High-level expression of Bacillus thuringiensis phosphatidylinositol-specific phospholipase C by the Bacillus brevis host-vector system

Tomoko Kobayashi, Hiro Omi Tamura, Ryo Taguchi, Shigezo Udaka, Hiroh Ikezawa

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8 Citations (Scopus)

Abstract

We succeeded in hyperproduction of Bacillus thuringiensis phosphatidylinositol-specific phospholipase C (PIPLC), using a Bacillus brevis 47 expression system. The recombinant B. thuringiensis PIPLC was expressed under the control of the middle wall protein gene promoter in B. brevis expression vector pNU211. A large amount of recombinant PIPLC (0.4 g per liter culture) was secreted into the medium as a mature enzyme, and the enzymatic properties of purified recombinant PIPLC were similar to those of the enzyme from wild-type B. thuringiensis. This system provides a useful approach to the three-dimensional structure-function relationship of PIPLC.

Original languageEnglish
Pages (from-to)103-112
Number of pages10
JournalJapanese Journal of Medical Science and Biology
Volume49
Issue number3
DOIs
Publication statusPublished - 1996 Jan 1
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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