TY - JOUR
T1 - High-performance liquid chromatographic separation of iron(III) chlorophyllin
AU - Nonomura, Y.
AU - Yamaguchi, M.
AU - Hara, T.
AU - Furuya, K.
AU - Yoshioka, N.
AU - Inoue, H.
N1 - Funding Information:
Mass spectrometricm easurementws ere performed by Nihon Petroleum. This work was partially supported by a Grant-in-Aid for Scientific Research,N o. 06453071f,r om the Ministry of Education, Science and Culture, Japan.
PY - 1996/1
Y1 - 1996/1
N2 - The components of iron(III) chlorophyllin were separated by high-performance liquid chromatography on a reversed-phase Inertsil ODS column. Good retention was achieved with acetonitrile-phosphate buffer (pH 2) (60:40, v/v) containing 0.01 M tetramethylammonium chloride (TMACl) in studies of the chromatographic behaviour with various elution solvents and their mixtures. Three kinds of iron(III) chlorophyll derivatives, i.e., iron(III) pheophorbide a (Fe-Phed a), iron(III) chlorin e6 (Fe-Chl e6) and iron(III) chlorin e4 (Fe-Chl e4), were detected as major components. Standard materials for qualitative and quantitative analysis were derived from chlorophyll a which was extracted from spinach. The required compounds were prepared under strict conditions by the insert reaction of iron(II) chloride with pheophytins to avoid molecular degradation by light or molecular oxygen.
AB - The components of iron(III) chlorophyllin were separated by high-performance liquid chromatography on a reversed-phase Inertsil ODS column. Good retention was achieved with acetonitrile-phosphate buffer (pH 2) (60:40, v/v) containing 0.01 M tetramethylammonium chloride (TMACl) in studies of the chromatographic behaviour with various elution solvents and their mixtures. Three kinds of iron(III) chlorophyll derivatives, i.e., iron(III) pheophorbide a (Fe-Phed a), iron(III) chlorin e6 (Fe-Chl e6) and iron(III) chlorin e4 (Fe-Chl e4), were detected as major components. Standard materials for qualitative and quantitative analysis were derived from chlorophyll a which was extracted from spinach. The required compounds were prepared under strict conditions by the insert reaction of iron(II) chloride with pheophytins to avoid molecular degradation by light or molecular oxygen.
UR - http://www.scopus.com/inward/record.url?scp=0030050030&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030050030&partnerID=8YFLogxK
U2 - 10.1016/0021-9673(95)00775-X
DO - 10.1016/0021-9673(95)00775-X
M3 - Article
AN - SCOPUS:0030050030
SN - 0021-9673
VL - 721
SP - 350
EP - 354
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 2
ER -