaction
Enzyme-Linked Immunosorbent Assay
RNA
Staining and Labeling
Inflammation

Keywords

  • Cartilage
  • CCL18/CC chemokine
  • Pulmonary and activation-regulated chemokine
  • Rheumatoid arthritis
  • Synovial tissue

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

Cite this

Momohara, S., Okamoto, H., Iwamoto, T., Mizumura, T., Ikari, K., Kawaguchi, Y., ... Tomatsu, T. (2007). High CCL18/PARC expression in articular cartilage and synovial tissue of patients with rheumatoid arthritis. Journal of Rheumatology, 34(2), 266-271.

High CCL18/PARC expression in articular cartilage and synovial tissue of patients with rheumatoid arthritis. / Momohara, Shigeki; Okamoto, Hiroshi; Iwamoto, Takuji; Mizumura, Tamao; Ikari, Katsunori; Kawaguchi, Yasushi; Takeuchi, Masahiro; Kamatani, Naoyuki; Tomatsu, Taisuke.

In: Journal of Rheumatology, Vol. 34, No. 2, 02.2007, p. 266-271.

Research output: Contribution to journalArticle

Momohara, S, Okamoto, H, Iwamoto, T, Mizumura, T, Ikari, K, Kawaguchi, Y, Takeuchi, M, Kamatani, N & Tomatsu, T 2007, 'High CCL18/PARC expression in articular cartilage and synovial tissue of patients with rheumatoid arthritis', Journal of Rheumatology, vol. 34, no. 2, pp. 266-271.
Momohara, Shigeki ; Okamoto, Hiroshi ; Iwamoto, Takuji ; Mizumura, Tamao ; Ikari, Katsunori ; Kawaguchi, Yasushi ; Takeuchi, Masahiro ; Kamatani, Naoyuki ; Tomatsu, Taisuke. / High CCL18/PARC expression in articular cartilage and synovial tissue of patients with rheumatoid arthritis. In: Journal of Rheumatology. 2007 ; Vol. 34, No. 2. pp. 266-271.
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abstract = "Objective. We studied the role of CCL18/pulmonary and activation-regulated chemokine (PARC) in rheumatoid arthritis (RA). Methods. Human cartilage tissues and synovial membranes were obtained from patients with RA and with osteoarthritis (OA). Sera samples were obtained from RA patients, OA patients, healthy controls, and patients with flu, and synovial fluid (SF) from patients with RA and OA. Real-time PCR was performed with RNA from cartilage samples. Immunohistochemical analysis of CCL18/PARC was done with RA and OA cartilage and synovial tissue. Levels of CCL18/PARC in serum and SF were evaluated by ELISA. Results. CCL18/PARC mRNA was expressed at significantly higher levels in RA cartilage than in OA (p = 0.0001) and control (p < 0.0001) samples. CCL18/PARC mRNA expression was much higher in RA synovial membrane than OA samples (p = 0.0001). All RA cartilage and synovial tissue samples exhibited medium to strong staining for CCL18/PARC. Serum levels of CCL18/PARC were higher in RA patients (156.21 ± 125.73 ng/ml, n = 71) than in OA patients (64.54 ± 40.90 ng/ml, n = 12) and controls (28.04 ± 10.96 ng/ml, n = 20). Levels of CCL18/PARC in RA SF (275.20 ± 228.16 ng/ml, n = 15) were higher than in OA (33.13 ± 14.84 ng/ml, n = 6; p = 0.0198). CCL18/PARC levels correlated significantly with rheumatoid factor levels (r = 0.431, p = 0.0040), but not with matrix metalloproteinase-3, erythrocyte sedimentation rate, and C-reactive protein. Conclusion. CCL18/PARC was highly expressed in RA articular cartilage and synovial tissue compared with OA samples. Our data indicated that CCL18/PARC levels are not related to the conditions of generalized inflammation, but are related to the pathogenesis of RA.",
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AU - Momohara, Shigeki

