High-resolution fluorescence microscopy based on a cyclic sequential multiphoton process

Keisuke Isobe, Akira Suda, Hiroshi Hashimoto, Fumihiko Kannari, Hiroyuki Kawano, Hideaki Mizuno, Atsushi Miyawaki, Katsumi Midorikawa

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

We demonstrate high-resolution fluorescence microscopy based on a cyclic sequential multiphoton (CSM) process, which gives rise to fluorescence emission following a sequence of cyclic transitions between the bright and dark states of a fluorophore induced by pump and reverse light. By temporally modulating the reverse intensity, we can extract the fluorescence signal generated through the CSM process. We show that the demodulated fluorescence signal is nonlinearly proportional to the excitation intensities and it gives a higher spatial resolution than that of a confocal microscope.

Original languageEnglish
Pages (from-to)791-797
Number of pages7
JournalBiomedical Optics Express
Volume1
Issue number3
DOIs
Publication statusPublished - 2010 Oct 1

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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