High-performance liquid chromatographic analysis of ginsenosides in Panax ginseng extracts using glass-ODS column

Hideko Kanazawa, Yoshiko Itoh, Y. Matsushima, M. Tomoda, N. Takai

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Octadecyl-porous glass was prepared and used as the packing for reversed-phase high-performance liquid chromatography. A mixture of ginsenosides, saponins of ginseng was analyzed with detection at 203 nm. Ginsenosides Rf, Rg2, Rb1, Rc, Rb2, and Rd were separated with acetonitrile-water (27.5:72.5) as the mobile phase. A well-resoluted chromatogram of ginsenosides Ro, Rg1 and Re was also obtained with acetonitrile-water (16.5:83.5). The whole separation was achieved in 12 min with a flowrate of 1 ml/min. Calibration curves of ginsenosides Rb1, Rc, Rb2, Rd, Rg1 and Re were linear up to 5 μg. It can be concluded that the rapid and accurate analysis of ginsenosides is possible by the described method.

Original languageEnglish
Pages (from-to)517-519
Number of pages3
JournalChromatographia
Volume24
Issue number1
DOIs
Publication statusPublished - 1987 Dec

Fingerprint

Ginsenosides
Panax
ginsenoside Rg2
High performance liquid chromatography
Glass
High Pressure Liquid Chromatography
Water
Saponins
Reverse-Phase Chromatography
Calibration
acetonitrile
ginsenoside Rb1
ginsenoside Rc
ginsenoside Rf
ginsenoside Re
ginsenoside Rg1
ginsenoside Ro

Keywords

  • Column liquid chromatography
  • Ginsenosides
  • Octadecylsilyl-porous glass
  • Panax ginseng

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Organic Chemistry
  • Biochemistry

Cite this

High-performance liquid chromatographic analysis of ginsenosides in Panax ginseng extracts using glass-ODS column. / Kanazawa, Hideko; Itoh, Yoshiko; Matsushima, Y.; Tomoda, M.; Takai, N.

In: Chromatographia, Vol. 24, No. 1, 12.1987, p. 517-519.

Research output: Contribution to journalArticle

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AU - Takai, N.

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N2 - Octadecyl-porous glass was prepared and used as the packing for reversed-phase high-performance liquid chromatography. A mixture of ginsenosides, saponins of ginseng was analyzed with detection at 203 nm. Ginsenosides Rf, Rg2, Rb1, Rc, Rb2, and Rd were separated with acetonitrile-water (27.5:72.5) as the mobile phase. A well-resoluted chromatogram of ginsenosides Ro, Rg1 and Re was also obtained with acetonitrile-water (16.5:83.5). The whole separation was achieved in 12 min with a flowrate of 1 ml/min. Calibration curves of ginsenosides Rb1, Rc, Rb2, Rd, Rg1 and Re were linear up to 5 μg. It can be concluded that the rapid and accurate analysis of ginsenosides is possible by the described method.

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