High-level expression of Bacillus thuringiensis phosphatidylinositol-specific phospholipase C by the Bacillus brevis host-vector system

Tomoko Kobayashi, Hiroomi Tamura, Ryo Taguchi, Shigezo Udaka, Hiroh Ikezawa

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We succeeded in hyperproduction of Bacillus thuringiensis phosphatidylinositol-specific phospholipase C (PIPLC), using a Bacillus brevis 47 expression system. The recombinant B. thuringiensis PIPLC was expressed under the control of the middle wall protein gene promoter in B. brevis expression vector pNU211. A large amount of recombinant PIPLC (0.4 g per liter culture) was secreted into the medium as a mature enzyme, and the enzymatic properties of purified recombinant PIPLC were similar to those of the enzyme from wild-type B. thuringiensis. This system provides a useful approach to the three-dimensional structure-function relationship of PIPLC.

Original languageEnglish
Pages (from-to)103-112
Number of pages10
JournalJapanese Journal of Medical Science and Biology
Volume49
Issue number3
Publication statusPublished - 1996
Externally publishedYes

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Phosphoinositide Phospholipase C
Bacillus thuringiensis
Bacilli
Bacillus
Enzymes
Proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

High-level expression of Bacillus thuringiensis phosphatidylinositol-specific phospholipase C by the Bacillus brevis host-vector system. / Kobayashi, Tomoko; Tamura, Hiroomi; Taguchi, Ryo; Udaka, Shigezo; Ikezawa, Hiroh.

In: Japanese Journal of Medical Science and Biology, Vol. 49, No. 3, 1996, p. 103-112.

Research output: Contribution to journalArticle

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