High-performance liquid chromatographic determination of catecholamine metabolites and 5-hydroxyindoleacetic acid in human urine using a mixed-mode column and an eight-channel electrode electrochemical detector

Fumiko Mashige, Akiyuki Ohkubo, Yoshikazu Matsushima, Maiko Takano, Etsuko Tsuchiya, Hideko Kanazawa, Yoshiko Itoh, Nobuharu Takai, Noriko Shinozuka, Ichiro Sakuma

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

An HPLC system for the simultaneous determination of acidic catecholamine metabolites, related compounds and 5-hydroxyindoleacetic acid (5-HIAA) in human urine was developed. A mixed-mode (C18/anion-exchange) column with isocratic elution using citrate buffer and an eight-channel electrochemical detector were used. Vanilmandelic acid (VMA), 3,4-dihydroxyphenylacetic acid (DOPAC), 4-hydroxy-3-methoxyphenyllactic acid (vanillactic acid, VLA), homovanillic acid (HVA), vanillic acid (VA) and 5-HIAA in urine were determined simultaneously. Detection limits and inter (n = 5) and intra-assay (n = 5) coefficients of variation were satisfactory. The mean of analytical recoveries (n = 3, ±C.V. (%)) were between 97 ± 3.2 (VMA) and 105 ± 4.8 (VA). Correlations between the analytical results for VMA, HVA and 5-HIAA obtained by an established method and the present method were satisfactory. The mean ±2 S.D. of the excretion rates of VMA, DOPAC, VLA, HVA, 5-HIAA and VA in urine from healthy adult volunteers were 0.61-4.36, 0.13-1.02, 0-0.35, 0.67-6.55, 0.50-5.14 and 0-0.55 mg/g creatinine, respectively.

Original languageEnglish
Pages (from-to)63-68
Number of pages6
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume658
Issue number1
DOIs
Publication statusPublished - 1994 Aug 5

Fingerprint

Electrochemical electrodes
Vanilmandelic Acid
Hydroxyindoleacetic Acid
Vanillic Acid
Metabolites
Homovanillic Acid
Catecholamines
Detectors
Liquids
3,4-Dihydroxyphenylacetic Acid
Acids
Citric Acid
Anions
Assays
Creatinine
Buffers
Recovery

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

High-performance liquid chromatographic determination of catecholamine metabolites and 5-hydroxyindoleacetic acid in human urine using a mixed-mode column and an eight-channel electrode electrochemical detector. / Mashige, Fumiko; Ohkubo, Akiyuki; Matsushima, Yoshikazu; Takano, Maiko; Tsuchiya, Etsuko; Kanazawa, Hideko; Itoh, Yoshiko; Takai, Nobuharu; Shinozuka, Noriko; Sakuma, Ichiro.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 658, No. 1, 05.08.1994, p. 63-68.

Research output: Contribution to journalArticle

@article{cd10f4482f104172adf87bf5a5221ad9,
title = "High-performance liquid chromatographic determination of catecholamine metabolites and 5-hydroxyindoleacetic acid in human urine using a mixed-mode column and an eight-channel electrode electrochemical detector",
abstract = "An HPLC system for the simultaneous determination of acidic catecholamine metabolites, related compounds and 5-hydroxyindoleacetic acid (5-HIAA) in human urine was developed. A mixed-mode (C18/anion-exchange) column with isocratic elution using citrate buffer and an eight-channel electrochemical detector were used. Vanilmandelic acid (VMA), 3,4-dihydroxyphenylacetic acid (DOPAC), 4-hydroxy-3-methoxyphenyllactic acid (vanillactic acid, VLA), homovanillic acid (HVA), vanillic acid (VA) and 5-HIAA in urine were determined simultaneously. Detection limits and inter (n = 5) and intra-assay (n = 5) coefficients of variation were satisfactory. The mean of analytical recoveries (n = 3, ±C.V. ({\%})) were between 97 ± 3.2 (VMA) and 105 ± 4.8 (VA). Correlations between the analytical results for VMA, HVA and 5-HIAA obtained by an established method and the present method were satisfactory. The mean ±2 S.D. of the excretion rates of VMA, DOPAC, VLA, HVA, 5-HIAA and VA in urine from healthy adult volunteers were 0.61-4.36, 0.13-1.02, 0-0.35, 0.67-6.55, 0.50-5.14 and 0-0.55 mg/g creatinine, respectively.",
author = "Fumiko Mashige and Akiyuki Ohkubo and Yoshikazu Matsushima and Maiko Takano and Etsuko Tsuchiya and Hideko Kanazawa and Yoshiko Itoh and Nobuharu Takai and Noriko Shinozuka and Ichiro Sakuma",
year = "1994",
month = "8",
day = "5",
doi = "10.1016/0378-4347(94)00227-4",
language = "English",
volume = "658",
pages = "63--68",
journal = "Journal of Chromatography B: Biomedical Sciences and Applications",
issn = "0378-4347",
publisher = "Elsevier BV",
number = "1",

