High-LET radiation-induced aberrations in prematurely condensed G2 chromosomes of human fibroblasts

T. Kawata, E. Gotoh, M. Durante, H. Wu, K. George, Y. Furusawa, F. A. Cucinotta

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Purpose: To determine the number of initial chromatid breaks induced by low- or high-LET irradiations, and to compare the kinetics of chromatid break rejoining for radiations of different quality. Material and methods: Exponentially growing human fibroblast cells AG1522 were irradiated with γ-rays, energetic carbon (290 MeV/u), silicon (490 MeV/u) and iron (200 and 600 MeV/u). Chromosomes were prematurely condensed using calyculin A. Chromatid breaks and exchanges in G2 cells were scored. PCC were collected after several post-irradiation incubation times, ranging from 5 to 600 min. Results: The kinetics of chromatid break rejoining following low- or high-LET irradiation consisted of two exponential components representing a rapid and a slow time constant. Chromatid breaks decreased rapidly during the first 10 min after exposure, then continued to decrease at a slower rate. The rejoining kinetics were similar for exposure to each type of radiation. Chromatid exchanges were also formed quickly. Compared to low-LET radiation, isochromatid breaks were produced more frequently and the proportion of unrejoined breaks was higher for high-LET radiation. Conclusions: Compared with γ-rays, isochromatid breaks were observed more frequently in high-LET irradiated samples, suggesting that an increase in isochromatid breaks is a signature of high-LET radiation exposure.

Original languageEnglish
Pages (from-to)929-937
Number of pages9
JournalInternational Journal of Radiation Biology
Volume76
Issue number7
Publication statusPublished - 2000

Fingerprint

Linear Energy Transfer
chromatids
chromosomes
fibroblasts
Human Chromosomes
Fibroblasts
Chromosomes
Aberrations
Chromatids
aberration
Radiation
radiation
irradiation
Irradiation
rays
kinetics
Kinetics
radiation dosage
time constant
proportion

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Nuclear Energy and Engineering
  • Radiation

Cite this

Kawata, T., Gotoh, E., Durante, M., Wu, H., George, K., Furusawa, Y., & Cucinotta, F. A. (2000). High-LET radiation-induced aberrations in prematurely condensed G2 chromosomes of human fibroblasts. International Journal of Radiation Biology, 76(7), 929-937.

High-LET radiation-induced aberrations in prematurely condensed G2 chromosomes of human fibroblasts. / Kawata, T.; Gotoh, E.; Durante, M.; Wu, H.; George, K.; Furusawa, Y.; Cucinotta, F. A.

In: International Journal of Radiation Biology, Vol. 76, No. 7, 2000, p. 929-937.

Research output: Contribution to journalArticle

Kawata, T, Gotoh, E, Durante, M, Wu, H, George, K, Furusawa, Y & Cucinotta, FA 2000, 'High-LET radiation-induced aberrations in prematurely condensed G2 chromosomes of human fibroblasts', International Journal of Radiation Biology, vol. 76, no. 7, pp. 929-937.
Kawata, T. ; Gotoh, E. ; Durante, M. ; Wu, H. ; George, K. ; Furusawa, Y. ; Cucinotta, F. A. / High-LET radiation-induced aberrations in prematurely condensed G2 chromosomes of human fibroblasts. In: International Journal of Radiation Biology. 2000 ; Vol. 76, No. 7. pp. 929-937.
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