High prevalence of infection with Entamoeba dispar, but not E. histolytica, in captive macaques

Hiroshi Tachibana, Xun Jia Cheng, Seiki Kobayashi, Nobuko Matsubayashi, Shunji Gotoh, Kiyoaki Matsubayashi

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A total of 268 nonhuman primates (20 species) kept in the Primate Research Institute, Kyoto University, Japan, were surveyed for intestinal amebas. Total positive rates as based on the presence of cysts in the stool following formalin-ether sedimentation were as follows: Entamoeba histolytica/E. dispar, 53%; E. coli, 34%; E. hartmanni, 34%; Iodamoeba buetschlii, 25%; Endolimax nana, 8%; and E. chattoni, 3%. Positive rates were higher in Old World monkeys and lower in New World monkeys. All the 141 E. histolytica/E. dispar-positive animals were Macaca monkeys. The E. histolytica/E, dispar-positive samples were analyzed by polymerase chain reaction (PCR) for identification of E. histolytica and E. dispar. E. dispar DNA was detected in 137 samples, whereas no E. histolytica DNA was seen. Zymodeme analysis and reactivity to monoclonal antibodies of cultured trophozoites also supported the presence of E. dispar and the absence of E. histolytica. When the sera of 93 macaques were examined by an indirect fluorescent antibody test, only 3 animals proved to be positive for E. histolytica, showing the lowest titer. These results demonstrate that infection with E. dispar, but not E. histolytica, is predominant in macaques.

Original languageEnglish
Pages (from-to)14-17
Number of pages4
JournalParasitology Research
Volume87
Issue number1
DOIs
Publication statusPublished - 2001

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Entamoeba
Entamoeba histolytica
Macaca
Infection
infection
Primates
Endolimax
Platyrrhini
Cercopithecidae
Trophozoites
Cebidae
trophozoites
Amoeba
Entamoeba dispar
DNA
formalin
Ether
Formaldehyde
Haplorhini
monkeys

ASJC Scopus subject areas

  • Parasitology

Cite this

Tachibana, H., Cheng, X. J., Kobayashi, S., Matsubayashi, N., Gotoh, S., & Matsubayashi, K. (2001). High prevalence of infection with Entamoeba dispar, but not E. histolytica, in captive macaques. Parasitology Research, 87(1), 14-17. https://doi.org/10.1007/s004360000289

High prevalence of infection with Entamoeba dispar, but not E. histolytica, in captive macaques. / Tachibana, Hiroshi; Cheng, Xun Jia; Kobayashi, Seiki; Matsubayashi, Nobuko; Gotoh, Shunji; Matsubayashi, Kiyoaki.

In: Parasitology Research, Vol. 87, No. 1, 2001, p. 14-17.

Research output: Contribution to journalArticle

Tachibana, H, Cheng, XJ, Kobayashi, S, Matsubayashi, N, Gotoh, S & Matsubayashi, K 2001, 'High prevalence of infection with Entamoeba dispar, but not E. histolytica, in captive macaques', Parasitology Research, vol. 87, no. 1, pp. 14-17. https://doi.org/10.1007/s004360000289
Tachibana, Hiroshi ; Cheng, Xun Jia ; Kobayashi, Seiki ; Matsubayashi, Nobuko ; Gotoh, Shunji ; Matsubayashi, Kiyoaki. / High prevalence of infection with Entamoeba dispar, but not E. histolytica, in captive macaques. In: Parasitology Research. 2001 ; Vol. 87, No. 1. pp. 14-17.
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