High expression of uridine diphosphate-galactose: Lc3Cer β1-3 galactosyltransferase in human uterine endometrial cancer-derived cells as measured by enzyme-linked immunosorbent assay and thin-layer chromatography-immunostaining

Junko Yoshiki, Kaneyuki Kubushiro, Katsumi Tsukazaki, Yasuhiro Udagawa, Shiro Nozawa, Masao Iwamori

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have developed a new procedure for the selective determination of β1-3 and β1-4 galactosyltransferases with Lc3Cer as the substrate and the microsomes of fetal and adult porcine livers as the enzyme sources. This method was based on the detection of such products as Lc4Cer for β1-3 galactosyltransferase (β1-3GT) and nLc4Cer for β1-4 galactosyltransferase (β1-4GT), with monoclonal anti-Lc4Cer and anti-nLc4Cer antibodies, respectively. This method thus enabled us to differentiate the activity of β1-BGT from that of β1-4GT with a high degree of sensitivity. The method was then used to determine the activities of both enzymes in human gynecological carcinoma-derived cells. Four of the five cell lines derived from uterine endometrial cancer expressed significantly high levels of specific activity of β1-3GT among the cell lines examined, while their β1-4GT activities were less than 20% of that for β1-3GT in the endometrial carcinoma-derived cells. On the other hand, a higher specific activity of β1-4GT than that of β1-3GT was detected in the cell lines derived from uterine cervical and ovarian cancers. These findings were thus found to correlate closely with the rate of expression of Lc4Cer- and nLc4Cer-based carbohydrate chains in the cell lines based on the results staining.

Original languageEnglish
Pages (from-to)669-677
Number of pages9
JournalJapanese Journal of Cancer Research
Volume88
Issue number7
Publication statusPublished - 1997 Jul

Fingerprint

Uridine Diphosphate Galactose
Galactosyltransferases
Uterine Neoplasms
Endometrial Neoplasms
Thin Layer Chromatography
Enzyme-Linked Immunosorbent Assay
Cell Line
Enzymes
Microsomes
Uterine Cervical Neoplasms
Ovarian Neoplasms
Anti-Idiotypic Antibodies
Swine
Carbohydrates
Staining and Labeling
Carcinoma

Keywords

  • Carbohydrate chain
  • ELISA
  • Galactosyltransferase
  • TLC-immunostaining
  • Utrine endometrial cancer

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

High expression of uridine diphosphate-galactose : Lc3Cer β1-3 galactosyltransferase in human uterine endometrial cancer-derived cells as measured by enzyme-linked immunosorbent assay and thin-layer chromatography-immunostaining. / Yoshiki, Junko; Kubushiro, Kaneyuki; Tsukazaki, Katsumi; Udagawa, Yasuhiro; Nozawa, Shiro; Iwamori, Masao.

In: Japanese Journal of Cancer Research, Vol. 88, No. 7, 07.1997, p. 669-677.

Research output: Contribution to journalArticle

@article{f7852803d8f94f868d7e2c97a65f4b48,
title = "High expression of uridine diphosphate-galactose: Lc3Cer β1-3 galactosyltransferase in human uterine endometrial cancer-derived cells as measured by enzyme-linked immunosorbent assay and thin-layer chromatography-immunostaining",
abstract = "We have developed a new procedure for the selective determination of β1-3 and β1-4 galactosyltransferases with Lc3Cer as the substrate and the microsomes of fetal and adult porcine livers as the enzyme sources. This method was based on the detection of such products as Lc4Cer for β1-3 galactosyltransferase (β1-3GT) and nLc4Cer for β1-4 galactosyltransferase (β1-4GT), with monoclonal anti-Lc4Cer and anti-nLc4Cer antibodies, respectively. This method thus enabled us to differentiate the activity of β1-BGT from that of β1-4GT with a high degree of sensitivity. The method was then used to determine the activities of both enzymes in human gynecological carcinoma-derived cells. Four of the five cell lines derived from uterine endometrial cancer expressed significantly high levels of specific activity of β1-3GT among the cell lines examined, while their β1-4GT activities were less than 20{\%} of that for β1-3GT in the endometrial carcinoma-derived cells. On the other hand, a higher specific activity of β1-4GT than that of β1-3GT was detected in the cell lines derived from uterine cervical and ovarian cancers. These findings were thus found to correlate closely with the rate of expression of Lc4Cer- and nLc4Cer-based carbohydrate chains in the cell lines based on the results staining.",
keywords = "Carbohydrate chain, ELISA, Galactosyltransferase, TLC-immunostaining, Utrine endometrial cancer",
author = "Junko Yoshiki and Kaneyuki Kubushiro and Katsumi Tsukazaki and Yasuhiro Udagawa and Shiro Nozawa and Masao Iwamori",
year = "1997",
month = "7",
language = "English",
volume = "88",
pages = "669--677",
journal = "Cancer Science",
issn = "1347-9032",
publisher = "Wiley-Blackwell",
number = "7",

