Abstract
Octadecyl-porous glass was prepared and used as the packing for reversed-phase high-performance liquid chromatography. A mixture of ginsenosides, saponins of ginseng was analyzed with detection at 203 nm. Ginsenosides Rf, Rg2, Rb1, Rc, Rb2, and Rd were separated with acetonitrile-water (27.5:72.5) as the mobile phase. A well-resoluted chromatogram of ginsenosides Ro, Rg1 and Re was also obtained with acetonitrile-water (16.5:83.5). The whole separation was achieved in 12 min with a flowrate of 1 ml/min. Calibration curves of ginsenosides Rb1, Rc, Rb2, Rd, Rg1 and Re were linear up to 5 μg. It can be concluded that the rapid and accurate analysis of ginsenosides is possible by the described method.
Original language | English |
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Pages (from-to) | 517-519 |
Number of pages | 3 |
Journal | Chromatographia |
Volume | 24 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1987 Dec 1 |
Keywords
- Column liquid chromatography
- Ginsenosides
- Octadecylsilyl-porous glass
- Panax ginseng
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Clinical Biochemistry
- Organic Chemistry