TY - JOUR
T1 - High-level expression of Bacillus thuringiensis phosphatidylinositol-specific phospholipase C by the Bacillus brevis host-vector system
AU - Kobayashi, Tomoko
AU - Tamura, Hiro Omi
AU - Taguchi, Ryo
AU - Udaka, Shigezo
AU - Ikezawa, Hiroh
PY - 1996
Y1 - 1996
N2 - We succeeded in hyperproduction of Bacillus thuringiensis phosphatidylinositol-specific phospholipase C (PIPLC), using a Bacillus brevis 47 expression system. The recombinant B. thuringiensis PIPLC was expressed under the control of the middle wall protein gene promoter in B. brevis expression vector pNU211. A large amount of recombinant PIPLC (0.4 g per liter culture) was secreted into the medium as a mature enzyme, and the enzymatic properties of purified recombinant PIPLC were similar to those of the enzyme from wild-type B. thuringiensis. This system provides a useful approach to the three-dimensional structure-function relationship of PIPLC.
AB - We succeeded in hyperproduction of Bacillus thuringiensis phosphatidylinositol-specific phospholipase C (PIPLC), using a Bacillus brevis 47 expression system. The recombinant B. thuringiensis PIPLC was expressed under the control of the middle wall protein gene promoter in B. brevis expression vector pNU211. A large amount of recombinant PIPLC (0.4 g per liter culture) was secreted into the medium as a mature enzyme, and the enzymatic properties of purified recombinant PIPLC were similar to those of the enzyme from wild-type B. thuringiensis. This system provides a useful approach to the three-dimensional structure-function relationship of PIPLC.
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U2 - 10.7883/yoken1952.49.103
DO - 10.7883/yoken1952.49.103
M3 - Article
C2 - 8950642
AN - SCOPUS:0029811914
SN - 1344-6304
VL - 49
SP - 103
EP - 112
JO - Japanese medical journal
JF - Japanese medical journal
IS - 3
ER -