High-performance liquid chromatographic separation of iron(III) chlorophyllin

Y. Nonomura, M. Yamaguchi, T. Hara, K. Furuya, Naoki Yoshioka, H. Inoue

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The components of iron(III) chlorophyllin were separated by high-performance liquid chromatography on a reversed-phase Inertsil ODS column. Good retention was achieved with acetonitrile-phosphate buffer (pH 2) (60:40, v/v) containing 0.01 M tetramethylammonium chloride (TMACl) in studies of the chromatographic behaviour with various elution solvents and their mixtures. Three kinds of iron(III) chlorophyll derivatives, i.e., iron(III) pheophorbide a (Fe-Phed a), iron(III) chlorin e6 (Fe-Chl e6) and iron(III) chlorin e4 (Fe-Chl e4), were detected as major components. Standard materials for qualitative and quantitative analysis were derived from chlorophyll a which was extracted from spinach. The required compounds were prepared under strict conditions by the insert reaction of iron(II) chloride with pheophytins to avoid molecular degradation by light or molecular oxygen.

Original languageEnglish
Pages (from-to)350-354
Number of pages5
JournalJournal of Chromatography A
Volume721
Issue number2
DOIs
Publication statusPublished - 1996 Jan

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Iron
Liquids
Pheophytins
Spinacia oleracea
Molecular oxygen
High performance liquid chromatography
Chlorophyll
Chlorides
Buffers
Phosphates
High Pressure Liquid Chromatography
Oxygen
Derivatives
Light
Degradation
chlorophyllin
Chemical analysis
ferric chlorin

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

High-performance liquid chromatographic separation of iron(III) chlorophyllin. / Nonomura, Y.; Yamaguchi, M.; Hara, T.; Furuya, K.; Yoshioka, Naoki; Inoue, H.

In: Journal of Chromatography A, Vol. 721, No. 2, 01.1996, p. 350-354.

Research output: Contribution to journalArticle

Nonomura, Y. ; Yamaguchi, M. ; Hara, T. ; Furuya, K. ; Yoshioka, Naoki ; Inoue, H. / High-performance liquid chromatographic separation of iron(III) chlorophyllin. In: Journal of Chromatography A. 1996 ; Vol. 721, No. 2. pp. 350-354.
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