TY - JOUR
T1 - High-contrast fluorescence microscopy for a biomolecular analysis based on polarization techniques using an optical interference mirror slide
AU - Yasuda, Mitsuru
AU - Akimoto, Takuo
N1 - Publisher Copyright:
© 2016 by the authors.
PY - 2014
Y1 - 2014
N2 - Fluorescence microscopy with an improved contrast for fluorescence images is developed using an optical interference mirror (OIM) slide, which can enhance the fluorescence from a fluorophore as a result of the double interference of the excitation light and emission light. To improve the contrast of a fluorescence image using an OIM slide, a linearly-polarized excitation light was employed, and the fluorescence emission polarized perpendicular to the polarization of the excitation light was detected. The image contrast with this optical system was improved 110-fold for rhodamine B spotted on the OIM, in comparison with a glass slide using a general fluorescence microscopy optical system. Moreover, a 24-fold improvement of the image contrast was achieved for the detection of Cy3-labeled streptavidin bound to immobilize biotin.
AB - Fluorescence microscopy with an improved contrast for fluorescence images is developed using an optical interference mirror (OIM) slide, which can enhance the fluorescence from a fluorophore as a result of the double interference of the excitation light and emission light. To improve the contrast of a fluorescence image using an OIM slide, a linearly-polarized excitation light was employed, and the fluorescence emission polarized perpendicular to the polarization of the excitation light was detected. The image contrast with this optical system was improved 110-fold for rhodamine B spotted on the OIM, in comparison with a glass slide using a general fluorescence microscopy optical system. Moreover, a 24-fold improvement of the image contrast was achieved for the detection of Cy3-labeled streptavidin bound to immobilize biotin.
KW - Biochip
KW - Fluorescence enhancement
KW - Fluorescence microscopy
UR - http://www.scopus.com/inward/record.url?scp=84986218397&partnerID=8YFLogxK
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U2 - 10.3390/bios4040513
DO - 10.3390/bios4040513
M3 - Article
AN - SCOPUS:84986218397
VL - 4
SP - 513
EP - 522
JO - Biosensors
JF - Biosensors
SN - 2079-6374
IS - 4
ER -