}

TY - JOUR

T1 - High expression of uridine diphosphate-galactose

T2 - Lc3Cer β1-3 galactosyltransferase in human uterine endometrial cancer-derived cells as measured by enzyme-linked immunosorbent assay and thin-layer chromatography-immunostaining

AU - Yoshiki, Junko

AU - Kubushiro, Kaneyuki

AU - Tsukazaki, Katsumi

AU - Udagawa, Yasuhiro

AU - Nozawa, Shiro

AU - Iwamori, Masao

PY - 1997/7

Y1 - 1997/7

N2 - We have developed a new procedure for the selective determination of β1-3 and β1-4 galactosyltransferases with Lc3Cer as the substrate and the microsomes of fetal and adult porcine livers as the enzyme sources. This method was based on the detection of such products as Lc4Cer for β1-3 galactosyltransferase (β1-3GT) and nLc4Cer for β1-4 galactosyltransferase (β1-4GT), with monoclonal anti-Lc4Cer and anti-nLc4Cer antibodies, respectively. This method thus enabled us to differentiate the activity of β1-BGT from that of β1-4GT with a high degree of sensitivity. The method was then used to determine the activities of both enzymes in human gynecological carcinoma-derived cells. Four of the five cell lines derived from uterine endometrial cancer expressed significantly high levels of specific activity of β1-3GT among the cell lines examined, while their β1-4GT activities were less than 20% of that for β1-3GT in the endometrial carcinoma-derived cells. On the other hand, a higher specific activity of β1-4GT than that of β1-3GT was detected in the cell lines derived from uterine cervical and ovarian cancers. These findings were thus found to correlate closely with the rate of expression of Lc4Cer- and nLc4Cer-based carbohydrate chains in the cell lines based on the results staining.

AB - We have developed a new procedure for the selective determination of β1-3 and β1-4 galactosyltransferases with Lc3Cer as the substrate and the microsomes of fetal and adult porcine livers as the enzyme sources. This method was based on the detection of such products as Lc4Cer for β1-3 galactosyltransferase (β1-3GT) and nLc4Cer for β1-4 galactosyltransferase (β1-4GT), with monoclonal anti-Lc4Cer and anti-nLc4Cer antibodies, respectively. This method thus enabled us to differentiate the activity of β1-BGT from that of β1-4GT with a high degree of sensitivity. The method was then used to determine the activities of both enzymes in human gynecological carcinoma-derived cells. Four of the five cell lines derived from uterine endometrial cancer expressed significantly high levels of specific activity of β1-3GT among the cell lines examined, while their β1-4GT activities were less than 20% of that for β1-3GT in the endometrial carcinoma-derived cells. On the other hand, a higher specific activity of β1-4GT than that of β1-3GT was detected in the cell lines derived from uterine cervical and ovarian cancers. These findings were thus found to correlate closely with the rate of expression of Lc4Cer- and nLc4Cer-based carbohydrate chains in the cell lines based on the results staining.

KW - Carbohydrate chain

KW - ELISA

KW - Galactosyltransferase

KW - TLC-immunostaining

KW - Utrine endometrial cancer

UR - http://www.scopus.com/inward/record.url?scp=0030847457&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030847457&partnerID=8YFLogxK

M3 - Article

C2 - 9310140

AN - SCOPUS:0030847457

VL - 88

SP - 669

EP - 677

JO - Cancer Science

JF - Cancer Science

SN - 1347-9032

IS - 7

ER -