High correlation in antibody titers between the Sabin-Feldman dye test and an enzyme-linked immunosorbent assay detecting immunoglobulin G antibodies to the nucleoside triphosphate hydrolase of Toxoplasma gondii

T. Asai, F. Mizuno, S. Kojima, T. Takeuchi, A. Kobayashi, Y. Suzuki

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

An enzyme-linked immunosorbent assay to detect immunoglobulin G antibodies against nucleoside triphosphate hydrolase, which is a specific and dominant antigen of Toxoplasma gondii, was developed, and the sensitivity and specificity of the test were compared with those of the Sabin-Feldman dye test. One hundred percent agreement was observed in comparative study between those tests on 37 positive and 50 negative human sera. Antibody titers in the enzyme-linked immunosorbent assay test, which were expressed as the reciprocal of the highest positive dilution of serum, were just 100 times those in the dye test on 81% (30 of 37) of the positive sera.

Original languageEnglish
Pages (from-to)1291-1293
Number of pages3
JournalJournal of Clinical Microbiology
Volume30
Issue number5
Publication statusPublished - 1992
Externally publishedYes

Fingerprint

N-Glycosyl Hydrolases
Toxoplasma
Coloring Agents
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Antibodies
Serum
Antigens
Sensitivity and Specificity
triphosphoric acid

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

High correlation in antibody titers between the Sabin-Feldman dye test and an enzyme-linked immunosorbent assay detecting immunoglobulin G antibodies to the nucleoside triphosphate hydrolase of Toxoplasma gondii. / Asai, T.; Mizuno, F.; Kojima, S.; Takeuchi, T.; Kobayashi, A.; Suzuki, Y.

In: Journal of Clinical Microbiology, Vol. 30, No. 5, 1992, p. 1291-1293.

Research output: Contribution to journalArticle

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AU - Suzuki, Y.

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