High-resolution fluorescence microscopy based on a cyclic sequential multiphoton process

Keisuke Isobe, Akira Suda, Hiroshi Hashimoto, Fumihiko Kannari, Hiroyuki Kawano, Hideaki Mizuno, Atsushi Miyawaki, Katsumi Midorikawa

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We demonstrate high-resolution fluorescence microscopy based on a cyclic sequential multiphoton (CSM) process, which gives rise to fluorescence emission following a sequence of cyclic transitions between the bright and dark states of a fluorophore induced by pump and reverse light. By temporally modulating the reverse intensity, we can extract the fluorescence signal generated through the CSM process. We show that the demodulated fluorescence signal is nonlinearly proportional to the excitation intensities and it gives a higher spatial resolution than that of a confocal microscope.

Original languageEnglish
Pages (from-to)791-797
Number of pages7
JournalBiomedical Optics Express
Volume1
Issue number3
DOIs
Publication statusPublished - 2010 Oct 1

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Fluorescence Microscopy
Fluorescence
microscopy
fluorescence
high resolution
Light
spatial resolution
microscopes
pumps
excitation

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Biotechnology

Cite this

High-resolution fluorescence microscopy based on a cyclic sequential multiphoton process. / Isobe, Keisuke; Suda, Akira; Hashimoto, Hiroshi; Kannari, Fumihiko; Kawano, Hiroyuki; Mizuno, Hideaki; Miyawaki, Atsushi; Midorikawa, Katsumi.

In: Biomedical Optics Express, Vol. 1, No. 3, 01.10.2010, p. 791-797.

Research output: Contribution to journalArticle

Isobe, K, Suda, A, Hashimoto, H, Kannari, F, Kawano, H, Mizuno, H, Miyawaki, A & Midorikawa, K 2010, 'High-resolution fluorescence microscopy based on a cyclic sequential multiphoton process', Biomedical Optics Express, vol. 1, no. 3, pp. 791-797. https://doi.org/10.1364/BOE.1.000791
Isobe, Keisuke ; Suda, Akira ; Hashimoto, Hiroshi ; Kannari, Fumihiko ; Kawano, Hiroyuki ; Mizuno, Hideaki ; Miyawaki, Atsushi ; Midorikawa, Katsumi. / High-resolution fluorescence microscopy based on a cyclic sequential multiphoton process. In: Biomedical Optics Express. 2010 ; Vol. 1, No. 3. pp. 791-797.
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