TY - JOUR
T1 - High prevalence of infection with Entamoeba dispar, but not E. histolytica, in captive macaques
AU - Tachibana, Hiroshi
AU - Cheng, Xun Jia
AU - Kobayashi, Seiki
AU - Matsubayashi, Nobuko
AU - Gotoh, Shunji
AU - Matsubayashi, Kiyoaki
N1 - Funding Information:
Acknowledgements We thank Mr. N. Miwa for the supply of serum samples. We also thank Drs. Y. Kaneda, T. Takeuchi, and T. Enomoto for their encouragement of this study and Dr. W. Stahl for reviewing the manuscript. This study was performed as a part of The Cooperative Research Program of Primate Research Institute, Kyoto University. This work was also supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan and by Health Sciences Research Grants from the Ministry of Health and Welfare of Japan.
PY - 2001
Y1 - 2001
N2 - A total of 268 nonhuman primates (20 species) kept in the Primate Research Institute, Kyoto University, Japan, were surveyed for intestinal amebas. Total positive rates as based on the presence of cysts in the stool following formalin-ether sedimentation were as follows: Entamoeba histolytica/E. dispar, 53%; E. coli, 34%; E. hartmanni, 34%; Iodamoeba buetschlii, 25%; Endolimax nana, 8%; and E. chattoni, 3%. Positive rates were higher in Old World monkeys and lower in New World monkeys. All the 141 E. histolytica/E. dispar-positive animals were Macaca monkeys. The E. histolytica/E, dispar-positive samples were analyzed by polymerase chain reaction (PCR) for identification of E. histolytica and E. dispar. E. dispar DNA was detected in 137 samples, whereas no E. histolytica DNA was seen. Zymodeme analysis and reactivity to monoclonal antibodies of cultured trophozoites also supported the presence of E. dispar and the absence of E. histolytica. When the sera of 93 macaques were examined by an indirect fluorescent antibody test, only 3 animals proved to be positive for E. histolytica, showing the lowest titer. These results demonstrate that infection with E. dispar, but not E. histolytica, is predominant in macaques.
AB - A total of 268 nonhuman primates (20 species) kept in the Primate Research Institute, Kyoto University, Japan, were surveyed for intestinal amebas. Total positive rates as based on the presence of cysts in the stool following formalin-ether sedimentation were as follows: Entamoeba histolytica/E. dispar, 53%; E. coli, 34%; E. hartmanni, 34%; Iodamoeba buetschlii, 25%; Endolimax nana, 8%; and E. chattoni, 3%. Positive rates were higher in Old World monkeys and lower in New World monkeys. All the 141 E. histolytica/E. dispar-positive animals were Macaca monkeys. The E. histolytica/E, dispar-positive samples were analyzed by polymerase chain reaction (PCR) for identification of E. histolytica and E. dispar. E. dispar DNA was detected in 137 samples, whereas no E. histolytica DNA was seen. Zymodeme analysis and reactivity to monoclonal antibodies of cultured trophozoites also supported the presence of E. dispar and the absence of E. histolytica. When the sera of 93 macaques were examined by an indirect fluorescent antibody test, only 3 animals proved to be positive for E. histolytica, showing the lowest titer. These results demonstrate that infection with E. dispar, but not E. histolytica, is predominant in macaques.
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U2 - 10.1007/s004360000289
DO - 10.1007/s004360000289
M3 - Article
C2 - 11199843
AN - SCOPUS:0034895834
VL - 87
SP - 14
EP - 17
JO - Zeitschrift fur Parasitenkunde
JF - Zeitschrift fur Parasitenkunde
SN - 0044-3255
IS - 1
ER -
