Abstract
Embryonal tumors such as Wilms' tumor (WT), embryonal rhabdomyosarcoma (eRMS) and hepatoblastoma have been thought to have a common pathogenetic mechanism. H19 was found to be inactivated in WT and eRMS either by loss of heterozygosity or by hypermethylation of the maternal allele. We show here that the expression of the H19 gene is inactivated by maternal allelic loss or hypermethylation in 7 out of 8 'sporadic' hepatoblastomas. Furthermore, we analyzed expression of the IGF2 gene. Loss of imprinting of the IGF2 gene was detected and linked to inactivation of the H19 gene in 2 hepatoblastomas. However, 2 sporadic cases demonstrated monoallelic expression of the IGF2 gene with inactivation of the H19 gene. Our results suggest that H19 may play a role as a common imprinted tumor suppressor gene in 'sporadic' hepatoblastomas but may at times work independently of IGF2 expression.
| Original language | English |
|---|---|
| Pages (from-to) | 490-497 |
| Number of pages | 8 |
| Journal | International Journal of Cancer |
| Volume | 82 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1999 |
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ASJC Scopus subject areas
- Cancer Research
- Oncology
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High frequency of inactivation of the imprinted H19 gene in 'sporadic' hepatoblastoma. / Fukuzawa, Ryuji; Umezawa, Akihiro; Ochi, Kensuke; Urano, Fumihiko; Ikeda, Hitoshi; Hata, Jun Ichi.
In: International Journal of Cancer, Vol. 82, No. 4, 1999, p. 490-497.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - High frequency of inactivation of the imprinted H19 gene in 'sporadic' hepatoblastoma
AU - Fukuzawa, Ryuji
AU - Umezawa, Akihiro
AU - Ochi, Kensuke
AU - Urano, Fumihiko
AU - Ikeda, Hitoshi
AU - Hata, Jun Ichi
PY - 1999
Y1 - 1999
N2 - Embryonal tumors such as Wilms' tumor (WT), embryonal rhabdomyosarcoma (eRMS) and hepatoblastoma have been thought to have a common pathogenetic mechanism. H19 was found to be inactivated in WT and eRMS either by loss of heterozygosity or by hypermethylation of the maternal allele. We show here that the expression of the H19 gene is inactivated by maternal allelic loss or hypermethylation in 7 out of 8 'sporadic' hepatoblastomas. Furthermore, we analyzed expression of the IGF2 gene. Loss of imprinting of the IGF2 gene was detected and linked to inactivation of the H19 gene in 2 hepatoblastomas. However, 2 sporadic cases demonstrated monoallelic expression of the IGF2 gene with inactivation of the H19 gene. Our results suggest that H19 may play a role as a common imprinted tumor suppressor gene in 'sporadic' hepatoblastomas but may at times work independently of IGF2 expression.
AB - Embryonal tumors such as Wilms' tumor (WT), embryonal rhabdomyosarcoma (eRMS) and hepatoblastoma have been thought to have a common pathogenetic mechanism. H19 was found to be inactivated in WT and eRMS either by loss of heterozygosity or by hypermethylation of the maternal allele. We show here that the expression of the H19 gene is inactivated by maternal allelic loss or hypermethylation in 7 out of 8 'sporadic' hepatoblastomas. Furthermore, we analyzed expression of the IGF2 gene. Loss of imprinting of the IGF2 gene was detected and linked to inactivation of the H19 gene in 2 hepatoblastomas. However, 2 sporadic cases demonstrated monoallelic expression of the IGF2 gene with inactivation of the H19 gene. Our results suggest that H19 may play a role as a common imprinted tumor suppressor gene in 'sporadic' hepatoblastomas but may at times work independently of IGF2 expression.
UR - http://www.scopus.com/inward/record.url?scp=0032865667&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032865667&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-0215(19990812)82:4<490::AID-IJC4>3.0.CO;2-I
DO - 10.1002/(SICI)1097-0215(19990812)82:4<490::AID-IJC4>3.0.CO;2-I
M3 - Article
C2 - 10404060
AN - SCOPUS:0032865667
VL - 82
SP - 490
EP - 497
JO - International Journal of Cancer
JF - International Journal of Cancer
SN - 0020-7136
IS - 4
ER -