High-contrast fluorescence imaging based on the polarization dependence of the fluorescence enhancement using an optical interference mirror slide

Mitsuru Yasuda, Takuo Akimoto

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

High-contrast fluorescence imaging using an optical interference mirror (OIM) slide that enhances the fluorescence from a fluorophore located on top of the OIM surface is reported. To enhance the fluorescence and reduce the background light of the OIM, transverse-electric-polarized excitation light was used as incident light, and the transverse-magnetic-polarized fluorescence signal was detected. As a result, an approximate 100-fold improvement in the signal-to-noise ratio was achieved through a 13-fold enhancement of the fluorescence signal and an 8-fold reduction of the background light.

Original languageEnglish
Pages (from-to)139-143
Number of pages5
Journalanalytical sciences
Volume31
Issue number3
DOIs
Publication statusPublished - 2015 Jan 1
Externally publishedYes

Keywords

  • Biochip
  • Enhanced fluorescence
  • Polarization

ASJC Scopus subject areas

  • Analytical Chemistry

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