High-performance liquid chromatographic analysis of ginsenosides in Panax ginseng extracts using glass-ODS column

H. Kanazawa; Y. Nagata; Y. Matsushima; M. Tomoda; N. Takai

doi:10.1007/BF02688537

High-performance liquid chromatographic analysis of ginsenosides in Panax ginseng extracts using glass-ODS column

H. Kanazawa, Y. Nagata, Y. Matsushima, M. Tomoda, N. Takai

School of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Octadecyl-porous glass was prepared and used as the packing for reversed-phase high-performance liquid chromatography. A mixture of ginsenosides, saponins of ginseng was analyzed with detection at 203 nm. Ginsenosides Rf, Rg₂, Rb₁, Rc, Rb₂, and Rd were separated with acetonitrile-water (27.5:72.5) as the mobile phase. A well-resoluted chromatogram of ginsenosides Ro, Rg₁ and Re was also obtained with acetonitrile-water (16.5:83.5). The whole separation was achieved in 12 min with a flowrate of 1 ml/min. Calibration curves of ginsenosides Rb₁, Rc, Rb₂, Rd, Rg₁ and Re were linear up to 5 μg. It can be concluded that the rapid and accurate analysis of ginsenosides is possible by the described method.

Original language	English
Pages (from-to)	517-519
Number of pages	3
Journal	Chromatographia
Volume	24
Issue number	1
DOIs	https://doi.org/10.1007/BF02688537
Publication status	Published - 1987 Dec 1

Keywords

Column liquid chromatography
Ginsenosides
Octadecylsilyl-porous glass
Panax ginseng

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry
Organic Chemistry

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title = "High-performance liquid chromatographic analysis of ginsenosides in Panax ginseng extracts using glass-ODS column",

abstract = "Octadecyl-porous glass was prepared and used as the packing for reversed-phase high-performance liquid chromatography. A mixture of ginsenosides, saponins of ginseng was analyzed with detection at 203 nm. Ginsenosides Rf, Rg2, Rb1, Rc, Rb2, and Rd were separated with acetonitrile-water (27.5:72.5) as the mobile phase. A well-resoluted chromatogram of ginsenosides Ro, Rg1 and Re was also obtained with acetonitrile-water (16.5:83.5). The whole separation was achieved in 12 min with a flowrate of 1 ml/min. Calibration curves of ginsenosides Rb1, Rc, Rb2, Rd, Rg1 and Re were linear up to 5 μg. It can be concluded that the rapid and accurate analysis of ginsenosides is possible by the described method.",

keywords = "Column liquid chromatography, Ginsenosides, Octadecylsilyl-porous glass, Panax ginseng",

author = "H. Kanazawa and Y. Nagata and Y. Matsushima and M. Tomoda and N. Takai",

year = "1987",

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doi = "10.1007/BF02688537",

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T1 - High-performance liquid chromatographic analysis of ginsenosides in Panax ginseng extracts using glass-ODS column

AU - Kanazawa, H.

AU - Nagata, Y.

AU - Matsushima, Y.

AU - Tomoda, M.

AU - Takai, N.

PY - 1987/12/1

Y1 - 1987/12/1

N2 - Octadecyl-porous glass was prepared and used as the packing for reversed-phase high-performance liquid chromatography. A mixture of ginsenosides, saponins of ginseng was analyzed with detection at 203 nm. Ginsenosides Rf, Rg2, Rb1, Rc, Rb2, and Rd were separated with acetonitrile-water (27.5:72.5) as the mobile phase. A well-resoluted chromatogram of ginsenosides Ro, Rg1 and Re was also obtained with acetonitrile-water (16.5:83.5). The whole separation was achieved in 12 min with a flowrate of 1 ml/min. Calibration curves of ginsenosides Rb1, Rc, Rb2, Rd, Rg1 and Re were linear up to 5 μg. It can be concluded that the rapid and accurate analysis of ginsenosides is possible by the described method.

AB - Octadecyl-porous glass was prepared and used as the packing for reversed-phase high-performance liquid chromatography. A mixture of ginsenosides, saponins of ginseng was analyzed with detection at 203 nm. Ginsenosides Rf, Rg2, Rb1, Rc, Rb2, and Rd were separated with acetonitrile-water (27.5:72.5) as the mobile phase. A well-resoluted chromatogram of ginsenosides Ro, Rg1 and Re was also obtained with acetonitrile-water (16.5:83.5). The whole separation was achieved in 12 min with a flowrate of 1 ml/min. Calibration curves of ginsenosides Rb1, Rc, Rb2, Rd, Rg1 and Re were linear up to 5 μg. It can be concluded that the rapid and accurate analysis of ginsenosides is possible by the described method.

KW - Column liquid chromatography

KW - Ginsenosides

KW - Octadecylsilyl-porous glass

KW - Panax ginseng

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