Abstract
Octadecyl-porous glass was prepared and used as the packing for reversed-phase high-performance liquid chromatography. A mixture of ginsenosides, saponins of ginseng was analyzed with detection at 203 nm. Ginsenosides Rf, Rg2, Rb1, Rc, Rb2, and Rd were separated with acetonitrile-water (27.5:72.5) as the mobile phase. A well-resoluted chromatogram of ginsenosides Ro, Rg1 and Re was also obtained with acetonitrile-water (16.5:83.5). The whole separation was achieved in 12 min with a flowrate of 1 ml/min. Calibration curves of ginsenosides Rb1, Rc, Rb2, Rd, Rg1 and Re were linear up to 5 μg. It can be concluded that the rapid and accurate analysis of ginsenosides is possible by the described method.
| Original language | English |
|---|---|
| Pages (from-to) | 517-519 |
| Number of pages | 3 |
| Journal | Chromatographia |
| Volume | 24 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1987 Dec |
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Keywords
- Column liquid chromatography
- Ginsenosides
- Octadecylsilyl-porous glass
- Panax ginseng
ASJC Scopus subject areas
- Analytical Chemistry
- Clinical Biochemistry
- Organic Chemistry
- Biochemistry
Cite this
High-performance liquid chromatographic analysis of ginsenosides in Panax ginseng extracts using glass-ODS column. / Kanazawa, Hideko; Itoh, Yoshiko; Matsushima, Y.; Tomoda, M.; Takai, N.
In: Chromatographia, Vol. 24, No. 1, 12.1987, p. 517-519.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - High-performance liquid chromatographic analysis of ginsenosides in Panax ginseng extracts using glass-ODS column
AU - Kanazawa, Hideko
AU - Itoh, Yoshiko
AU - Matsushima, Y.
AU - Tomoda, M.
AU - Takai, N.
PY - 1987/12
Y1 - 1987/12
N2 - Octadecyl-porous glass was prepared and used as the packing for reversed-phase high-performance liquid chromatography. A mixture of ginsenosides, saponins of ginseng was analyzed with detection at 203 nm. Ginsenosides Rf, Rg2, Rb1, Rc, Rb2, and Rd were separated with acetonitrile-water (27.5:72.5) as the mobile phase. A well-resoluted chromatogram of ginsenosides Ro, Rg1 and Re was also obtained with acetonitrile-water (16.5:83.5). The whole separation was achieved in 12 min with a flowrate of 1 ml/min. Calibration curves of ginsenosides Rb1, Rc, Rb2, Rd, Rg1 and Re were linear up to 5 μg. It can be concluded that the rapid and accurate analysis of ginsenosides is possible by the described method.
AB - Octadecyl-porous glass was prepared and used as the packing for reversed-phase high-performance liquid chromatography. A mixture of ginsenosides, saponins of ginseng was analyzed with detection at 203 nm. Ginsenosides Rf, Rg2, Rb1, Rc, Rb2, and Rd were separated with acetonitrile-water (27.5:72.5) as the mobile phase. A well-resoluted chromatogram of ginsenosides Ro, Rg1 and Re was also obtained with acetonitrile-water (16.5:83.5). The whole separation was achieved in 12 min with a flowrate of 1 ml/min. Calibration curves of ginsenosides Rb1, Rc, Rb2, Rd, Rg1 and Re were linear up to 5 μg. It can be concluded that the rapid and accurate analysis of ginsenosides is possible by the described method.
KW - Column liquid chromatography
KW - Ginsenosides
KW - Octadecylsilyl-porous glass
KW - Panax ginseng
UR - http://www.scopus.com/inward/record.url?scp=0000557312&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0000557312&partnerID=8YFLogxK
U2 - 10.1007/BF02688537
DO - 10.1007/BF02688537
M3 - Article
AN - SCOPUS:0000557312
VL - 24
SP - 517
EP - 519
JO - Chromatographia
JF - Chromatographia
SN - 0009-5893
IS - 1
ER -