Abstract
A total of 268 nonhuman primates (20 species) kept in the Primate Research Institute, Kyoto University, Japan, were surveyed for intestinal amebas. Total positive rates as based on the presence of cysts in the stool following formalin-ether sedimentation were as follows: Entamoeba histolytica/E. dispar, 53%; E. coli, 34%; E. hartmanni, 34%; Iodamoeba buetschlii, 25%; Endolimax nana, 8%; and E. chattoni, 3%. Positive rates were higher in Old World monkeys and lower in New World monkeys. All the 141 E. histolytica/E. dispar-positive animals were Macaca monkeys. The E. histolytica/E, dispar-positive samples were analyzed by polymerase chain reaction (PCR) for identification of E. histolytica and E. dispar. E. dispar DNA was detected in 137 samples, whereas no E. histolytica DNA was seen. Zymodeme analysis and reactivity to monoclonal antibodies of cultured trophozoites also supported the presence of E. dispar and the absence of E. histolytica. When the sera of 93 macaques were examined by an indirect fluorescent antibody test, only 3 animals proved to be positive for E. histolytica, showing the lowest titer. These results demonstrate that infection with E. dispar, but not E. histolytica, is predominant in macaques.
| Original language | English |
|---|---|
| Pages (from-to) | 14-17 |
| Number of pages | 4 |
| Journal | Parasitology Research |
| Volume | 87 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2001 |
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ASJC Scopus subject areas
- Parasitology
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High prevalence of infection with Entamoeba dispar, but not E. histolytica, in captive macaques. / Tachibana, Hiroshi; Cheng, Xun Jia; Kobayashi, Seiki; Matsubayashi, Nobuko; Gotoh, Shunji; Matsubayashi, Kiyoaki.
In: Parasitology Research, Vol. 87, No. 1, 2001, p. 14-17.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - High prevalence of infection with Entamoeba dispar, but not E. histolytica, in captive macaques
AU - Tachibana, Hiroshi
AU - Cheng, Xun Jia
AU - Kobayashi, Seiki
AU - Matsubayashi, Nobuko
AU - Gotoh, Shunji
AU - Matsubayashi, Kiyoaki
PY - 2001
Y1 - 2001
N2 - A total of 268 nonhuman primates (20 species) kept in the Primate Research Institute, Kyoto University, Japan, were surveyed for intestinal amebas. Total positive rates as based on the presence of cysts in the stool following formalin-ether sedimentation were as follows: Entamoeba histolytica/E. dispar, 53%; E. coli, 34%; E. hartmanni, 34%; Iodamoeba buetschlii, 25%; Endolimax nana, 8%; and E. chattoni, 3%. Positive rates were higher in Old World monkeys and lower in New World monkeys. All the 141 E. histolytica/E. dispar-positive animals were Macaca monkeys. The E. histolytica/E, dispar-positive samples were analyzed by polymerase chain reaction (PCR) for identification of E. histolytica and E. dispar. E. dispar DNA was detected in 137 samples, whereas no E. histolytica DNA was seen. Zymodeme analysis and reactivity to monoclonal antibodies of cultured trophozoites also supported the presence of E. dispar and the absence of E. histolytica. When the sera of 93 macaques were examined by an indirect fluorescent antibody test, only 3 animals proved to be positive for E. histolytica, showing the lowest titer. These results demonstrate that infection with E. dispar, but not E. histolytica, is predominant in macaques.
AB - A total of 268 nonhuman primates (20 species) kept in the Primate Research Institute, Kyoto University, Japan, were surveyed for intestinal amebas. Total positive rates as based on the presence of cysts in the stool following formalin-ether sedimentation were as follows: Entamoeba histolytica/E. dispar, 53%; E. coli, 34%; E. hartmanni, 34%; Iodamoeba buetschlii, 25%; Endolimax nana, 8%; and E. chattoni, 3%. Positive rates were higher in Old World monkeys and lower in New World monkeys. All the 141 E. histolytica/E. dispar-positive animals were Macaca monkeys. The E. histolytica/E, dispar-positive samples were analyzed by polymerase chain reaction (PCR) for identification of E. histolytica and E. dispar. E. dispar DNA was detected in 137 samples, whereas no E. histolytica DNA was seen. Zymodeme analysis and reactivity to monoclonal antibodies of cultured trophozoites also supported the presence of E. dispar and the absence of E. histolytica. When the sera of 93 macaques were examined by an indirect fluorescent antibody test, only 3 animals proved to be positive for E. histolytica, showing the lowest titer. These results demonstrate that infection with E. dispar, but not E. histolytica, is predominant in macaques.
UR - http://www.scopus.com/inward/record.url?scp=0034895834&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034895834&partnerID=8YFLogxK
U2 - 10.1007/s004360000289
DO - 10.1007/s004360000289
M3 - Article
C2 - 11199843
AN - SCOPUS:0034895834
VL - 87
SP - 14
EP - 17
JO - Zeitschrift fur Parasitenkunde
JF - Zeitschrift fur Parasitenkunde
SN - 0044-3255
IS - 1
ER -