High-performance liquid chromatographic analysis of ginsenosides in Panax ginseng extracts using glass-ODS column

H. Kanazawa, Y. Nagata, Y. Matsushima, M. Tomoda, N. Takai

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Octadecyl-porous glass was prepared and used as the packing for reversed-phase high-performance liquid chromatography. A mixture of ginsenosides, saponins of ginseng was analyzed with detection at 203 nm. Ginsenosides Rf, Rg2, Rb1, Rc, Rb2, and Rd were separated with acetonitrile-water (27.5:72.5) as the mobile phase. A well-resoluted chromatogram of ginsenosides Ro, Rg1 and Re was also obtained with acetonitrile-water (16.5:83.5). The whole separation was achieved in 12 min with a flowrate of 1 ml/min. Calibration curves of ginsenosides Rb1, Rc, Rb2, Rd, Rg1 and Re were linear up to 5 μg. It can be concluded that the rapid and accurate analysis of ginsenosides is possible by the described method.

Original languageEnglish
Pages (from-to)517-519
Number of pages3
JournalChromatographia
Volume24
Issue number1
DOIs
Publication statusPublished - 1987 Dec 1

Keywords

  • Column liquid chromatography
  • Ginsenosides
  • Octadecylsilyl-porous glass
  • Panax ginseng

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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