}

TY - JOUR

T1 - High-performance liquid chromatographic determination of catecholamine metabolites and 5-hydroxyindoleacetic acid in human urine using a mixed-mode column and an eight-channel electrode electrochemical detector

AU - Mashige, Fumiko

AU - Ohkubo, Akiyuki

AU - Matsushima, Yoshikazu

AU - Takano, Maiko

AU - Tsuchiya, Etsuko

AU - Kanazawa, Hideko

AU - Itoh, Yoshiko

AU - Takai, Nobuharu

AU - Shinozuka, Noriko

AU - Sakuma, Ichiro

PY - 1994/8/5

Y1 - 1994/8/5

N2 - An HPLC system for the simultaneous determination of acidic catecholamine metabolites, related compounds and 5-hydroxyindoleacetic acid (5-HIAA) in human urine was developed. A mixed-mode (C18/anion-exchange) column with isocratic elution using citrate buffer and an eight-channel electrochemical detector were used. Vanilmandelic acid (VMA), 3,4-dihydroxyphenylacetic acid (DOPAC), 4-hydroxy-3-methoxyphenyllactic acid (vanillactic acid, VLA), homovanillic acid (HVA), vanillic acid (VA) and 5-HIAA in urine were determined simultaneously. Detection limits and inter (n = 5) and intra-assay (n = 5) coefficients of variation were satisfactory. The mean of analytical recoveries (n = 3, ±C.V. (%)) were between 97 ± 3.2 (VMA) and 105 ± 4.8 (VA). Correlations between the analytical results for VMA, HVA and 5-HIAA obtained by an established method and the present method were satisfactory. The mean ±2 S.D. of the excretion rates of VMA, DOPAC, VLA, HVA, 5-HIAA and VA in urine from healthy adult volunteers were 0.61-4.36, 0.13-1.02, 0-0.35, 0.67-6.55, 0.50-5.14 and 0-0.55 mg/g creatinine, respectively.

AB - An HPLC system for the simultaneous determination of acidic catecholamine metabolites, related compounds and 5-hydroxyindoleacetic acid (5-HIAA) in human urine was developed. A mixed-mode (C18/anion-exchange) column with isocratic elution using citrate buffer and an eight-channel electrochemical detector were used. Vanilmandelic acid (VMA), 3,4-dihydroxyphenylacetic acid (DOPAC), 4-hydroxy-3-methoxyphenyllactic acid (vanillactic acid, VLA), homovanillic acid (HVA), vanillic acid (VA) and 5-HIAA in urine were determined simultaneously. Detection limits and inter (n = 5) and intra-assay (n = 5) coefficients of variation were satisfactory. The mean of analytical recoveries (n = 3, ±C.V. (%)) were between 97 ± 3.2 (VMA) and 105 ± 4.8 (VA). Correlations between the analytical results for VMA, HVA and 5-HIAA obtained by an established method and the present method were satisfactory. The mean ±2 S.D. of the excretion rates of VMA, DOPAC, VLA, HVA, 5-HIAA and VA in urine from healthy adult volunteers were 0.61-4.36, 0.13-1.02, 0-0.35, 0.67-6.55, 0.50-5.14 and 0-0.55 mg/g creatinine, respectively.

UR - http://www.scopus.com/inward/record.url?scp=0028132786&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028132786&partnerID=8YFLogxK

U2 - 10.1016/0378-4347(94)00227-4

DO - 10.1016/0378-4347(94)00227-4

M3 - Article

VL - 658

SP - 63

EP - 68

JO - Journal of Chromatography B: Biomedical Sciences and Applications

JF - Journal of Chromatography B: Biomedical Sciences and Applications

SN - 0378-4347

IS - 1

ER -