High-resolution fluorescence microscopy based on a cyclic sequential multiphoton process

Keisuke Isobe, Akira Suda, Hiroshi Hashimoto, Fumihiko Kannari, Hiroyuki Kawano, Hideaki Mizuno, Atsushi Miyawaki, Katsumi Midorikawa

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    We demonstrate high-resolution fluorescence microscopy based on a cyclic sequential multiphoton (CSM) process, which gives rise to fluorescence emission following a sequence of cyclic transitions between the bright and dark states of a fluorophore induced by pump and reverse light. By temporally modulating the reverse intensity, we can extract the fluorescence signal generated through the CSM process. We show that the demodulated fluorescence signal is nonlinearly proportional to the excitation intensities and it gives a higher spatial resolution than that of a confocal microscope.

    Original languageEnglish
    Pages (from-to)791-797
    Number of pages7
    JournalBiomedical Optics Express
    Volume1
    Issue number3
    DOIs
    Publication statusPublished - 2010 Oct 1

    ASJC Scopus subject areas

    • Biotechnology
    • Atomic and Molecular Physics, and Optics

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  • Cite this

    Isobe, K., Suda, A., Hashimoto, H., Kannari, F., Kawano, H., Mizuno, H., Miyawaki, A., & Midorikawa, K. (2010). High-resolution fluorescence microscopy based on a cyclic sequential multiphoton process. Biomedical Optics Express, 1(3), 791-797. https://doi.org/10.1364/BOE.1.000791