Abstract
High fat diet (HFD) decreases the lifespan of mice, and is a risk factor for several human diseases. Here, we investigated the effects of a HFD on lung epithelial and stem cells and its interaction with aging. Young and old mice were fed with either a standard diet (SD) or a HFD then their trachea and lung were examined for histological changes, inflammation, and mitochondrial function. Their stem cell function was examined using the in vitro organoid/colony forming efficiency (CFE) assay. Aging reduced the number of tracheal basal and alveolar type-2 (AT2) cells. HFD significantly increased the number of AT2 cells. Aging also caused a significant increase in lung inflammation, and HFD caused a similar increase, in young mice. Aging reduced mitochondrial mass and function, and increased reactive oxygen species. In young mice, HFD caused mitochondrial changes similar to the aging-induced changes. Organoid culture of tracheal and lung epithelial cells collected from both young and old HFD-fed mice showed higher CFE compared to SD-fed mice. Switching the HFD to low calorie/fat diet (LCD) efficiently reversed several of the HFD-induced effects. Thus, HFD induces several histological, inflammatory, and functional changes in the lung, and exacerbates the aging-induced lung inflammation and mitochondrial deterioration. LCD can reverse many of the HFD-induced effects.
| Original language | English |
|---|---|
| Pages (from-to) | 25-35 |
| Number of pages | 11 |
| Journal | Stem Cell Research |
| Volume | 33 |
| DOIs | |
| Publication status | Published - 2018 Dec 1 |
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Keywords
- Aging
- Alveolar cells
- Calorie restriction
- High fat diet
- Lung stem cells
- Mitochondria
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology
Cite this
High fat diet activates adult mouse lung stem cells and accelerates several aging-induced effects. / Hegab, Ahmed E.; Ozaki, Mari; Meligy, Fatma Y.; Kagawa, Shizuko; Ishii, Makoto; Betsuyaku, Tomoko.
In: Stem Cell Research, Vol. 33, 01.12.2018, p. 25-35.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - High fat diet activates adult mouse lung stem cells and accelerates several aging-induced effects
AU - Hegab, Ahmed E.
AU - Ozaki, Mari
AU - Meligy, Fatma Y.
AU - Kagawa, Shizuko
AU - Ishii, Makoto
AU - Betsuyaku, Tomoko
PY - 2018/12/1
Y1 - 2018/12/1
N2 - High fat diet (HFD) decreases the lifespan of mice, and is a risk factor for several human diseases. Here, we investigated the effects of a HFD on lung epithelial and stem cells and its interaction with aging. Young and old mice were fed with either a standard diet (SD) or a HFD then their trachea and lung were examined for histological changes, inflammation, and mitochondrial function. Their stem cell function was examined using the in vitro organoid/colony forming efficiency (CFE) assay. Aging reduced the number of tracheal basal and alveolar type-2 (AT2) cells. HFD significantly increased the number of AT2 cells. Aging also caused a significant increase in lung inflammation, and HFD caused a similar increase, in young mice. Aging reduced mitochondrial mass and function, and increased reactive oxygen species. In young mice, HFD caused mitochondrial changes similar to the aging-induced changes. Organoid culture of tracheal and lung epithelial cells collected from both young and old HFD-fed mice showed higher CFE compared to SD-fed mice. Switching the HFD to low calorie/fat diet (LCD) efficiently reversed several of the HFD-induced effects. Thus, HFD induces several histological, inflammatory, and functional changes in the lung, and exacerbates the aging-induced lung inflammation and mitochondrial deterioration. LCD can reverse many of the HFD-induced effects.
AB - High fat diet (HFD) decreases the lifespan of mice, and is a risk factor for several human diseases. Here, we investigated the effects of a HFD on lung epithelial and stem cells and its interaction with aging. Young and old mice were fed with either a standard diet (SD) or a HFD then their trachea and lung were examined for histological changes, inflammation, and mitochondrial function. Their stem cell function was examined using the in vitro organoid/colony forming efficiency (CFE) assay. Aging reduced the number of tracheal basal and alveolar type-2 (AT2) cells. HFD significantly increased the number of AT2 cells. Aging also caused a significant increase in lung inflammation, and HFD caused a similar increase, in young mice. Aging reduced mitochondrial mass and function, and increased reactive oxygen species. In young mice, HFD caused mitochondrial changes similar to the aging-induced changes. Organoid culture of tracheal and lung epithelial cells collected from both young and old HFD-fed mice showed higher CFE compared to SD-fed mice. Switching the HFD to low calorie/fat diet (LCD) efficiently reversed several of the HFD-induced effects. Thus, HFD induces several histological, inflammatory, and functional changes in the lung, and exacerbates the aging-induced lung inflammation and mitochondrial deterioration. LCD can reverse many of the HFD-induced effects.
KW - Aging
KW - Alveolar cells
KW - Calorie restriction
KW - High fat diet
KW - Lung stem cells
KW - Mitochondria
UR - http://www.scopus.com/inward/record.url?scp=85054462872&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85054462872&partnerID=8YFLogxK
U2 - 10.1016/j.scr.2018.10.006
DO - 10.1016/j.scr.2018.10.006
M3 - Article
VL - 33
SP - 25
EP - 35
JO - Stem Cell Research
JF - Stem Cell Research
SN - 1873-5061
ER -