Abstract
We have developed a new procedure for the selective determination of β1-3 and β1-4 galactosyltransferases with Lc3Cer as the substrate and the microsomes of fetal and adult porcine livers as the enzyme sources. This method was based on the detection of such products as Lc4Cer for β1-3 galactosyltransferase (β1-3GT) and nLc4Cer for β1-4 galactosyltransferase (β1-4GT), with monoclonal anti-Lc4Cer and anti-nLc4Cer antibodies, respectively. This method thus enabled us to differentiate the activity of β1-BGT from that of β1-4GT with a high degree of sensitivity. The method was then used to determine the activities of both enzymes in human gynecological carcinoma-derived cells. Four of the five cell lines derived from uterine endometrial cancer expressed significantly high levels of specific activity of β1-3GT among the cell lines examined, while their β1-4GT activities were less than 20% of that for β1-3GT in the endometrial carcinoma-derived cells. On the other hand, a higher specific activity of β1-4GT than that of β1-3GT was detected in the cell lines derived from uterine cervical and ovarian cancers. These findings were thus found to correlate closely with the rate of expression of Lc4Cer- and nLc4Cer-based carbohydrate chains in the cell lines based on the results staining.
| Original language | English |
|---|---|
| Pages (from-to) | 669-677 |
| Number of pages | 9 |
| Journal | Japanese Journal of Cancer Research |
| Volume | 88 |
| Issue number | 7 |
| Publication status | Published - 1997 Jul |
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Keywords
- Carbohydrate chain
- ELISA
- Galactosyltransferase
- TLC-immunostaining
- Utrine endometrial cancer
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
High expression of uridine diphosphate-galactose : Lc3Cer β1-3 galactosyltransferase in human uterine endometrial cancer-derived cells as measured by enzyme-linked immunosorbent assay and thin-layer chromatography-immunostaining. / Yoshiki, Junko; Kubushiro, Kaneyuki; Tsukazaki, Katsumi; Udagawa, Yasuhiro; Nozawa, Shiro; Iwamori, Masao.
In: Japanese Journal of Cancer Research, Vol. 88, No. 7, 07.1997, p. 669-677.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - High expression of uridine diphosphate-galactose
T2 - Lc3Cer β1-3 galactosyltransferase in human uterine endometrial cancer-derived cells as measured by enzyme-linked immunosorbent assay and thin-layer chromatography-immunostaining
AU - Yoshiki, Junko
AU - Kubushiro, Kaneyuki
AU - Tsukazaki, Katsumi
AU - Udagawa, Yasuhiro
AU - Nozawa, Shiro
AU - Iwamori, Masao
PY - 1997/7
Y1 - 1997/7
N2 - We have developed a new procedure for the selective determination of β1-3 and β1-4 galactosyltransferases with Lc3Cer as the substrate and the microsomes of fetal and adult porcine livers as the enzyme sources. This method was based on the detection of such products as Lc4Cer for β1-3 galactosyltransferase (β1-3GT) and nLc4Cer for β1-4 galactosyltransferase (β1-4GT), with monoclonal anti-Lc4Cer and anti-nLc4Cer antibodies, respectively. This method thus enabled us to differentiate the activity of β1-BGT from that of β1-4GT with a high degree of sensitivity. The method was then used to determine the activities of both enzymes in human gynecological carcinoma-derived cells. Four of the five cell lines derived from uterine endometrial cancer expressed significantly high levels of specific activity of β1-3GT among the cell lines examined, while their β1-4GT activities were less than 20% of that for β1-3GT in the endometrial carcinoma-derived cells. On the other hand, a higher specific activity of β1-4GT than that of β1-3GT was detected in the cell lines derived from uterine cervical and ovarian cancers. These findings were thus found to correlate closely with the rate of expression of Lc4Cer- and nLc4Cer-based carbohydrate chains in the cell lines based on the results staining.
AB - We have developed a new procedure for the selective determination of β1-3 and β1-4 galactosyltransferases with Lc3Cer as the substrate and the microsomes of fetal and adult porcine livers as the enzyme sources. This method was based on the detection of such products as Lc4Cer for β1-3 galactosyltransferase (β1-3GT) and nLc4Cer for β1-4 galactosyltransferase (β1-4GT), with monoclonal anti-Lc4Cer and anti-nLc4Cer antibodies, respectively. This method thus enabled us to differentiate the activity of β1-BGT from that of β1-4GT with a high degree of sensitivity. The method was then used to determine the activities of both enzymes in human gynecological carcinoma-derived cells. Four of the five cell lines derived from uterine endometrial cancer expressed significantly high levels of specific activity of β1-3GT among the cell lines examined, while their β1-4GT activities were less than 20% of that for β1-3GT in the endometrial carcinoma-derived cells. On the other hand, a higher specific activity of β1-4GT than that of β1-3GT was detected in the cell lines derived from uterine cervical and ovarian cancers. These findings were thus found to correlate closely with the rate of expression of Lc4Cer- and nLc4Cer-based carbohydrate chains in the cell lines based on the results staining.
KW - Carbohydrate chain
KW - ELISA
KW - Galactosyltransferase
KW - TLC-immunostaining
KW - Utrine endometrial cancer
UR - http://www.scopus.com/inward/record.url?scp=0030847457&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030847457&partnerID=8YFLogxK
M3 - Article
C2 - 9310140
AN - SCOPUS:0030847457
VL - 88
SP - 669
EP - 677
JO - Cancer Science
JF - Cancer Science
SN - 1347-9032
IS - 7
ER -