High-contrast fluorescence microscopy for a biomolecular analysis based on polarization techniques using an optical interference mirror slide

Mitsuru Yasuda, Takuo Akimoto

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Fluorescence microscopy with an improved contrast for fluorescence images is developed using an optical interference mirror (OIM) slide, which can enhance the fluorescence from a fluorophore as a result of the double interference of the excitation light and emission light. To improve the contrast of a fluorescence image using an OIM slide, a linearly-polarized excitation light was employed, and the fluorescence emission polarized perpendicular to the polarization of the excitation light was detected. The image contrast with this optical system was improved 110-fold for rhodamine B spotted on the OIM, in comparison with a glass slide using a general fluorescence microscopy optical system. Moreover, a 24-fold improvement of the image contrast was achieved for the detection of Cy3-labeled streptavidin bound to immobilize biotin.

Original languageEnglish
Pages (from-to)513-522
Number of pages10
JournalBiosensors
Volume4
Issue number4
DOIs
Publication statusPublished - 2014

Keywords

  • Biochip
  • Fluorescence enhancement
  • Fluorescence microscopy

ASJC Scopus subject areas

  • Clinical Biochemistry

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