AU - Okamoto, Hiroshi

AU - Iwamoto, Takuji

AU - Mizumura, Tamao

AU - Ikari, Katsunori

AU - Kawaguchi, Yasushi

AU - Takeuchi, Masahiro

AU - Kamatani, Naoyuki

AU - Tomatsu, Taisuke

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N2 - Objective. We studied the role of CCL18/pulmonary and activation-regulated chemokine (PARC) in rheumatoid arthritis (RA). Methods. Human cartilage tissues and synovial membranes were obtained from patients with RA and with osteoarthritis (OA). Sera samples were obtained from RA patients, OA patients, healthy controls, and patients with flu, and synovial fluid (SF) from patients with RA and OA. Real-time PCR was performed with RNA from cartilage samples. Immunohistochemical analysis of CCL18/PARC was done with RA and OA cartilage and synovial tissue. Levels of CCL18/PARC in serum and SF were evaluated by ELISA. Results. CCL18/PARC mRNA was expressed at significantly higher levels in RA cartilage than in OA (p = 0.0001) and control (p < 0.0001) samples. CCL18/PARC mRNA expression was much higher in RA synovial membrane than OA samples (p = 0.0001). All RA cartilage and synovial tissue samples exhibited medium to strong staining for CCL18/PARC. Serum levels of CCL18/PARC were higher in RA patients (156.21 ± 125.73 ng/ml, n = 71) than in OA patients (64.54 ± 40.90 ng/ml, n = 12) and controls (28.04 ± 10.96 ng/ml, n = 20). Levels of CCL18/PARC in RA SF (275.20 ± 228.16 ng/ml, n = 15) were higher than in OA (33.13 ± 14.84 ng/ml, n = 6; p = 0.0198). CCL18/PARC levels correlated significantly with rheumatoid factor levels (r = 0.431, p = 0.0040), but not with matrix metalloproteinase-3, erythrocyte sedimentation rate, and C-reactive protein. Conclusion. CCL18/PARC was highly expressed in RA articular cartilage and synovial tissue compared with OA samples. Our data indicated that CCL18/PARC levels are not related to the conditions of generalized inflammation, but are related to the pathogenesis of RA.

AB - Objective. We studied the role of CCL18/pulmonary and activation-regulated chemokine (PARC) in rheumatoid arthritis (RA). Methods. Human cartilage tissues and synovial membranes were obtained from patients with RA and with osteoarthritis (OA). Sera samples were obtained from RA patients, OA patients, healthy controls, and patients with flu, and synovial fluid (SF) from patients with RA and OA. Real-time PCR was performed with RNA from cartilage samples. Immunohistochemical analysis of CCL18/PARC was done with RA and OA cartilage and synovial tissue. Levels of CCL18/PARC in serum and SF were evaluated by ELISA. Results. CCL18/PARC mRNA was expressed at significantly higher levels in RA cartilage than in OA (p = 0.0001) and control (p < 0.0001) samples. CCL18/PARC mRNA expression was much higher in RA synovial membrane than OA samples (p = 0.0001). All RA cartilage and synovial tissue samples exhibited medium to strong staining for CCL18/PARC. Serum levels of CCL18/PARC were higher in RA patients (156.21 ± 125.73 ng/ml, n = 71) than in OA patients (64.54 ± 40.90 ng/ml, n = 12) and controls (28.04 ± 10.96 ng/ml, n = 20). Levels of CCL18/PARC in RA SF (275.20 ± 228.16 ng/ml, n = 15) were higher than in OA (33.13 ± 14.84 ng/ml, n = 6; p = 0.0198). CCL18/PARC levels correlated significantly with rheumatoid factor levels (r = 0.431, p = 0.0040), but not with matrix metalloproteinase-3, erythrocyte sedimentation rate, and C-reactive protein. Conclusion. CCL18/PARC was highly expressed in RA articular cartilage and synovial tissue compared with OA samples. Our data indicated that CCL18/PARC levels are not related to the conditions of generalized inflammation, but are related to the pathogenesis of RA.

KW - Cartilage

KW - CCL18/CC chemokine

KW - Pulmonary and activation-regulated chemokine

KW - Rheumatoid arthritis

KW - Synovial tissue

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